In situ hybridization chain reaction mediated ultrasensitive enzyme-free and conjugation-free electrochemcial genosensor for BRCA-1 gene in complex matrices

Yang, Hui; Gao, Yang; Wang, Siqi; Qin, You; Xu, Lu; Jin, Duo; Yang, Fan; Zhang, Guojun

doi:10.1016/j.bios.2016.02.011

Cited by 42 publications

(23 citation statements)

References 31 publications

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“…Yang et al [24] reported another DNA electrochemical sensor for BRCA1 using hybridization chain reaction (HCR) (Figure 3a). HCR is as an isothermal amplification methodology, which does not require complicated variable temperature programs nor the participation of enzymes.…”

Section: Electrochemical Biosensing Of Ctdna and Surrogate Markersmentioning

confidence: 99%

“…The methods discussed above show the involvement of unconventional probes such as THMS [20], DCPs [25], PNA [25,31] and tetrahedral DNA [28], and also the use of a wide variety of nucleic acid amplification strategies, including aMSP [28], TdT, and RNase HII amplification [20], HCR [24], PCR [37], and LAMP [38]. Moreover, to meet the challenging characteristics, required in terms of sensitivity and selectivity, some of the developed methods involve the smart coupling of different amplification strategies within the same bioplatform [20].…”

Section: General Conclusion Insights and Future Perspectivesmentioning

confidence: 99%

“…Electrochemical biosensors have been applied mostly to the determinations of tumor-specific mutations and methylation events, and less to ctDNA quantification and cancer-related viral DNA sequences. As shown in Table 1, electrochemical biosensors exhibit wide ranges of sensitivity (LODs ranging from aM [20,24] to μM [32]) and assay times (from less than 10 min [21] to more than 9 h [32]), selectivity even for discrimination of single point mutation [22,23], and methylation processes at single-base resolution [28]. They show also promising practical applicability for the determination in genomic DNA, that has been extracted from a limited number of clinical samples, mostly saliva and plasma, from cancer patients or even directly in serum without previous DNA extraction [30].…”

Section: General Conclusion Insights and Future Perspectivesmentioning

confidence: 99%

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Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and Its Specific Features

Campuzano

Serafín

Gamella

et al. 2019

Sensors

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Nowadays, analyzing circulating tumor DNA (ctDNA), a very small part of circulating free DNA (cfDNA) carried by blood, is considered to be an interesting alternative to conventional single-site tumor tissue biopsies, both to assess tumor burden and provide a more comprehensive snapshot of the time-related and spatial heterogeneity of cancer genetic/epigenetic scenery. The determination of ctDNA and/or mapping its characteristic features, including tumor-specific mutations, chromosomal aberrations, microsatellite alterations, and epigenetic changes, are minimally invasive, powerful and credible biomarkers for early diagnosis, follow-up, prediction of therapy response/resistance, relapse monitoring, and tracking the rise of new mutant subclones, leading to improved cancer outcomes This review provides an outline of advances published in the last five years in electrochemical biosensing of ctDNA and surrogate markers. It emphasizes those strategies that have been successfully applied to real clinical samples. It highlights the unique opportunities they offer to shift the focus of cancer patient management methods from actual decision making, based on clinic-pathological features, to biomarker-driven treatment strategies, based on genotypes and customized targeted therapies. Also highlighted are the unmet hurdles and future key points to guide these devices in the development of liquid biopsy cornerstone tools in routine clinical practice for the diagnosis, prognosis, and therapy response monitoring in cancer patients.

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Section: Electrochemical Biosensing Of Ctdna and Surrogate Markersmentioning

confidence: 99%

Section: General Conclusion Insights and Future Perspectivesmentioning

confidence: 99%

Section: General Conclusion Insights and Future Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and Its Specific Features

Campuzano

Serafín

Gamella

et al. 2019

Sensors

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“…The majority of reported electrochemical genosensors refer to the determination of DNA point mutations in buffer solutions or real samples (Joda et al, 2015;Sun et al, 2015;Yang et al, 2016;Rasheed et al, 2017).…”

Section: Samplementioning

confidence: 99%

Ultrasensitive electrochemical genosensor for direct detection of specific RNA sequences derived from avian influenza viruses present in biological samples

et al. 2019

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An electrochemical genosensor based on an epoxy-phenanthroline–Fe(III)–NH2-ssDNA layer for the detection of RNA derived from Avian Influenza is presented. The biosensor preparation consists of: (I) modification of gold electrodes with aminoethanethiol, (II) modification of the self-assembled monolayer of aminoethanethiol with 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline using “click” chemistry, (III) a first step of complexation of Fe(III) by 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline, (IV) a second step of complexation of Fe(III) by 5,6-epoxy-5,6-dihydro-[1,10]-phenanthroline, (V) immobilization of the single stranded amino-DNA probe via “click” chemistry between epoxy and amino groups. The interactions between the ssDNA probe and RNA targets were explored with Osteryoung Square Wave Voltammetry. The genosensor showed a remarkable detection limit of 3 copies/µL (5 aM) for RNA extracted from A/swan/Poland/305/06 (H5N1) containing a fully complementary sequence. A linear dynamic range for this sequence was observed from 3.0×103 to 3.0×105 [copies/µl]. RNA extracted from A/mallard/Poland/446/09 (H7N7), containing a non-complementary sequence, generated a much weaker response. Moreover, the developed genosensor allows to distinguish RNA present in biological samples having 2, 3 and 4 mismatches. This biosensing approach can become a potential alternative tool for detecting RNA samples in biomedical research and early clinical diagnosis of avian influenza viruses.

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“…HCR是一种立足点介导的 SDA [108] , HCR可有效地避免假阳性结果和交叉污染, 具有超高灵敏度和结构灵活性, 这些优点使得HCR在电化学生物传感器的分析检测中备受青睐 [105] . 利用HCR 扩增技术, Yang等人 [109] 报道了检测乳腺癌基因BRCA-1 的电化学传感器, 检出限量达到1 amol/L, 并准确地检测分析人血清等复杂基质中的BRCA-1基因.…”

Section: 电化学核酸传感器unclassified

Advances in detection of nucleic acid tumor biomarkers via electrochemical biosensors

Lu¹,

Zhao²,

Li³

2019

Sci. Sin.-Vitae

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In situ hybridization chain reaction mediated ultrasensitive enzyme-free and conjugation-free electrochemcial genosensor for BRCA-1 gene in complex matrices

Cited by 42 publications

References 31 publications

Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and Its Specific Features

Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and Its Specific Features

Ultrasensitive electrochemical genosensor for direct detection of specific RNA sequences derived from avian influenza viruses present in biological samples

Advances in detection of nucleic acid tumor biomarkers via electrochemical biosensors

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