In‐situ grown metal organic framework synergistic system for the enantioseparation of three drugs in open tubular capillary electrochromatography

Abstract

Metal organic frameworks have received great attention as the chiral stationary phase for racemic drug separation because of their fascinating structures and properties. However, the most homochiral metal organic frameworks were constructed by rare and precious chiral organic ligands. In this work, an achiral metal organic framework, together with a natural chiral selector carboxymethyl β‐cyclodextrin built a synergistic separation system in the open tubular capillary electrochromatography. The novel coated columns were developed by inducing metal organic framework nanoparticles to grow on the imidazolyl functional capillary inner wall. The baseline separations of hydroxychloroquine, ofloxacin, and atenolol were achieved in the synergistic separation system. The effects of the concentration of chiral selector, pH, voltage, and the concentration of organic additives were studied. Compared with chiral selector auxiliary bare capillary, the resolutions of three drugs were remarkably improved. The relative standard deviations for the retention time of intraday (n = 6), interday (n = 6), and column‐to‐column were less than 2.1, 2.6, and 5.2%, respectively. These results demonstrate that affordable synergistic separation systems are prospective for racemic drug enantioseparation in capillary electrochromatography.

Recent Advances in Separation and Analysis of Chiral Compounds

“…112 The MOF-like metal organic polymer 4,5-IMD-Zn prepared from imidazole-4,5-dicarboxylic acid and Zn(NO 3 ) 2 was covalently bonded on the capillary for enantio-separation of chiral drugs with CM-β-CD as the chiral selector. 113…”

“…The obtained PGMA NP-based column with azithromycin lactobionate and clindamycin phosphate as the chiral selector gave a high column efficiency of the selected drugs (1.2 × 10 5 plates m –1 ) . The MOF-like metal organic polymer 4,5-IMD-Zn prepared from imidazole-4,5-dicarboxylic acid and Zn­(NO 3 ) 2 was covalently bonded on the capillary for enantio-separation of chiral drugs with CM-β-CD as the chiral selector …”

Recent applications and chiral separation development based on stationary phases in open tubular capillary electrochromatography (2019–2022)

“…CD [ 34 , 35 , 100 ] is the most commonly used polysaccharide material for chiral separations and has appeared frequently. It is either a component of a mixed SP or a chiral selective agent used for synergistic separations.…”

“…Overall, we provide readers interested in OT-CEC with the most up-to-date overview of this field, and the related new SPs from 2019 to 2022 are listed in Table 1 [ 3 , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] ]. PubMed and Web of Science were used to search the following characters (also their acronyms) alone or in combination: “Open tubular”, “Capillary electrochromatography”, “Chiral separation”, “Stationary phase”, and “Coating” for relevant literature to analyze recent OT-CEC developments.…”

Cyclodextrin‐NH‐MIL‐53 open tubular stationary phase for capillary electrochromatography enantioseparation

“…The capillary columns commonly used in CEC can be divided into three categories: packed, monolithic, and open-tubular (OT) columns [8]. CEC using OT capillary columns (OT-CEC) offers simple preparation processes, high separation efficiency, and rapid analysis, all of which are clear advantages of the method [9,10]. However, there are limitations to OT-CEC, including small sample capacity and low phase ratio, which have hindered the development and applications of CEC [11].…”