In Situ Long-Term Reductive Bioimmobilization of Cr(VI) in Groundwater Using Hydrogen Release Compound

Faybishenko, Boris; Hazen, Terry C.; Long, Philip E.; Brodie, Eoin L.; Conrad, Mark E.; Hubbard, Susan S.; Christensen, John N.; Joyner, Dominique C.; Borglin, Sharon; Chakraborty, Romy; Williams, Kenneth H.; Peterson, John; Chen, Jinsong; Brown, S. T.; Tokunaga, Tetsu K.; Wan, Jiamin; Firestone, Mary K.; Newcomer, Darrell R.; Resch, Charles T.; Cantrell, Kirk J.; Willett, A.; Koenigsberg, Stephen S.

doi:10.1021/es801383r

Cited by 90 publications

(99 citation statements)

References 52 publications

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“…The array detected a wide range of taxa not recovered in the clone libraries, although no obvious trends in the relative abundance of these taxa were evident. Collectively, the results agree with those of other recent studies where the 16S microarray was used to detect and monitor the microbial population response to biostimulation (4,15,34). Although it seems unlikely that the array alone can gauge the quantitative response of different taxa, when used in conjunction with general knowledge of the physiological properties of key taxa together with relevant geochemical data, the array can provide accurate assessment of the response of key functional groups (15,34) much more rapidly and with equal or greater robustness than conventional cloning and sequencing.…”

supporting

confidence: 91%

“…We therefore examined average normalized array intensity for phylogenetic groups that include the major taxa that appeared (based on the chemical and clone library data) to be involved in ethanol metabolism. Analogous approaches have been used to assess the response of metalreducing, sulfate-reducing, and methanogenic taxa during biostimulation for U(VI) and Cr(VI) immobilization (15,34). Consistent with expectations, mean array intensities, binned at the family level, for Geobacteraceae (which includes Geobacter and related metal-reducing organisms), Rhodocyclaceae (which includes Dechloromonas and related nitrate-reducers) and Oxalobacteriaceae (which includes Herbaspirillum and related nitrate reducers) increased upon stimulation (Fig.…”

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confidence: 80%

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16S rRNA Gene Microarray Analysis of Microbial Communities in Ethanol-Stimulated Subsurface Sediment

et al. 2011

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A high-density 16S rRNA gene microarray was used to analyze microbial communities in a slurry of ethanolamended, uranium-contaminated subsurface sediment. Of specific interest was the extent to which the microarray could detect temporal patterns in the relative abundance of major metabolic groups (nitrate-reducing, metal-reducing, sulfatereducing, and methanogenic taxa) that were stimulated by ethanol addition. The results show that the microarray, when used in conjunction with geochemical data and knowledge of the physiological properties of relevant taxa, provided accurate assessment of the response of key functional groups to biostimulation.

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confidence: 91%

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confidence: 80%

16S rRNA Gene Microarray Analysis of Microbial Communities in Ethanol-Stimulated Subsurface Sediment

et al. 2011

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“…Neither edible oil nor HRC had been previously used to stimulate uranium reduction, but they have been used to stimulate Cr(VI) reduction (Hazen et al 2005;Faybishenko et al 2008), MTBE degradation (Haas et al 2001), PCE dechlorination and degradation (Borden 2007;Long and Borden 2006), and acid mine drainage remediation (Lindow et al 2005). …”

Section: Introductionmentioning

confidence: 99%

Uranium reduction and microbial community development in response to stimulation with different electron donors

Barlett

Moon

Peacock³

et al. 2012

Biodegradation

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Stimulating microbial reduction of soluble U(VI) to less soluble U(IV) shows promise as an in situ bioremediation strategy for uranium contaminated groundwater, but the optimal electron donors for promoting this process have yet to be identified. The purpose of this study was to better understand how the addition of various electron donors to uraniumcontaminated subsurface sediments affected U(VI) reduction and the composition of the microbial community. The simple electron donors, acetate or lactate, or the more complex donors, hydrogen-release compound (HRC) or vegetable oil, were added to the sediments incubated in flow-through columns. The composition of the microbial communities was evaluated with quantitative PCR probing specific 16S rRNA genes and functional genes, phospholipid fatty acid analysis, and clone libraries. All the electron donors promoted U(VI) removal, even though the composition of the microbial communities was different with each donor. In general, the overall biomass, rather than the specific bacterial species, was the factor most related to U(VI) removal. -Vegetable oil and HRC were more effective in stimulating U(VI) removal than acetate. These results suggest that the addition of more complex organic electron donors could be an excellent option for in situ bioremediation of uranium-contaminated groundwater.

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“…In addition to the chemically mediated methods for contaminant immobilization, bioremediation has demonstrated great promise as an additional strategy to promote in situ sequestration of contaminants [11,[45][46][47][48] by harnessing the metabolisms of plants and microorganisms to detoxify and/or affect the in situ mobility of a given contaminant. The use of prokaryotic and eukaryotic microorganisms has proven effective for metal and radionuclide remediation through the processes of biosorption, bioaccumulation, bioreduction, and biomineralization [49][50][51][52][53][54][55][56][57][58][59][60][61].…”

Section: Introductionmentioning

confidence: 99%

Phosphate-Mediated Remediation of Metals and Radionuclides

Martinez

Beazley

Sobecky

2014

Advances in Ecology

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Worldwide industrialization activities create vast amounts of organic and inorganic waste streams that frequently result in significant soil and groundwater contamination. Metals and radionuclides are of particular concern due to their mobility and longterm persistence in aquatic and terrestrial environments. As the global population increases, the demand for safe, contaminantfree soil and groundwater will increase as will the need for effective and inexpensive remediation strategies. Remediation strategies that include physical and chemical methods (i.e., abiotic) or biological activities have been shown to impede the migration of radionuclide and metal contaminants within soil and groundwater. However, abiotic remediation methods are often too costly owing to the quantities and volumes of soils and/or groundwater requiring treatment. The in situ sequestration of metals and radionuclides mediated by biological activities associated with microbial phosphorus metabolism is a promising and less costly addition to our existing remediation methods. This review highlights the current strategies for abiotic and microbial phosphatemediated techniques for uranium and metal remediation.

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In Situ Long-Term Reductive Bioimmobilization of Cr(VI) in Groundwater Using Hydrogen Release Compound

Abstract: ABSTRACT. The results of a field experiment designed to test the effectiveness of a novel approach for long-term, in situ bioimmobilization of toxic and soluble Cr(VI) in groundwater using

Cited by 90 publications

References 52 publications

16S rRNA Gene Microarray Analysis of Microbial Communities in Ethanol-Stimulated Subsurface Sediment

16S rRNA Gene Microarray Analysis of Microbial Communities in Ethanol-Stimulated Subsurface Sediment

Uranium reduction and microbial community development in response to stimulation with different electron donors

Phosphate-Mediated Remediation of Metals and Radionuclides

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