In situ fluorescence of lac dye stabilized gold nanoparticles; DNA binding assay and toxicity study

Pattanayak, Sutanuka; Chakraborty, Sharmila; Mollick, Md. Masud Rahaman; Roy, Indranil; Basu, Samita; Rana, Dipak; Gauri, Samiran S.; Chattopadhyay, Dipankar; Chakraborty, Mukut

doi:10.1039/c6nj00087h

Cited by 19 publications

(7 citation statements)

References 47 publications

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“…4 a. XRD pattern exhibits the diffraction peaks at 2θ = 36.45°, 38.21°, 44.39°, 64.76° and 77.76° corresponds to the Bragg reflection signals at (101), (111), (200), (220) and (311) indicates the standard metallic gold (Au 0 ) [(Joint Committee on Powder Diffraction Standards, USA-JCPS ID # 00-002-1095] with lattice parameters of a = 4.065 Å and the space group of Fm-3 m. This Bragg reflection signals exhibited the face centered cubic (FCC) crystal structure and the shoulder peak was observed around 30–40° which attributed to the highly crystalline nature of AuNPs (Fig. 4 b) 66 , 67 . The size of the nanoparticles was measured indirectly using a broad bottom width of (111) reflection which indicates the smaller size of the nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

An effective strategy for development of docetaxel encapsulated gold nanoformulations for treatment of prostate cancer

Thambiraj

Vijayalakshmi

Shankaran

2021

Sci Rep

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Nanoformulation based drug delivery is one of the most important research areas in the field of nanomedicine, which provides promising alternatives to the limitations of conventional chemotherapy. Nano drug delivery enables improved pharmacokinetic profile, bioavailability and therapeutic efficiency compared to the regular chemotherapeutic drugs. Herein, we have established a simple method for the synthesis of docetaxel (Dtx) encapsulated poly (ethylene glycol) (PEG) functionalized gold nanoparticles (AuNPs) for targeted drug delivery to prostate cancer. AuNPs were synthesized by the citrate ion reduction method followed by functionalization with thiol-PEG-amine (SH-PEG-NH2). SH-PEG-NH2 functionalized AuNPs were conjugated with the targeting vehicle, folic acid (FA). The anticancer drug, Dtx was encapsulated within AuNPs by the non-covalent linkage method. The physicochemical characteristics of the synthesized nanoformulations were extensively characterized by various spectral and microscopic studies. HR-TEM indicates the average size of the AuNPs is 16 nm and the nanoformulations is 18 nm. The encapsulation efficiency of the Dtx is ~ 96% which is confirmed by the elemental mapping analysis. The in vitro drug release profile of Dtx and AuNPs nanoformulations were studied by the dialysis membrane method. The anticancer activity of docetaxel encapsulated AuNPs were evaluated with prostate cancer cell lines (PC3). The drug encapsulated nanoformulations reduced the cell viability to about 40% (40 µM concentration at 24, 48 and 72 h of treatment). The optical microscopy observation reveals that the damage of prostate cancer cells after exposure to Dtx encapsulated AuNPs. The good cytotoxic activity of the present nanoformulation against prostate cancer cell lines enables its application for targeted drug delivery to prostate cancer.

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Section: Resultsmentioning

confidence: 99%

An effective strategy for development of docetaxel encapsulated gold nanoformulations for treatment of prostate cancer

Thambiraj

Vijayalakshmi

Shankaran

2021

Sci Rep

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“…Well known anticancer drugs are cisplatin and carboplatin offer several side effects . In most of the cases, either silver or gold nanoparticles/clusters show the DNA‐binding activity . However, the reports on DNA‐binding ability of yttrium‐based metals are trivial to date.…”

Section: Introductionmentioning

confidence: 73%

Luminescence, Dye Degradation and DNA Binding Properties of a Dinuclear Nona‐Coordinated Y(III) Complex

2018

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In this study, a dinuclear nona-coordinated acetate bridged [Y 2 (μ 3 -CH 3 COO) 2 (CH 3 COO) 4 (OH) 4 ]. 4H 2 O (1) complex was synthesized and its luminescence, dye degradation and DNA binding properties were evaluated. The complex 1 belonged to triclinic crystal system with P-1 space group and the structure consisted of [Y III 2 (μ 3 -OR) 2 (μ-OR) 4 (OH) 4 ] asymmetric "butterfly" units, where R is the rest of a CH 3 CO 2 group linked together in the form of a loop by alternating anti, anti (μ 3 -O 2 CMe) 2 and (μ-O 2 CMe) 2 units attached with the Y wingtip atoms. Interestingly, the coordination number of each Y atom was nine in crystal 1, which was unprecedented and the oxidation states of both the Y atoms were + 3 and confirmed by BVS calculation. The interand intra-molecular H-bonding in the molecule gave rise to a layer type of structure. The luminescence property of the Complex 1 reveals that the emission band arises at 279 nm which indicates the Complex 1 had efficient luminescence activity. The Complex 1 exhibited an exciting performance towards degradation of a model pollutant methylene blue and also showed good DNA-binding ability.

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“…Furthermore, a structure appears in the excitation spectrum of CD‐1 . It exhibits bathochromic shift compared to lac dye [49] from where it originates as caused by the procedure to make CD‐1 . Formation of additional unsaturated moieties in the solvothermal procedure may be discussed as one reason for this phenomenon.…”

Section: Resultsmentioning

confidence: 99%

Distinct Sustainable Carbon Nanodots Enable Free Radical Photopolymerization, Photo‐ATRP and Photo‐CuAAC Chemistry

Kütahya

Zhai

et al. 2021

Angew Chem Int Ed

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In situ fluorescence of lac dye stabilized gold nanoparticles; DNA binding assay and toxicity study

Abstract: In situ synthesis of natural lac stabilized AuNPs, having DNA interactions and antitoxicity properties: UV-Vis and fluorimetric studies.

Cited by 19 publications

References 47 publications

An effective strategy for development of docetaxel encapsulated gold nanoformulations for treatment of prostate cancer

An effective strategy for development of docetaxel encapsulated gold nanoformulations for treatment of prostate cancer

Luminescence, Dye Degradation and DNA Binding Properties of a Dinuclear Nona‐Coordinated Y(III) Complex

Distinct Sustainable Carbon Nanodots Enable Free Radical Photopolymerization, Photo‐ATRP and Photo‐CuAAC Chemistry

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