In situ intravenous photodynamic therapy for the systemic eradication of blood stream infections

Raschpichler, Michael; Agel, Michael R.; Pinnapireddy, Shashank Reddy; Duse, Lili; Baghdan, Elias; Schäfer, Jens; Bakowsky, Udo

doi:10.1039/c8pp00267c

Cited by 14 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The good EE results can be explained by the fact that PLGA and curcumin are both soluble in ethyl acetate, which was employed for the nanoparticle preparation process. Moreover, PLGA’s ability to effectively encapsulate curcumin has been demonstrated in previous studies [44,45,46].…”

Section: Resultsmentioning

confidence: 90%

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

et al. 2019

Self Cite

View full text Add to dashboard Cite

Accumulation of photosensitisers in photodynamic therapy in healthy tissues is often the cause of unwanted side effects. Using nanoparticles, improved bioavailability and site-specific drug uptake can be achieved. In this study, curcumin, a natural product with anticancer properties, albeit with poor aqueous solubility, was encapsulated in biodegradable polymeric poly(lactic-co-glycolic acid) (PLGA) nanoparticles (CUR-NP). Dynamic light scattering, laser Doppler anemometry and atomic force microscopy were used to characterise the formulations. Using haemolysis, serum stability and activated partial thromboplastin time tests, the biocompatibility of CUR-NP was assessed. Particle uptake and accumulation were determined by confocal laser scanning microscopy. Therapeutic efficacy of the formulation was tested in SK-OV-3 human ovarian adenocarcinoma cells post low level LED irradiation by determining the generation of reactive oxygen species and cytotoxicity. Pharmacologic inhibitors of cellular uptake pathways were used to identify the particle uptake mechanism. CUR-NP exhibited better physicochemical properties such as stability in the presence of light and improved serum stability compared to free curcumin. In addition, the novel nanoformulation facilitated the use of higher amounts of curcumin and showed strong apoptotic effects on tumour cells.

show abstract

Section: Resultsmentioning

confidence: 90%

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many applications combining natural PSs with nanotechnology have been developed, and these studies are summarized in Table 4 . For instance, curcumin is an improved nanotechnology using many different polymers, such as polylactic acid (PLA) and poly(D,L-lactide-co-glycolide) (PLGA) [ 55 , 167 ], and these polymers improve the hemocompatibility and solubility of curcumin. Liposomes and serum albumin nanocarriers are another example of nanotechnology-based PS applications, and in those studies, hypericin improved the APDT performance and speed [ 168 , 169 ].…”

Section: Emerging Technologies and Solutions To Current Apdt Limitationsmentioning

confidence: 99%

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

2021

View full text Add to dashboard Cite

Health problems and reduced treatment effectiveness due to antimicrobial resistance have become important global problems and are important factors that negatively affect life expectancy. Antimicrobial photodynamic therapy (APDT) is constantly evolving and can minimize this antimicrobial resistance problem. Reactive oxygen species produced when nontoxic photosensitizers are exposed to light are the main functional components of APDT responsible for microbial destruction; therefore, APDT has a broad spectrum of target pathogens, such as bacteria, fungi, and viruses. Various photosensitizers, including natural extracts, compounds, and their synthetic derivatives, are being investigated. The main limitations, such as weak antimicrobial activity against Gram-negative bacteria, solubility, specificity, and cost, encourage the exploration of new photosensitizer candidates. Many additional methods, such as cell surface engineering, cotreatment with membrane-damaging agents, nanotechnology, computational simulation, and sonodynamic therapy, are also being investigated to develop novel APDT methods with improved properties. In this review, we summarize APDT research, focusing on natural photosensitizers used in in vitro and in vivo experimental models. In addition, we describe the limitations observed for natural photosensitizers and the methods developed to counter those limitations with emerging technologies.

show abstract

“…One of these promising mechanisms is the application of light, which itself has a long tradition in the history of medicine, in combination with a photoactive drug collectively termed photodynamic therapy (PDT). The basic principle of PDT is a combination of three otherwise non-toxic components, a photosensitiser, light and oxygen [ 6 , 7 ]. The photosensitiser is a molecule that can be excited by light, mostly due to a conjugated π-electron system.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

et al. 2021

Self Cite

View full text Add to dashboard Cite

Lung cancer is one of the most common causes for a high number of cancer related mortalities worldwide. Therefore, it is important to improve the therapy by finding new targets and developing convenient therapies. One of these novel non-invasive strategies is the combination of pulmonary delivered tetraether liposomes and photodynamic therapy. In this study, liposomal model formulations containing the photosensitiser curcumin were nebulised via two different technologies, vibrating-mesh nebulisation and air-jet nebulisation, and compared with each other. Particle size and ζ-potential of the liposomes were investigated using dynamic light scattering and laser Doppler anemometry, respectively. Furthermore, atomic force microscopy and transmission electron microscopy were used to determine the morphological characteristics. Using a twin glass impinger, suitable aerodynamic properties were observed, with the fine particle fraction of the aerosols being ≤62.7 ± 1.6%. In vitro irradiation experiments on lung carcinoma cells (A549) revealed an excellent cytotoxic response of the nebulised liposomes in which the stabilisation of the lipid bilayer was the determining factor. Internalisation of nebulised curcumin-loaded liposomes was visualised utilising confocal laser scanning microscopy. Based on these results, the pulmonary application of curcumin-loaded tetraether liposomes can be considered as a promising approach for the photodynamic therapy against lung cancer.

show abstract

In situ intravenous photodynamic therapy for the systemic eradication of blood stream infections

Abstract: Scheme representing in situ photodynamic therapy within a blood vessel following intravenous administration of a photosensitiser.

Cited by 14 publications

References 9 publications

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

Contact Info

Product

Resources

About