In situ induction of dendritic cell–based T cell tolerance in humanized mice and nonhuman primates

Abstract

Administration of an ICAM-1–specific antibody arrests dendritic cells in a semi-immature state and facilitates antigen-specific T cell tolerance to islet allografts in humanized mice and Rhesus monkeys.

Long-Term Control of Diabetes in Immunosuppressed Nonhuman Primates (NHP) by the Transplantation of Adult Porcine Islets

“…ELISPOT analysis was performed by the method previously described (15). Briefly, the frequencies of IFN-g or IL-17A-secreting antigen-specific T cells in the peripheral blood of nonhuman primates were measured using an ELISPOT kit (Mabtech, Nacka Strand, Sweden).…”

In situ application of hydrogel-type fibrin–islet composite optimized for rapid glycemic control by subcutaneous xenogeneic porcine islet transplantation

“…Simple modification of fibrin carrier system would be greatly beneficial in the clinical success of subcutaneous islet transplantation without needing additional complex manipulations. In addition, combining newly developing immune-modulatory regimens might give a great opportunity for the success of clinical xenogeneic islet transplantation [42]. Additional preclinical investigations are currently underway.…”

The Introduction of Human Heme Oxygenase-1 and Soluble Tumor Necrosis Factor-α Receptor Type I With Human IgG1 Fc in Porcine Islets Prolongs Islet Xenograft Survival in Humanized Mice

“…Humanized mice are considered a powerful tool for the investigation of the human immune response to islet xenografts (17)(18)(19)(20). In this study, we investigated whether overexpression of human HO-1, sTNF-aR-Fc or sTNF-aR-Fc/ HO-1 in porcine islets, using adenoviral vectors, could suppress islet cell death and the inflammatory response under hypoxia or proinflammatory stimuli and whether it could improve the outcome of islet xenografts in humanized mice reconstituted with human CD34…”