In situ monitoring of chlorophyll fluorescence to assess the synergistic effect of low temperature and high irradiance stresses inSpirulina cultures grown outdoors in photobioreactors

Torzillo, Giuseppe; Accolla, Paola; Pinzani, Edoardo; Masojídek, Jiří

doi:10.1007/bf02178571

Cited by 79 publications

(64 citation statements)

References 28 publications

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“…Low F V /F M values in microalgae mass cultures are generally taken as indicating some degree of photoinhibition. Midday PSII photoinhibition of ≈ 30% has been described in outdoor mass culture with Arthrospira platensis (Vonshak et al, 1994;Vonshak et al, 1996;Torzillo et al, 1996;Torzillo et al, 1998) and Chlorella (Masojídek et al, 2011). Most photoinhibition studies conducted on Scenedesmus obliquus have been performed on cultures grown in the laboratory (Flameling and Kromkamp, 1997;Yang and Gao, 2003).…”

Section: Fluorescence Measurements On Lightadapted Samplesmentioning

confidence: 99%

Photosynthesis of Scenedesmus obliquus in outdoor open thin-layer cascade system in high and low CO2 in Belgium

Marchin

Erpicum

Franck

2015

Journal of Biotechnology

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Two outdoor open thin-layer cascade systems operated as batch cultures with the alga Scenedesmus obliquus were used to compare the productivity and photosynthetic acclimations in control and CO 2 supplemented cultures in relation with the outdoor light irradiance. We found that the culture productivity was limited by CO 2 availability. In the CO 2 supplemented culture, we obtained a productivity of up to 24 g dw.m −2 .day −1 and found a photosynthetic efficiency (value based on the PAR solar radiation energy) of up to 5%. In the CO 2 limited culture, we obtained a productivity of up to 10 g dw.m −2 .day −1 while the photosynthetic efficiency was up to 3.3% and decreased to 2.1% when the integrated daily PAR increased. Fluorescence and oxygen evolution measurements showed that ETR and oxygen evolution light saturation curves, as well as lightdependent O 2 uptake were similar in algal samples from both cultures when the CO 2 limitation was removed. In contrast, we found that CO 2 limitation conducted to a decreased PSII photochemical efficiency and an increased light-induced heat-dissipation in the control culture compared to the CO 2 supplemented culture. These features are in line with a lower light use efficiency and may therefore contribute to the lower productivity observed in absence of CO 2 supplementation in outdoor mass cultures of Scenedesmus obliquus.

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Section: Fluorescence Measurements On Lightadapted Samplesmentioning

confidence: 99%

Photosynthesis of Scenedesmus obliquus in outdoor open thin-layer cascade system in high and low CO2 in Belgium

Marchin

Erpicum

Franck

2015

Journal of Biotechnology

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“…d Before calculating PE and Y dw; E , irradiance data were corrected for transmittivity tubes [43]. e Calculated by Janssen [38].…”

Section: Flat Panel Reactorsmentioning

confidence: 99%

Photobiological hydrogen production: photochemical efficiency and bioreactor design

Akkerman¹,

Janssen

Rocha

et al. 2002

International Journal of Hydrogen Energy

453

199

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Biological production of hydrogen can be carried out by photoautotrophic or photoheterotrophic organisms. Here, the photosystems of both processes are described.The main drawback of the photoautotrophic hydrogen production process is oxygen inhibition. The few e ciencies reported on the conversion of light energy into hydrogen energy are low, less than 1.5% on a solar spectrum basis. However, these can be increased to 3-10%, by the immediate removal of produced oxygen.The photochemical e ciency of hydrogen production can be calculated theoretically, and is estimated to be 10% (on solar spectrum basis) for the photoheterotrophic process. With use of the theoretical photochemical e ciency, and the climatic data on sunlight irradiance at a certain location at a certain moment of the year, the theoretical maximum hydrogen production can be estimated.Data on H2 yields and photochemical e ciency from experiments reported in the literature are summarized. Photochemical e ciencies, essentially based on artiÿcial light, can reach 10% or even more, but only at low light intensities, with associated low-H2 production rates.Some re ections on possible photobioreactors lead to two types of (modiÿed) photobioreactors that might be successful for a large-scale biological hydrogen production. ?

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“…These devices present different characteristics according to the quality of actinic lamps (halogen, red or blue light emitted diodes), sensitivity and measuring units (cuvettes versus fiber). On the other hand, measurements of in vivo chlorophyll fluorescence in algal mass cultures in open or closed photobioreactors are still scarce (Vonshack et al, 1994;Torzillo et al, 1996;Masojídek et al, 1999Masojídek et al, , 2011Kromkamp et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Use of in vivo chlorophyll fluorescence to estimate photosynthetic activity and biomass productivity in microalgae grown in different culture systems

Figueroa

Jerez

Korbee

2013

lajar

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ABSTRACT. In vivo chlorophyll fluorescence associated to Photosystem II is being used to evaluate photosynthetic activity of microalgae grown in different types of photobioreactors; however, controversy on methodology is usual. Several recommendations on the use of chlorophyll fluorescence to estimate electron transport rate and productivity of microalgae grown in thin-layer cascade cultivators and methacrylate cylindrical vessels are included. Different methodologies related to the measure of photosynthetic activity in microalgae are discussed: (1) measurement of light absorption, (2) determination of electron transport rates versus irradiance and (3) use of simplified devices based on pulse amplitude modulated (PAM) fluorescence as Junior PAM or Pocket PAM with optical fiber and optical head as measuring units, respectively. Data comparisons of in vivo chlorophyll fluorescence by using these devices and other PAM fluorometers as Water-PAM in the microalga Chlorella sp. (Chlorophyta) are presented. Estimations of carbon production and productivity by transforming electron transport rate to gross photosynthetic rate (as oxygen evolution) using reported oxygen produced per photons absorbed values and carbon photosynthetic yield based on reported oxygen/carbon ratio are also shown. The limitation of ETR as estimator of photosynthetic and biomass productivity is discussed. Low cost:quality PAMs can promote monitoring of chlorophyll fluorescence in algal biotechnology to estimate the photosynthetic activity and biomass productivity. Keywords: absorptance, biomass productivity, electron transport rate, PAM fluorescence, photosynthetic yield.Uso de la fluorescencia de la clorofila in vivo para estimar la actividad fotosintética y productividad de la biomasa en microalgas crecidas en diferentes sistemas de cultivo RESUMEN. La fluorescencia de la clorofila in vivo asociada al Fotosistema II se emplea para la determinación de la actividad fotosintética en microalgas crecidas en distintos tipos de fotobiorreactores pero existe controversia sobre la metodología más apropiada. En este trabajo se hacen varias recomendaciones sobre su uso para estimar la tasa de transporte electrónico (ETR) y la productividad de la biomasa en algas crecidas en cultivadores de cascada de capa fina y en recipientes cilíndricos de metacrilato. Se discuten diferentes medidas asociadas a la metodología de la actividad fotosintética: (1) medidas de absorción de luz, (2) determinación de ETR versus irradiancia, y (3) uso de equipos simplificados basados en fluorescencia por pulso de amplitud modulada (PAM) como el Junior PAM o Pocket PAM, con fibra óptica o cabezal óptico como unidades de medida, respectivamente. Se comparan los datos de fluorescencia in vivo en la microalga Chlorella sp. (Chlorophyta) con los de otros fluorímetros como el Water PAM. Se estima la producción de carbono y productividad de la biomasa mediante la transformación del ETR en producción bruta de oxígeno usando valores teóricos de oxígeno producido por fotones absorbidos y l...

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In situ monitoring of chlorophyll fluorescence to assess the synergistic effect of low temperature and high irradiance stresses inSpirulina cultures grown outdoors in photobioreactors

Cited by 79 publications

References 28 publications

Photosynthesis of Scenedesmus obliquus in outdoor open thin-layer cascade system in high and low CO2 in Belgium

Photosynthesis of Scenedesmus obliquus in outdoor open thin-layer cascade system in high and low CO2 in Belgium

Photobiological hydrogen production: photochemical efficiency and bioreactor design

Use of in vivo chlorophyll fluorescence to estimate photosynthetic activity and biomass productivity in microalgae grown in different culture systems

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