In Situ Hybridization of the Rat Calbindin D-28

Séquier, J. M.; Hunziker, W.; Weiser, H.; Richards, J. G.

doi:10.1515/9783110846713.520

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“…In the rat MGB, there is a widespread distribution of CB+ cells and fibers in most divisions, but the strongest CB immunoreactivity is present in nuclei of the dorsal division, and only a few weakly labeled cells are found in the ventral division (Friauf, 1994). Other studies of CB immunoreactivity in the rat MGB (Celio, 1990;Sequier et al, 1990;Puelles et al, 1992) report findings consistent with those of Friauf (1994).…”

Section: Comparative Studies Of Calcium-binding Protein Distribution mentioning

confidence: 74%

Complementary expression of parvalbumin and calbindin D‐28k delineates subdivisions of the rabbit medial geniculate body

Venecia

Smelser

Lossman

et al. 1995

J of Comparative Neurology

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The complementary pattern of immunohistochemical staining for the calcium-binding proteins parvalbumin (PV) and calbindin D-28k (CB) was used to delineate four major subdivisions of the rabbit medial geniculate body (MGB). PV immunoreactivity predominates in the ventral and medial divisions, whereas CB-immunoreactive cells characterize the dorsal and internal divisions. The ventral nucleus is strongly PV+ due to dense neuropil labeling and moderately labeled somata. The medial nucleus contains both medium-sized and large PV+ somata, as well as thick PV+ axons and terminals. The wedge-shaped internal nucleus composed of densely labeled CB+ cells, separates the dorsal and ventral nuclei rostrally, and expands caudally to encapsulate the posterior MGV. Large multipolar CB+ neurons with radiate dendrites characterize the dorsal nucleus. The differential expression of PV and CB also distinguishes the deep dorsal and superficial dorsal subnuclei in the dorsal division and a ventrolateral component in the ventral division. A comparison with studies of MGB connectivity in a variety of species suggests that PV immunoreactivity is highest in subdivisions that receive a substantial input from the central nucleus of the inferior colliculus and that project to primary auditory cortex. In contrast, CB immunoreactivity characterizes nuclei that receive input primarily from other sources, such as the paracentral nuclei of the inferior colliculus, the lateral tegmentum, and the spinal cord, and that project to secondary auditory areas. The ability of calcium-binding protein immunohistochemistry to delineate neuronal compartments across indistinct cytoarchitectonic borders makes it a powerful tool for guiding future connectional and physiological studies of the MGB.

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