In situ hybridization-based detection of microRNAs in human diseases

Zhang, Xinna; Lu, Xiongbin; López-Berestein, Gabriel; Sood, Anil K.; Calin, George A.

doi:10.2478/micrnat-2013-0002

Cited by 6 publications

(7 citation statements)

References 95 publications

(119 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detection methods that incorporate the context of altered expression, such as multiplex ISH/immunohistochemistry assays, may provide additional validation tools for altered miRNA expression. 13 , 93 …”

Section: Discussionmentioning

confidence: 99%

“…In situ hybridization (ISH) assays are a powerful tool to determine altered miRNA expression at single-cell resolution and to assess the contribution of reactive stroma and immune response. 13 , 93 In breast phyllodes tumors, 94 as well as in colorectal 95 and pancreatic cancer, 96 upregulation of miR-21 expression promotes myofibrogenesis and regulates antimetastatic and proapoptotic target genes, including RECK (reversion-inducing cysteine-rich protein with kazal motifs), SPRY1/2 (Sprouty homolog 1/2 of Drosophila gene), PDCD-4 (programed cell death 4), and PTEN . We have recently shown that high levels of miR-21 expression in the stromal compartment in a cohort of 105 early-stage TNBC cases correlated with shorter recurrence-free and breast cancer–specific survival.…”

Section: Mirna Biomarkers and Therapeutic Opportunities In Tnbc Withomentioning

confidence: 99%

See 1 more Smart Citation

Critical analysis of the potential for microRNA biomarkers in breast cancer management

Graveel

Calderone

Westerhuis

et al. 2015

BCTT

View full text Add to dashboard Cite

Breast cancer is a complex and heterogeneous disease. Signaling by estrogen receptor (ER), progesterone receptor (PR), and/or human EGF-like receptor 2 (HER2) is a main driver in the development and progression of a large majority of breast tumors. Molecular characterization of primary tumors has identified major subtypes that correlate with ER/PR/HER2 status, and also subgroup divisions that indicate other molecular and cellular features of the tumors. While some of these research findings have been incorporated into clinical practice, several challenges remain to improve breast cancer management and patient survival, for which the integration of novel biomarkers into current practice should be beneficial. microRNAs (miRNAs) are a class of short non-coding regulatory RNAs with an etiological contribution to breast carcinogenesis. miRNA-based diagnostic and therapeutic applications are rapidly emerging as novel potential approaches to manage and treat breast cancer. Rapid technological development enables specific and sensitive detection of individual miRNAs or the entire miRNome in tissues, blood, and other biological specimens from breast cancer patients. This review focuses on recent miRNA research and its potential to address unmet clinical needs and challenges. The four sections presented discuss miRNA findings in the context of the following clinical challenges: biomarkers for early detection; prognostic and predictive biomarkers for treatment decisions using targeted therapies against ER and HER2; diagnostic and prognostic biomarkers for subgrouping of triple-negative breast cancer, for which there are currently no targeted therapies; and biomarkers for monitoring and characterization of metastatic breast cancer. The review concludes with a critical analysis of the current state of miRNA breast cancer research and the need for further studies using large patient cohorts under well-controlled conditions before considering the clinical implementation of miRNA biomarkers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Mirna Biomarkers and Therapeutic Opportunities In Tnbc Withomentioning

confidence: 99%

Critical analysis of the potential for microRNA biomarkers in breast cancer management

Graveel

Calderone

Westerhuis

et al. 2015

BCTT

View full text Add to dashboard Cite

show abstract

“…In the present study, we evaluated the expression of miR-361-5p by ISH analysis. Compared with other methods, ISH can visualize microRNA expression at the cellular level, so it is the only method that can directly assess the actual distribution of miRNAs (26,27). ISH analysis can thereby provide more precise information.…”

Section: Discussionmentioning

confidence: 99%

Positive expression of miR-361-5p indicates better prognosis for breast cancer patients

et al. 2016

View full text Add to dashboard Cite

Background: MicroRNA-361-5p (miR-361-5p) has been reported to be tumor suppressor in colorectal, gastric and prostate cancer, but as an oncogene in cervical cancer. No previous research has focused on the expression of miR-361-5p and its exact prognostic role in breast cancer (BC). with negative miR-361-5p expression (P=0.002). Moreover, the prognostic value of miR-361-5p was most significant among patients with triple-negative breast cancer (TNBC) for DFS (P=0.004).Conclusions: These results indicated that miR-361-5p expression is an independent predictive factor for better prognosis in BC.

show abstract

“…Formalin fixed paraffin embedded (FFPE) tissues were sectioned at 4 µm for ISH assay. DIG (digoxigenin) labeled anti-sense miR-21 oligonucleotide probe (5'-TCAACATCAG-TCTGATAAGCTA -3') was purchased from Boster (China) and used as the probe in the ISH assay as previously described [11]. Briefly, the FFPE sections were deparaffinized, rehydrated, and blocked for peroxidase activity, followed by proteinase K digestion and PBS (0.5M, pH 7.4) rinsing.…”

Section: In Situ Hybridization (Ish)mentioning

confidence: 99%

The expression of miR-21 in brain glioma cells and its effect of PI3K/AKT signal pathway Running title: MiR-21 in glioma

Liu¹,

Zhang²,

Luo³

2016

J Psychiatry Brain Sci.

View full text Add to dashboard Cite

Background: Brain glioma is a common primary intracranial tumor with unfavorable prognosis. MicroRNA-21 (miR-21) is a small RNA molecule involved in post-transcriptional modulation and is closely associated with cell growth and differentiation. Recent studies have shown that miR-21 expression affects tumor proliferation and growth via the PI3K/ AKT signaling pathway. This study investigated the expression of miR-21 in glioma and its effects on the PI3K/AKT pathway and cell proliferation. Methods: A total of 35 brain glioma patients were recruited from March 2013 to November 2014 at our hospital. In situ hybridization (ISH) and RT-PCR were used to detect the expression of miR-21 in both tumors and adjacent brain tissues. Human brain U251 glioma cells were transfected with anti-miR-21 to suppress miR-21 expression, followed by immunofluorescent assay of PI3K and AKT expression profiles. Flow cytometry was employed to detect the change between different stages of the cell cycle. Results: ISH results showed significantly elevated miR-21 expression level in glioma tissues compared to normal brain tissues. RT-PCR analysis revealed a 3.5-fold increase in miR-21 in the anti-miR-21 transfected glioma cells compared to untransfected cells. After silencing miR-21 in U251 cells, immunofluorescent assay indicated that the expression of PI3K and AKT significantly decreased. Flow cytometry revealed an increased number of cells at G0/G1 phase in anti-miR-21 transfected U251 cells, suggesting suppressed cell division ability. Conclusion: MiR-21 expression was found to be increased in brain glioma tissues, accompanied with over-activation of PI3K/AKT signaling and proliferation of glioma cells.

show abstract

In situ hybridization-based detection of microRNAs in human diseases

Cited by 6 publications

References 95 publications

Critical analysis of the potential for microRNA biomarkers in breast cancer management

Critical analysis of the potential for microRNA biomarkers in breast cancer management

Positive expression of miR-361-5p indicates better prognosis for breast cancer patients

The expression of miR-21 in brain glioma cells and its effect of PI3K/AKT signal pathway Running title: MiR-21 in glioma

Contact Info

Product

Resources

About