In Situ Labeling of Adherent Cells With Pkh26

The biocompatibility of porous silicon in tissues of the eye

“…Thermal oxidation of the membranes was carried out to stabilise the porous silicon surface and to reduce the degradation rate [5,27]. A colorimetric assay for silicic acid confirmed that thermally-oxidised porous silicon membrane exhibited slower degradation in aqueous solution at physiological pH than a non-treated membrane (Fig.…”

“…Cells attached to porous silicon membranes were stained with PKH26 (Sigma Chemical Co.) according to a modification of the method described by Lee et al [27]. In brief, cells were washed twice with sterile PBS and stained with a 2 Â 10 À6 mM solution of the dye in a volume of 500 ml for 5 min at RT, with gentle agitation.…”