In situ imaging of Sulfobacillus thermosulfidooxidans on pyrite under conditions of variable pH using tapping mode atomic force microscopy

Becker, Thomas; Gorham, Nicole T.; Shiers, D.W.; Watling, H.R.

doi:10.1016/j.procbio.2011.01.014

Cited by 24 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extensive oxidization of metal sulfides by biofilms of Sb. thermosulfidooxidans was indicated by the etch pits appearing within 94 h on pyrite surfaces [13]. All of these studies indicate that the biofilm formation by Sulfobacillus probably leads to a high mineral dissolution rate.…”

Section: Introductionmentioning

confidence: 78%

Enhancement of Biofilm Formation on Pyrite by Sulfobacillus thermosulfidooxidans

Sand

Zhang

2016

Minerals

View full text Add to dashboard Cite

Abstract:Bioleaching is the mobilization of metal cations from insoluble ores by microorganisms. Biofilms can enhance this process. Since Sulfobacillus often appears in leaching heaps or reactors, this genus has aroused attention. In this study, biofilm formation and subsequent pyrite dissolution by the Gram-positive, moderately thermophilic acidophile Sulfobacillus thermosulfidooxidans were investigated. Five strategies, including adjusting initial pH, supplementing an extra energy source or ferric ions, as well as exchanging exhausted medium with fresh medium, were tested for enhancement of its biofilm formation. The results show that regularly exchanging exhausted medium leads to a continuous biofilm development on pyrite. By this way, multiply layered biofilms were observed on pyrite slices, while only monolayer biofilms were visible on pyrite grains. In addition, biofilms were proven to be responsible for pyrite leaching in the early stages.

show abstract

Section: Introductionmentioning

confidence: 78%

Enhancement of Biofilm Formation on Pyrite by Sulfobacillus thermosulfidooxidans

Sand

Zhang

2016

Minerals

View full text Add to dashboard Cite

show abstract

“…Atmospheric CO 2 can be fixed in plants via photosynthesis and assimilated into soils via decomposition and microbial activity (Deng et al, 2016;Antonelli et al, 2018), and autotrophic bacteria play a significant role in carbon sequestration in soil ecosystems (Berg, 2011;Alfreider et al, 2017). Six autotrophic carbon sequestration mechanisms are widespread, including the Calvin-Benson-Bassham (CBB) cycle, the reductive tricarboxylic acid (rTCA) cycle, the reductive acetyl-CoA pathway, and the recently discovered 3-hydroxypropionate-4-hydroxybutyrate (HP-HB) cycles (Berg, 2011;Alfreider et al, 2017). Among them, the CBB cycle is the most prevalent means of CO 2 fixation by autotrophs including autotrophic bacteria (Tabita, 1999;Berg, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Six autotrophic carbon sequestration mechanisms are widespread, including the Calvin-Benson-Bassham (CBB) cycle, the reductive tricarboxylic acid (rTCA) cycle, the reductive acetyl-CoA pathway, and the recently discovered 3-hydroxypropionate-4-hydroxybutyrate (HP-HB) cycles (Berg, 2011;Alfreider et al, 2017). Among them, the CBB cycle is the most prevalent means of CO 2 fixation by autotrophs including autotrophic bacteria (Tabita, 1999;Berg, 2011). The enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is important in the CBB cycle and is in fact the most prominent enzyme on Earth (Raven, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is important in the CBB cycle and is in fact the most prominent enzyme on Earth (Raven, 2013). The cbbL and cbbM genes encoding the large subunit of RuBisCO, with 25 % to 30 % amino acid sequence identity (Tabita et al, 2008), serve as autotroph markers (Berg, 2011;Alfreider et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pyrite oxidization accelerates bacterial carbon sequestration in copper mine tailings

Dong

et al. 2019

Biogeosciences

View full text Add to dashboard Cite

Abstract. Polymetallic mine tailings have great potential as carbon sequestration tools to stabilize atmospheric CO2 concentrations. However, previous studies focused on carbonate mineral precipitation, whereas the role of autotrophic bacteria in mine tailing carbon sequestration has been neglected. In this study, carbon sequestration in two samples of mine tailings treated with FeS2 was evaluated using 13C isotope, pyrosequencing and DNA-based stable isotope probing (SIP) analyses to identify carbon fixers. Mine tailings treated with FeS2 exhibited a higher percentage of 13C atoms (1.76±0.06 % for Yangshanchong and 1.36±0.01 % for Shuimuchong) than did controls over a 14-day incubation, which emphasized the role of autotrophs in carbon sequestration with pyrite addition. Pyrite treatment also led to changes in the composition of bacterial communities, and several autotrophic bacteria increased, including Acidithiobacillus and Sulfobacillus. Furthermore, pyrite addition increased the relative abundance of the dominant genus Sulfobacillus by 8.86 % and 5.99 % in Yangshanchong and Shuimuchong samples, respectively. Furthermore, DNA SIP results indicated a 8.20–16.50 times greater gene copy number for cbbL than cbbM in 13C-labeled heavy fractions, and a Sulfobacillus-like cbbL gene sequence (cbbL-OTU1) accounted for 30.11 %–34.74 % of all cbbL gene sequences in 13C-labeled heavy fractions of mine tailings treated with FeS2. These findings highlight the importance of the cbbL gene in bacterial carbon sequestration and demonstrate the ability of chemoautotrophs to sequester carbon during sulfide mineral oxidation in mine tailings. This study is the first to investigate carbon sequestration by autotrophic bacteria in mine tailings through the use of isotope tracers and DNA SIP.

show abstract

“…Generally, it is well understood that T. ferriooxidans-type microbes will dissolve pyrite and can thereby form jarosite solids [12,13]. For the most part, literature is also agreed that below pH 3 jarosite will form [9,14] while above pH 3 and below pH 5 schwertmannite is the product formed.…”

Section: Introductionmentioning

confidence: 99%

Crystallization of jarosite in the presence of amino acids

Crabbe

Fernandez

Jones

2015

Journal of Crystal Growth

View full text Add to dashboard Cite

a b s t r a c tJarosite was formed in the presence of five amino acids at two pHs, namely 1.75 and 2.9, to determine what impact amino acids have on its formation. It was found that at the lower pH glycine was the most potent in terms of morphological and yield impacts. XRD analysis showed that incorporation of the amino acid occurs at this low pH for glycine and proline. Dynamic light scattering studies showed that glycine impacts significantly on the jarosite nucleation rate while proline and alanine do not. At the higher pH all of the amino acids had much less impact on morphology or yield. At pH 3 the solids were found to be a 3-phase system consisting of goethite, schwertmannite and jarosite. In this case, alanine appeared to stabilise the presence of schwertmannite more than the other amino acids.

show abstract

In situ imaging of Sulfobacillus thermosulfidooxidans on pyrite under conditions of variable pH using tapping mode atomic force microscopy

Cited by 24 publications

References 28 publications

Enhancement of Biofilm Formation on Pyrite by Sulfobacillus thermosulfidooxidans

Enhancement of Biofilm Formation on Pyrite by Sulfobacillus thermosulfidooxidans

Pyrite oxidization accelerates bacterial carbon sequestration in copper mine tailings

Crystallization of jarosite in the presence of amino acids

Contact Info

Product

Resources

About