In situ experiment to evaluate biochemical responses in the freshwater mussel Diplodon chilensis under anthropogenic eutrophication conditions

Yusseppone, María Soledad; Bianchi, Viginia A.; Castro, Juan Manuel; Abad, Tatiana Noya; Minaberry, Yanina Susana; Sabatini, Sebastián E.; Luquet, Carlos Marcelo; Molina, María C. Ríos de; Rocchetta, Iara

doi:10.1016/j.ecoenv.2020.110341

Cited by 7 publications

(3 citation statements)

References 72 publications

(76 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase in soft tissues was previously reported in mussels and clams exposed to municipal effluent in the laboratory ( Nobles and Zhang, 2015 ). In another study, mussel caging was used to determine the ecotoxicological impacts of leachates from an open dump site ( Yusseppone et al, 2020 ). Mussels were caged for over 30 days at these sites and showed signs of stress in the form of lysosome membrane destabilization in the hemocytes; additionally, certain tissues (gills and adductor muscles) exhibited elevated copper and iron levels.…”

Section: Discussionmentioning

confidence: 99%

Comparative toxicity of urban wastewater and rainfall overflow in caged freshwater mussel Elliptio complanata

et al. 2023

View full text Add to dashboard Cite

Municipal effluents are well-recognized as disrupting sexual differentiation and reproduction in mussels. However, the contribution to this problem made by rainfall combined with sewer overflow (increased by rain due to climate change) is not well understood. The purpose of this study was to compare the neuroendocrine effects of municipal discharge and rainfall overflow on caged endemic mussel Elliptio complanata. To this end, mussels were experimentally caged and placed for 3 months at a municipal effluent dispersion plume site and at overflow sites. Data revealed that downstream surface water contained some pharmaceuticals (caffeine and carbamazepine) and accumulated significant levels of heterotrophic bacteria, but these effects were not observed at the overflow sites. The principal effects observed at the downstream site were increased soft tissue mass (and gonad index), inflammation, and Vtg proteins in male mussels as determined by a novel immunostaining methodology. The rainfall overflow sites had no effects on these markers, but were specifically associated with reduced Vtg proteins in females, dopamine (Dop), gonad lipids, and DNA strand breaks, with increased metallothioneins. In conclusion, the observed feminizing effects of municipal effluent were not additionally observed in mussels caged at rainfall overflow sites, although the latter exhibited a different pattern of toxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative toxicity of urban wastewater and rainfall overflow in caged freshwater mussel Elliptio complanata

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It is noteworthy that the gills of D. chilensis accumulated iron but not Cu 2+ and showed increased GST activity after 30 and 60 days of exposure to a polluted medium with elevated concentrations of both metals (Yusseppone et al, 2020). Together with our results, this finding suggests that the gills of this species respond to Cu 2+ with the induction of GST and CAT activity but do not accumulate Cu 2+ , independently of the route and time of exposure.…”

Section: Discussionmentioning

confidence: 99%

“…There are reports on the decline of D. chilensis populations due to eutrophication and contamination in both countries (see Parada & Peredo, 2005; Parada et al, 2009; Rocchetta et al, 2014). In Argentina, this species is abundant in pristine lakes protected in national parks, but its distribution extends to areas contaminated by sewage effluents and leachates from garbage dumps (Sabatini, Rocchetta, Luquet et al, 2011; Yusseppone et al, 2019, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Copper and Escherichia coli Challenge on the Immune Response and Gill Oxidative Balance in the Freshwater Mussel Diplodon chilensis

Castro

Bianchi

Felici

et al. 2022

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Copper is a water and sediment pollutant that can be biomagnified by phytoplankton, and it often co‐occurs with fecal bacteria. We addressed the combined effects of copper and Escherichia coli on the immune response and gill oxidative balance of the freshwater mussel Diplodon chilensis. Bivalves were sorted into four groups fed with 1) control algae, 2) bacteria (E. coli), 3) copper‐enriched algae (Cu2+) algae, and 4) copper‐enriched algae followed by bacteria (Cu2+ + E. coli). Cellular and humoral immune and cytotoxic variables were analyzed in hemolymph, and detoxifying/antioxidant enzyme activities (glutathione S‐transferase [GST] and catalase [CAT]) and lipid peroxidation (thiobarbituric acid reactive substances [TBARS]) were studied in gill tissue. The total hemocyte number increased after Cu2+ exposure, independently of the E. coli challenge. The proportion of hyalinocytes significantly diminished in the E. coli and Cu2+ groups but not in Cu2+ + E. coli groups; granulocytes significantly increased with E. coli but not with Cu2+ + E. coli treatments. Phagocytic activity was higher in all treatments than in control mussels. Acid phosphatase activity was increased by E. coli and inhibited by Cu2+ and Cu2+ + E. coli. Both E. coli and Cu2+ but not Cu2+ + E. coli augmented alkaline phosphatase activity. The Cu2+ and Cu2+ + E. coli treatments reduced the lysosomal membrane stability and cell viability. Humoral bacteriolytic and phenol oxidase activities were not affected by any treatment. The Cu2+ treatment induced gill CAT and GST activities and increased TBARS levels. The Cu2+ + E. coli treatment reversed this CAT and GST stimulation and increased the Cu2+ effect on TBARS. Dietary Cu2+ affects bivalves' immunological and oxidative status and impairs defensive responses against bacteria. In turn, E. coli potentiates the gill oxidative effects of Cu2+. Environ Toxicol Chem 2023;42:154–165. © 2022 SETAC

show abstract

Oxidative stress in the bivalve Diplodon chilensis under direct and dietary glyphosate-based formulation exposure

Iummato,

Sabatini,

Rocchetta

et al. 2024

Environ Sci Pollut Res

View full text Add to dashboard Cite

In situ experiment to evaluate biochemical responses in the freshwater mussel Diplodon chilensis under anthropogenic eutrophication conditions

Cited by 7 publications

References 72 publications

Comparative toxicity of urban wastewater and rainfall overflow in caged freshwater mussel Elliptio complanata

Comparative toxicity of urban wastewater and rainfall overflow in caged freshwater mussel Elliptio complanata

Effects of Dietary Copper and Escherichia coli Challenge on the Immune Response and Gill Oxidative Balance in the Freshwater Mussel Diplodon chilensis

Oxidative stress in the bivalve Diplodon chilensis under direct and dietary glyphosate-based formulation exposure

Contact Info

Product

Resources

About