In situ imaging of micropatterned phospholipid membranes by surface plasmon fluorescence microscopy

Tawa, Keiko; Morigaki, Kenichi

doi:10.1016/j.colsurfb.2010.07.038

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…A variety of patterning techniques have been used to produce such systems [41,42]. Here, we consider substrate surfaces that contain two types of surface domains: a substrate surface “matrix” that attracts the membrane relatively strongly and embedded surface domains that attract the membrane only weakly.…”

Section: Membrane Adhesion and Segmentationmentioning

confidence: 99%

Adhesion-Induced Phase Behavior of Two-Component Membranes and Vesicles

Rouhiparkouhi

Weikl

Discher

et al. 2013

IJMS

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The interplay of adhesion and phase separation is studied theoretically for two-component membranes that can phase separate into two fluid phases such as liquid-ordered and liquid-disordered phases. Many adhesion geometries provide two different environments for these membranes and then partition the membranes into two segments that differ in their composition. Examples are provided by adhering vesicles, by hole- or pore-spanning membranes, and by membranes supported by chemically patterned surfaces. Generalizing a lattice model for binary mixtures to these adhesion geometries, we show that the phase behavior of the adhering membranes depends, apart from composition and temperature, on two additional parameters, the area fraction of one membrane segment and the affinity contrast between the two segments. For the generic case of non-vanishing affinity contrast, the adhering membranes undergo two distinct phase transitions and the phase diagrams in the composition/temperature plane have a generic topology that consists of two two-phase coexistence regions separated by an intermediate one-phase region. As a consequence, phase separation and domain formation is predicted to occur separately in each of the two membrane segments but not in both segments simultaneously. Furthermore, adhesion is also predicted to suppress the phase separation process for certain regions of the phase diagrams. These generic features of the adhesion-induced phase behavior are accessible to experiment.

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Section: Membrane Adhesion and Segmentationmentioning

confidence: 99%

Adhesion-Induced Phase Behavior of Two-Component Membranes and Vesicles

Rouhiparkouhi

Weikl

Discher

et al. 2013

IJMS

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“…86 surface plasmon fluorescence microscopy (SPFM) for in situ imaging of micropatterned phospholipid membranes. 87 X-ray and Neutron Techniques. The neutron and X-ray scattering techniques can indirectly probe materials with a spatial resolution situated between crystallography and optical microscopy.…”

Section: ■ Methods Overviewmentioning

confidence: 99%

“…Wang et al reported a combined electrochemistry/SPR study to characterize enzymatic reactions in a supported lipid bilayer . Tawa et al described the use of surface plasmon fluorescence microscopy (SPFM) for in situ imaging of micropatterned phospholipid membranes …”

Section: Methods Overviewmentioning

confidence: 99%

Instrumental Methods to Characterize Molecular Phospholipid Films on Solid Supports

Gözen

Jesorka

2012

Anal. Chem.

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“…Another possible method to construct a porous barrier structure is to use a photomask to control the exposure to UV light and therefore control the crosslinked region. 19,32 However, during photolithography, the smallest feature size is limited by the light diffraction limit; therefore, it is difficult to have a cutoff size smaller than submicron dimensions. The typical membrane-embedded biomolecule sizes are typically much smaller than submicron dimensions, and it would be difficult to use photolithography to construct a barrier with a suitable cutoff size for filtering membrane-embedded biomolecules.…”

Section: Filtering Model Membrane Species In Slbsmentioning

confidence: 99%

Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species

Chen

Chao

2014

Biomicrofluidics

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Supported lipid bilayer (SLB) platforms have been developed to transport and separate membrane-embedded species in the species' native bilayer environment. In this study, we used the phase segregation phenomenon of lipid mixtures containing a polymerizable diacetylene phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC), and a nonpolymerizable phospholipid, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), to create filter barrier structures in SLBs. Upon exposing the phase segregated samples to UV light, the DiynePC-rich domains could become crosslinked and remain fixed on the surface of the support, while the DOPC-rich regions, where no crosslinking could happen, could be removed later by detergent washing, and thus became the void regions in the filter. During the filter fabrication process, we used the laminar flow configuration in a microfluidic channel to control the spatial locations of the feed region and filter region in the SLB. The flow in a microfluidic channel was also used to apply a strong hydrodynamic shear stress to the SLB to transport the membrane-embedded species from the feed region to the filter region. We varied the DiynePC/DOPC molar ratio from 60/40 to 80/20 to adjust the cutoff size of the filter barriers and used two model membrane-embedded species of different sizes to examine the filtering capability. One of the model species, Texas Red 1,2-dihexa-decanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (Texas Red DHPE), had a single-lipid size, and the other species, cholera toxin subunit B-GM1 complex, had a multilipid size. When the DiynePC/DOPC molar ratio was 60/40, both species had high penetration ratios in the filter region. However, when the ratio was increased to 70/30, only the Texas Red DHPE, which was the smaller of the two model species, could penetrate the filter to a considerable extent. When the ratio was increased to 80/20, neither of the model species could penetrate the filter region. The results showed the possibility of using phase segregation of a mixture containing a polymerizable lipid and a nonpolymerizable lipid to fabricate filter barrier structures with tunable cutoff sizes in SLBs.

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In situ imaging of micropatterned phospholipid membranes by surface plasmon fluorescence microscopy

Cited by 7 publications

References 20 publications

Adhesion-Induced Phase Behavior of Two-Component Membranes and Vesicles

Adhesion-Induced Phase Behavior of Two-Component Membranes and Vesicles

Instrumental Methods to Characterize Molecular Phospholipid Films on Solid Supports

Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species

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