In Situ Fuel Processing in a Microbial Fuel Cell

Bahartan, Karnit; Amir, Liron; Israel, Álvaro; Lichtenstein, Rachel G.; Alfonta, Lital

doi:10.1002/cssc.201200063

Cited by 36 publications

(44 citation statements)

References 39 publications

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“…Nevertheless, it is complicated to co-immobilize two or three enzymes at the same time, as the spatial orientation of the enzymes cannot be controlled. Alfonta et al displayed GA and GOx on yeast surface, respectively, to obtain GA-yeast and GOx-yeast, and then constructed a two-chamber EFC 496 . However, the P max was only about 3 µW cm -2 , probably due to the low catalytic efficiency arising from the spatial barrier between GA and GOx.…”

Section: Efficient Efcs Based On Microbial Surface Displayed Enzyme Amentioning

confidence: 99%

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

et al. 2019

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The ever-increasing demands for clean and sustainable energy sources combined with rapid advances in bio-integrated portable or implantable electronic devices have stimulated intensive research activities in enzymatic (bio)fuel cells (EFCs). The use of renewable biocatalysts, the utilization of abundant green, safe, and high energy density fuels, together with the capability of working at modest and biocompatible conditions, make EFCs promising as next generation alternative power sources. However, the main challenges (low energy density, relatively low power density, poor operational stability and limited voltage output) hinder future applications of EFCs. This review aims at exploring the underlying mechanism of EFCs and providing possible practical strategies, methodologies and insights to tackle of these issues. Firstly, this review summarizes approaches in achieving high energy densities in EFCs, particularly, employing enzyme cascades for the deep/complete oxidation of fuels. Secondly, strategies for increasing power densities in EFCs, including increasing enzyme activities, facilitating electron transfers, employing nanomaterials, and designing more efficient enzyme-electrode interfaces, are described. The potential of EFCs/(super)capacitor combination is discussed. Thirdly, the review evaluates a range of strategies for improving the stability of EFCs, including the use of different enzyme immobilization approaches, tuning enzyme properties, designing protective matrixes, and using microbial surface displaying enzymes. Fourthly, approaches for the improvement of the cell voltage of EFCs are highlighted. Finally, future developments and a prospective on EFCs are envisioned.

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Section: Efficient Efcs Based On Microbial Surface Displayed Enzyme Amentioning

confidence: 99%

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

et al. 2019

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“…Interestingly, microbial fuel cell (MFCs) offers the possibility of obtaining electrical current from microalgae in a one step‐process . Basically, MFCs are bioelectrochemical transducers that convert chemical or light energy into electrical energy via utilizing various microorganisms (exoelectrogens) which are able to transfer electrons extracellularly to the electrode surface with no need for artificial mediators . The existence of the exoelectrogens is very common in bacteria but rare in microalgae, and the mechanism of how microalgae produce bioelectricity is unclear as yet.…”

Section: Introductionmentioning

confidence: 99%

“…3,4 Basically, MFCs are bioelectrochemical transducers that convert chemical or light energy into electrical energy via utilizing various microorganisms (exoelectrogens) which are able to transfer electrons extracellularly to the electrode surface with no need for artificial mediators. 3,[5][6][7][8][9][10] The existence of the exoelectrogens is very common in bacteria but rare in microalgae, and the mechanism of how microalgae produce bioelectricity is unclear as yet. Utilization of microalgae in MFCs has gained interest because phototrophic microalgae act as biocathodes, as the oxygen they produce serves as final electron acceptor, minimizing the energy needed for aeration at the cathode.…”

Section: Introductionmentioning

confidence: 99%

Biosensing of algal‐photosynthetic productivity using nanostructured bioelectrochemical systems

Mahmoud

Abdo

Samhan

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Microalgae have attracted worldwide interest resulting from their extensive applications in renewable energy and biomass production. However, in algal fuel cells, photosynthetically evolved oxygen hinders the use of algal biofilms formed at the anode surface. Here, nanostructured bio‐electrochemical systems have been designed to explore the algal bio‐electrochemistry at different illumination and growth conditions. RESULTS Three algal strains were screened for their exoelectrogenic activity and the possibility of direct electron transfer to the chemically modified surfaces. After adjusting light and dark conditions and medium compositions, oxygen production within the fuel cells was regulated. At the anode surface, the obtained bioelectrochemical responses and the morphological characterizations suggested that Oscillatoria agardhii has the potential to serve as electron donor and the nanostructured surface is the final electron acceptor. To that end, dual‐chamber algal fuel cells were constructed and glucose (10 g L–1) was used as carbon source. CONCLUSION Power density of 26.8 mW m–2 was produced using the biofilm formed by O. agardhii. Eventually, the consumption of organic waste was monitored, whereas the chemical oxygen demand removal reached 82%. © 2019 Society of Chemical Industry

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“…This system was further improved to assemble an artificial biofilm of bacteria surface-displaying ADH [ 11 ]. In situ processing of complexed polysaccharides such as starch into fuel was demonstrated in a hybrid cell by the coupling of yeast displaying the starch-hydrolyzing enzyme glucoamylase with glucose oxidase-displaying yeast in a mixed culture [ 12 ]. A mixed culture, however, cannot be efficiently controlled over time.…”

Section: Introductionmentioning

confidence: 99%

“…The use of enzymatic cascades in enzymatic fuel cell anodes resulted in very high power outputs, as the electron density achieved was much higher when the fuel was fully oxidized; thus all electrons extracted from a fuel molecule could be transferred to the anode [ 14 , 15 , 16 , 17 ]. The different surface-display systems presented in different microorganisms and employed in hybrid fuel cells use the fusion of the surface-displayed enzyme to a surface-displayed protein [ 5 , 8 , 9 , 12 ]. One of the major drawbacks of the hybrid systems is the limitation of only one copy of an enzyme to be surface-displayed per each surface-displayed protein, significantly limiting the number of redox enzymes per yeast cell.…”

Section: Introductionmentioning

confidence: 99%

The Electrosome: A Surface-Displayed Enzymatic Cascade in a Biofuel Cell’s Anode and a High-Density Surface-Displayed Biocathodic Enzyme

et al. 2017

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The limitation of surface-display systems in biofuel cells to a single redox enzyme is a major drawback of hybrid biofuel cells, resulting in a low copy-number of enzymes per yeast cell and a limitation in displaying enzymatic cascades. Here we present the electrosome, a novel surface-display system based on the specific interaction between the cellulosomal scaffoldin protein and a cascade of redox enzymes that allows multiple electron-release by fuel oxidation. The electrosome is composed of two compartments: (i) a hybrid anode, which consists of dockerin-containing enzymes attached specifically to cohesin sites in the scaffoldin to assemble an ethanol oxidation cascade, and (ii) a hybrid cathode, which consists of a dockerin-containing oxygen-reducing enzyme attached in multiple copies to the cohesin-bearing scaffoldin. Each of the two compartments was designed, displayed, and tested separately. The new hybrid cell compartments displayed enhanced performance over traditional biofuel cells; in the anode, the cascade of ethanol oxidation demonstrated higher performance than a cell with just a single enzyme. In the cathode, a higher copy number per yeast cell of the oxygen-reducing enzyme copper oxidase has reduced the effect of competitive inhibition resulting from yeast oxygen consumption. This work paves the way for the assembly of more complex cascades using different enzymes and larger scaffoldins to further improve the performance of hybrid cells.

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In Situ Fuel Processing in a Microbial Fuel Cell

Cited by 36 publications

References 39 publications

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

Tackling the Challenges of Enzymatic (Bio)Fuel Cells

Biosensing of algal‐photosynthetic productivity using nanostructured bioelectrochemical systems

The Electrosome: A Surface-Displayed Enzymatic Cascade in a Biofuel Cell’s Anode and a High-Density Surface-Displayed Biocathodic Enzyme

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