In situ localization and penetration route of an endophytic bacteria into roots of wheat and the common bean

Velichko, Natalya S.; Bagavova, Arapat R.; Burygin, Gennady L.; Baymiev, Andrei Kh; Pylaev, Timofey; Fedonenko, Yu. P.

doi:10.1016/j.rhisph.2022.100567

Cited by 5 publications

(9 citation statements)

References 34 publications

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“…More lateral and vertical root growth, improvement in water use efficiency and decrease in water use were quantified under ridge sowing compared to bed and flat planting (Hu et al, 2020). Concurrent with 'LASER levelling' instigating regulations in the growth of roots, water use for the metabolic processes of plants under ridge sowing might have been enhanced (Velichko et al, 2022). Optimization of sowing methods and soil manipulation techniques based on carbon emissions are lacking in the aforementioned research work.…”

Section: Discussionmentioning

confidence: 99%

Sustainable production of bread wheat using laser land levelling and sowing methods as potent regulators of carbon sequestration, fuel consumption and water use in the arid environment of Bahawalpur, Pakistan

Shahid

Saleem

et al. 2023

Irrigation and Drainage

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Excessive use of water in wheat is associated with high fuel consumption and the emission of carbon dioxide into the environment. This study was conducted with the objectives to decrease water use and the associated fuel consumption and carbon dioxide emissions and to improve the productivity of wheat on a sustainable basis using LASER levelling and sowing methods as potent modulators of these adversities. The experiment was conducted in the research area of the Agronomic Research Station Bahawalpur over 3 years using a split‐plot experimental design and four replications. Treatments contained LASER levelling in the main plot (L0 = no LASER levelling, L1 = LASER levelling) and sowing methods in split plots (S1 = broadcast, S2 = ridge sowing and S3 = drill sowing). Less water use, fuel consumption and emission of carbon dioxide were quantified under ‘LASER levelling’ and under ‘ridge sowing’ compared to other treatments. Regarding wheat crop productivity, the same treatment combination (LASER levelling and ridge sowing) resulted in more fertile tillers, grains per spike, 1000‐grain weight and grain yield. Therefore, we concluded that ‘LASER levelling’ and ‘ridge sowing’ have the potential to reduce the cost involved and environmental pollution (by reducing fuel consumption) and improve water use efficiency, grain yield and yield components of wheat compared to other treatments. Moreover, strong negative associations of water use, fuel consumption and carbon dioxide emissions with the grain yield of wheat were recorded.

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Section: Discussionmentioning

confidence: 99%

Sustainable production of bread wheat using laser land levelling and sowing methods as potent regulators of carbon sequestration, fuel consumption and water use in the arid environment of Bahawalpur, Pakistan

Shahid

Saleem

et al. 2023

Irrigation and Drainage

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“…In the presence of N, BNF can be reduced, but modifying the microbe to disregard this reduction permits uninterrupted fixation of N, thereby promoting the source of N for sustainable agriculture (Yin et al, 2022). Studies have reported how endophytic symbiotes associate with crop plants intracellularly by living in the roots and/or shoots (Salhi et al, 2022;Velichko et al, 2022).…”

Section: Nitrogen-fixing Bacteriamentioning

confidence: 99%

The potential of biostimulants on soil microbial community: a review

Adedayo,

Babalola

2023

Front. Ind. Microbiol.

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To improve agricultural sustainability, an agriculturally productive system is required to maintain soil fertility and reduce the loss of soil biodiversity. One of the modern technologies employs microbial biostimulants that create abundant agricultural yield with high nutritional values, controlling disadvantages obtained from environmental changes. This review aimed to provide knowledge on the effects of biostimulants on microbial communities’ potential to promote agricultural production. Plant biostimulants are novel materials applied in the farming sector nowadays to improve crop yield and commonly occur in plant seeds and as an alternative to chemical derivative application on crop plants. Microbial biostimulants function as biological inputs to increase crop production and reduce the decomposition of soil. In conclusion, the application of biostimulants necessitates the accomplishment of an absolute choice of beneficial microbes as well as the potential to combat problems that will be encountered later in the agricultural sector.

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“…In order to leverage microbial nitrogen fixation to develop more sustainable agricultural practices, more research is needed to understand the complex interactions between plants and their soil partners 7 , 8 . The study of these interactions has currently relies on molecular and genetic analyses 9 – 12 . Although genomics and proteomics provide an understanding of gene and protein expression in this system, they employ destructive techniques that can not yet provide real-time or holistic spatial information on microbial dynamics in situ.…”

Section: Introductionmentioning

confidence: 99%

Label-free functional analysis of root-associated microbes with dynamic quantitative oblique back-illumination microscopy

Filan,

Green,

Diering

et al. 2024

Sci Rep

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The increasing global demand for food, coupled with concerns about the environmental impact of synthetic fertilizers, underscores the urgency of developing sustainable agricultural practices. Nitrogen-fixing bacteria, known as diazotrophs, offer a potential solution by converting atmospheric nitrogen into bioavailable forms, reducing the reliance on synthetic fertilizers. However, a deeper understanding of their interactions with plants and other microbes is needed. In this study, we introduce a recently developed label-free 3D quantitative phase imaging technology called dynamic quantitative oblique back-illumination microscopy (DqOBM) to assess the functional dynamic activity of diazotrophs in vitro and in situ. Our experiments involved three different diazotrophs (Sinorhizobium meliloti, Azotobacter vinelandii, and Rahnella aquatilis) cultured on media with amendments of carbon and nitrogen sources. Over 5 days, we observed increased dynamics in nutrient-amended media. These results suggest that the observed bacterial dynamics correlate with their metabolic activity. Furthermore, we applied qOBM to visualize microbial dynamics within the root cap and elongation zone of Arabidopsis thaliana primary roots. This allowed us to identify distinct areas of microbial infiltration in plant roots without the need for fluorescent markers. Our findings demonstrate that DqOBM can effectively characterize microbial dynamics and provide insights into plant-microbe interactions in situ, offering a valuable tool for advancing our understanding of sustainable agriculture.

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In situ localization and penetration route of an endophytic bacteria into roots of wheat and the common bean

Cited by 5 publications

References 34 publications

Sustainable production of bread wheat using laser land levelling and sowing methods as potent regulators of carbon sequestration, fuel consumption and water use in the arid environment of Bahawalpur, Pakistan

Sustainable production of bread wheat using laser land levelling and sowing methods as potent regulators of carbon sequestration, fuel consumption and water use in the arid environment of Bahawalpur, Pakistan

The potential of biostimulants on soil microbial community: a review

Label-free functional analysis of root-associated microbes with dynamic quantitative oblique back-illumination microscopy

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