In situ identification of mononuclear cells in cutaneous infiltrates in discoid lupus erythematosus, sarcoidosis and secondary syphilis

Abstract

The inflammatory mononuclear cell infiltrates observed in lesional skin from patients with discoid lupus erythematosus (DLE), sarcoidosis, and secondary syphilis have been characterized in situ. Immunological markers (human T lymphocyte antigens and receptors for sheep erythrocytes, C3b, C3d and Fc gamma) were studied by using immunofluorescence tests with IgG F (ab´)2 preparation of anti-T lymphocyte serum, hemadsorption with tissue sections, and tests with soluble immune complexes of peroxidase. In DLE, T lymphocytes were the dominant cell type. In sarcoidosis, the epithelioid cells, including giant cells, had markers similar to macrophages. The lymphohistiocytic cells consisted mainly of macrophages, some T lymphocytes and a few B lymphocytes. In secondary syphilis (condylomata lata), macrophages and T lymphocytes were the dominant cell types, and relatively few B lymphocytes were detected.

Immunophenotyping of the dermal cell infiltrate in lichen planus treated with PUVA

“…Such ANAE-negative T cells have been purified from human adenoids and tonsils in high percentages (18). The percentage of OKT-3 + T cells in the LP lesions was close to 60, which is in agreement with the figures (65 %) reported by Braathen et al ( 2), but somewhat lower than that (75 %) by Bjerke (5). No significant changes were observed in the relative proportions of B and T cells following PUVA therapy (Table I).…”

“…This dermal infiltrate consists mainly of lympho cytes, most of them T celJs, and some macrophages (I, 2, 3). By defining the T cell subpopulations in LP lesions with monoclonal antisera, predominance of helper/inducer subset over the suppressor/cytotoxic one has been reported (4,5,6). Other recent studies, however, suggest that the T suppressor/cytotoxic subset would be responsible for the characteristic degenerative lytic process in the basal keratinocytes of LP lesions (7,8).…”

Subpopulations of mononuclear cells in lesions of psoriasis, lichen planus and discoid lupus erythematosus studied using monoclonal antibodies

“…When compared with the valuc� in periph cral blood f r om normal control:, (approximately 35 o/c suppre�sor/cytotoxic cells) (20), therc i!> a reduced number of suppre�sor/cytotoxic T lympho cyte� in lesions of psoriasi� and DLE. Lo� numbers of supprcssor T lymphocytes in psoriatic lcsions were also indicated by the results obtained using �taining for ANAE activity (3).…”

“…The results accord with our previous data obtained using hem adsorption of sheep erythrocytes to cryostat sec tions (4, 5. 6), an immunofluorescence test with a rabbit antiserum against T lymphocytes (3,6) and staining for a-naphthyl acetate esterase (ANAE) activity (3).…”

“…We have previously shown that the dermal mono nuclear cell infiltrates in lesions of psoriasis (3,5). lichen planus (4) and discoid lupus erythcmatosus (DLE) (6) consist of T lymphocytes and macro phages.…”