In situ Observation of Desorption Reaction of Cytochrome c from Solid/Liquid Interfaces with Slab Optical Waveguide Spectroscopy

To measure the direct electron transfer (DET) reaction of cytochrome c (Cytc) immobilized on a bare ITO electrode after removing the adsorbed molecules, automated solution exchange (ASE) processes were performed to induce their desorption. By fitting the absorbance decay curve observed at the Soret band peak position of Cytc at around 408 nm during the ASE processes with a double exponential equation, the final immobilized fraction was estimated to be 58.6% of the Cytc adsorbed on bare ITO electrodes under the experimental conditions. Cyclic voltammograms (CVs) of Cytc adsorbed on the bare ITO electrodes were measured for 60 min to elucidate the DET activity of immobilized Cytc. After repeated CV measurements, approximately 90% of immobilized Cytc was found to remain from the evaluation based on the coulombic amount of reduction and oxidation peaks. The scan rate dependent peak separation data from the immobilized Cytc between reduction and oxidation peaks in CVs produced 2.7 times larger DET reaction rate constant than that previously reported for the Cytc adsorbed on the bare ITO electrode.

show abstract

<i>In situ</i> Observation of Immobilization of Cytochrome <i>c</i> into Hydrophobic DNA Nano-Film

Matsuda¹,

Okabe²,

Omura³

et al. 2019

IEICE Trans. Electron.

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

In situ Observation of Desorption Reaction of Cytochrome c from Solid/Liquid Interfaces with Slab Optical Waveguide Spectroscopy

Cited by 4 publications

References 12 publications

Analytical chemistry toward on-site diagnostics

Analytical chemistry toward on-site diagnostics

Direct Electron Transfer Reaction of Cytochrome c Immobilized on a Bare ITO Electrode

<i>In situ</i> Observation of Immobilization of Cytochrome <i>c</i> into Hydrophobic DNA Nano-Film

Contact Info

Product

Resources

About