In situ intercellular mechanics of the bovine outer annulus fibrosus subjected to biaxial strains

Bruehlmann, Sabina B.; Hulme, Paul A.; Duncan, Neil A.

doi:10.1016/s0021-9290(03)00244-6

Cited by 64 publications

(73 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was previously inferred from using cell displacement as a strain marker [14,15], obtaining similar strain magnitudes, and led to the proposal that inter-bundle slipping is the main component of strain within a lamella. Bruehlmann et al, however, concluded that intra-lamella cells are relatively shielded from strains; our results suggest that cells in the inter-bundle space could actually be subjected to high shear.…”

Section: Discussionmentioning

confidence: 69%

“…However, the normal bovine disc is of interest in its own right since it is often used as a model for human discs because of its size and mechanical behaviour [14,26,27]. A second limitation is that the viscoelastic behaviour of the tissue was neglected.…”

Section: Discussionmentioning

confidence: 99%

“…That the microscopic strain is smaller than the macroscopic applied strain is due to the inhomogeneous character of the annulus [14]; the macroscopic strains result from the summation of complex interactions between bundles and lamellae. However, the macroscopic strains in this work were directly measured from the displacement of the micromanipulation stages; the actual strain applied to the free part of the annulus ring was therefore overestimated, because of the compression of the portion of the tissue in contact with the vertical flat hooks (Fig.).…”

Section: Discussionmentioning

confidence: 99%

“…For instance, uni-and bi-axial tests have been used to determine the stressstrain response of the annulus [10,11], down to the level of single lamella [12,13]. Most previous work has, however, inferred the mechanical behaviour of the tissue by tracking cells [14,15] or markers that were glued or photobleached on the tissue [10,[14][15][16], or alternatively relied on relatively low resolution tissue tracking (for instance, 0.2 mm 2 elements [16,17]). …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc

et al. 2016

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc

et al. 2016

View full text Add to dashboard Cite

“…The structural mechanism which facilitates tilting is relative collagen fibre reorientation, or inter-fibre 'sliding' [6]. In circumferential tension, the tilt angle (the angle of the fibres to the transverse plane) decreases as fibres re-orient towards the loading direction; in axial tension the opposite occurs [5,18,31,32]. Tensile deformation of lamellae could therefore be considered a two-stage process: on the initial application of load, the straightening of crimp occurs first, functioning perhaps more as a shock-absorbing mechanism to prevent sudden impact damage to the collagen fibres, similar to in tendons; larger-scale tensile deformation, particularly that which occurs axially in bending, then follows, facilitated by collagen fibre re-orientation.…”

Section: Functionmentioning

confidence: 99%

The elastic fibre network of the human lumbar anulus fibrosus: architecture, mechanical function and potential role in the progression of intervertebral disc degeneration

Smith

Fazzalari

2009

Eur Spine J

View full text Add to dashboard Cite

Elastic fibres are critical constituents of dynamic biological structures that functionally require elasticity and resilience. The network of elastic fibres in the anulus fibrosus of the intervertebral disc is extensive, however until recently, the majority of histological, biochemical and biomechanical studies have focussed on the roles of other extracellular matrix constituents such as collagens and proteoglycans. The resulting lack of detailed descriptions of elastic fibre network architecture and mechanical function has limited understanding of the potentially important contribution made by elastic fibres to healthy disc function and their possible roles in the progression of disc degeneration. In addition, it has made it difficult to postulate what the consequences of elastic fibre related disorders would be for intervertebral disc behaviour, and to develop treatments accordingly. In this paper, we review recent and historical studies which have examined both the structure and the function of the human lumbar anulus fibrosus elastic fibre network, provide a synergistic discussion in an attempt to clarify its potentially critical contribution both to normal intervertebral disc behaviour and the processes relating to its degeneration, and recommend critical areas for future research.

show abstract

Mechanics of oriented electrospun nanofibrous scaffolds for annulus fibrosus tissue engineering

Nerurkar

Elliott

Mauck

2007

Journal Orthopaedic Research

221

210

View full text Add to dashboard Cite

Engineering a functional replacement for the annulus fibrosus (AF) of the intervertebral disc is contingent upon recapitulation of AF structure, composition, and mechanical properties. In this study, we propose a new paradigm for AF tissue engineering that focuses on the reconstitution of anatomic fiber architecture and uses constitutive modeling to evaluate construct function. A modified electrospinning technique was utilized to generate aligned nanofibrous polymer scaffolds for engineering the basic functional unit of the AF, a single lamella. Scaffolds were tested in uniaxial tension at multiple fiber orientations, demonstrating a nonlinear dependence of modulus on fiber angle that mimicked the nonlinearity and anisotropy of native AF. A homogenization model previously applied to native AF successfully described scaffold mechanical response, and parametric studies demonstrated that nonfibrillar matrix, along with fiber connectivity, are key contributors to tensile mechanics for engineered AF. We demonstrated that AF cells orient themselves along the aligned scaffolds and deposit matrix that contributes to construct mechanics under loading conditions relevant to the in vivo environment. The homogenization model was applied to cell-seeded constructs and provided quantitative measures for the evolution of matrix and interfibrillar interactions. Finally, the model demonstrated that at fiber angles of the AF (288-448), engineered material behaved much like native tissue, suggesting that engineered constructs replicate the physiologic behavior of the single AF lamella. Constitutive modeling provides a powerful tool for analysis of engineered AF neo-tissue and native AF tissue alike, highlighting key mechanical design criteria for functional AF tissue engineering. ß

show abstract

In situ intercellular mechanics of the bovine outer annulus fibrosus subjected to biaxial strains

Cited by 64 publications

References 32 publications

Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc

Lamellar and fibre bundle mechanics of the annulus fibrosus in bovine intervertebral disc

The elastic fibre network of the human lumbar anulus fibrosus: architecture, mechanical function and potential role in the progression of intervertebral disc degeneration

Mechanics of oriented electrospun nanofibrous scaffolds for annulus fibrosus tissue engineering

Contact Info

Product

Resources

About