In situ increased chemokine expression in human cervical intraepithelial neoplasia

Carrero, Yenddy; Mosquera, Jesús; Callejas, Diana; Álvarez‐Mon, Melchor

doi:10.1016/j.prp.2015.01.002

Cited by 17 publications

(13 citation statements)

References 27 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We further detected the concentration of MCP-1 (the cytokine of the greatest change) in supernatants and observed that MCP-1 levels were lower compared with the direct co-cultured group (p < 0.05, Figure 1D). MCP-1 was representative of M1 macrophage activation [21], but previous studies have demonstrated that MCP-1 is closely related to higher tumour malignancy and poorer prognosis, and serves as an important mediator of tumour immune regulation and growth [22,23]. These results implied that metformin inhibited MCP-1 secretion from RAW264.7 cells, in addition to regulating macrophage polarization and improving the inflammatory state of the tumour microenvironment.…”

Section: Metformin Modulation Of Macrophage Polarization and Monocytementioning

confidence: 97%

Metformin affects the features of a human hepatocellular cell line (HepG2) by regulating macrophage polarization in a co‐culture microenviroment

Chen

Zhang

et al. 2015

Diabetes Metabolism Res

View full text Add to dashboard Cite

Metformin can skew RAW264.7 macrophages toward different phenotypes according to changes in the microenvironment, which may affect the inflammatory conditions mediated by macrophages, induce apoptosis and inhibit the proliferation and migration of HepG2 cells. Notch signalling pathway is a potentially important mechanism in the regulation of metformin on macrophage polarization and the subsequent change of hepatoma cells. Copyright © 2015 John Wiley & Sons, Ltd.

show abstract

Section: Metformin Modulation Of Macrophage Polarization and Monocytementioning

confidence: 97%

Metformin affects the features of a human hepatocellular cell line (HepG2) by regulating macrophage polarization in a co‐culture microenviroment

Chen

Zhang

et al. 2015

Diabetes Metabolism Res

View full text Add to dashboard Cite

show abstract

“…Accumulation of a T cell-enriched inflammatory infiltrate in E7-expressing skin is characteristic of pre-malignant lesions associated with the HPVE7 transgene (9). Hyperplasia associated with E7 expression is likely partly responsible for the accumulation of T cells in human HPV-associated cancers, as the hyperplastic malignant epithelium expresses increased levels of chemokines (22) as is also seen in the mouse (16). Further support for this hypothesis is provided by the observation that non-hyperplastic E7 transgenic skin of the K14E7.Rb mut mouse line does not attract a significant inflammatory infiltrate or express elevated chemokine mRNAs (23).…”

Section: Discussionmentioning

confidence: 99%

HPV16-E7-Specific Activated CD8 T Cells in E7 Transgenic Skin and Skin Grafts

et al. 2017

View full text Add to dashboard Cite

Human papillomavirus (HPV) 16 E7 (E7) protein expression in skin promotes epithelial hyperproliferation and transformation to malignancy. Grafts of murine skin expressing E7 protein as a transgene in keratinocytes are not rejected from immunocompetent recipients, whereas grafts expressing ovalbumin (OVA), with or without coexpression of E7 protein, are promptly rejected, demonstrating that E7-associated non-antigen-specific local immunosuppression is not a major determinant of lack of rejection of E7 transgenic skin. To determine whether failure of rejection of E7 skin grafts is due to failure to attract E7-specific effector T cells, E7- and OVA-specific effector CD8+ T cells, activated in vitro, were transferred to animals bearing E7 transgenic skin grafts. Three days after T cell transfer, E7-specific T cells were present in significantly greater numbers than OVA-specific T cells in the grafted skin on animals bearing recently placed or healed E7 grafts, without graft rejection, and also in the ear skin of E7 transgenic animals, without obvious pathology. E7 and OVA-specific T cells were present in lesser numbers in healed E7 grafts than in recently placed grafts and in lesser numbers in recently placed E7 transgenic epidermal grafts without E7-associated hyperproliferation, derived from E7 transgenic mice with a mutated retinoblastoma gene. These data demonstrate that effector T cells are to some extent attracted to E7 transgenic skin specifically by E7 expression, but in large measure non-specifically by the epithelial proliferation associated with E7 expression, and by the local inflammation produced by grafting. Failure of E7 graft rejection was observed despite trafficking of E7-specific effector T cells to E7-expressing epithelium, a finding of consequence for immunotherapy of HPV 16 E7-associated human cancers.

show abstract

“…Interestingly, within normal tissue IL-8 is more commonly found in macrophages, but in CIN it is more prominent in lymphocytes (11). Evidence is inconclusive whether the highest expression of IL-8 is found in CIN II-CIN III (7,30) or in CIN I (11). It must be noted that the expression of IL-8 is higher in CIN when compared to normal tissues (7,11,24,30,42).…”

Section: Introductionmentioning

confidence: 98%

“…However, reduced IL-1α expression in infected cell lines compared to noninfected cell lines may also be observed (29,50). There is an increase in IL-6 secretion in carcinoma cells (11), as well the enhanced release of IL-8 in immortal cells due to the presence of IL-1α (4,50). IL-6 is a pro-inflammatory immune response mediator, which induces and stimulates humoral immunity (15).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interleukin IL-1α, IL-6, IL-8, IL-10 Expression in Different Staging of Cervical Intraepithelial Neoplasia

Balode

Pilmane

Rezeberga

et al. 2017

Acta Chirurgica Latviensis

View full text Add to dashboard Cite

Summary Introduction. Cervical cancer is the fourth most common form of cancer in women [19]. The precancerous stages are divided into three distinctive stages, labelled cervical intraepithelial neoplasia (CIN) I, II and III. One of the aetiological factors is chronic inflammation in cervical tissue, most often induced by Human papilloma virus (HPV). 88,5% of the patients regress from low grade intraepithelial changes to unchanged epithelium [14]. It has been proposed that cytokine balance plays a key role in the development of high grade epithelial changes (CIN I – CIN III) in the remaining 11.5% of patients, however, the exact trigger of this event remains to be found. Aim of the Study. The aim of the study was to determine three pro-inflammatory (IL-1α, IL-6, IL-8) and one anti-inflammatory (IL-10) interleukin expression in different CIN cervix uteri biopsies. Material and methods. 16 biopsies were obtained with different CIN staging: one with CIN I stage, five with CIN II stage and 10 with CIN III stage. The samples were stained with haematoxylin and eosin and immunohistochemistry for IL-1α, IL-6, IL-8 and IL-10. Slides were evaluated semi-quantitatively grading the intensity of positively stained structures in the visual field. Results. Examination of the samples yielded the following: IL-1α expression increased from CIN II to CIN III in squamous epithelium, while IL-8 expression decreased. A few IL-1α containing inflammatory cells were found in all CIN stages. IL-8 expression in subepithelium and the number of inflammatory cells decreased from CIN II stage to CIN III, although, it increased in the blood vessel endothelium. Conclusions. There was constant moderate expression of both IL-6 and IL-10 during all CIN stages, except for inflammatory cells, where IL-6 expression was high during all stages, yet there were few IL-10 containing cells during CIN. The balanced expression of both cytokines suggests that pro- and anti-inflammatory cytokine balance has an important role in CIN morfopathogenesis. The high expression of IL-6 in inflammatory cells and constant expression trough CIN staging indicates sustentation of chronic inflammation and production of other cytokines, such as IL-8, IL-1α. The variable IL-8 expression and its decrease in CIN III stage suggests the depletion of IL-8 production. The high expression of cytokines in blood vessel endothelium indicates their important role in CIN morfopathogenesis.

show abstract

In situ increased chemokine expression in human cervical intraepithelial neoplasia

Cited by 17 publications

References 27 publications

Metformin affects the features of a human hepatocellular cell line (HepG2) by regulating macrophage polarization in a co‐culture microenviroment

Metformin affects the features of a human hepatocellular cell line (HepG2) by regulating macrophage polarization in a co‐culture microenviroment

HPV16-E7-Specific Activated CD8 T Cells in E7 Transgenic Skin and Skin Grafts

Interleukin IL-1α, IL-6, IL-8, IL-10 Expression in Different Staging of Cervical Intraepithelial Neoplasia

Contact Info

Product

Resources

About