In situ evaluation of the remineralizing capacity of pit and fissure sealants containing amorphous calcium phosphate and/or fluoride

Silva, Kélio Garcia; Pedrini, Denise; Delbem, Alberto Carlos Botazzo; Ferreira, Lilian; Cannon, Mark

doi:10.3109/00016350903260264

Cited by 48 publications

(47 citation statements)

References 24 publications

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“…Artificial caries lesions were created by immersing each enamel block in 24 mL of demineralizing solution (1.3 mmol/L Ca, 0.78 mmol/L P, 0.05 mol/L acetate buffer, 0.03 µgF/mL, pH 5.0) at 37°C for 16 hours [20]. This method produces a subsurface enamel demineralization without surface erosion [21]. Surface microhardness was measured again by another three indentations created right underneath the initial ones.…”

Section: Methodsmentioning

confidence: 99%

Enamel Formation Genes Influence Enamel Microhardness Before and After Cariogenic Challenge

Shimizu

Deeley

et al. 2012

PLoS ONE

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There is evidence for a genetic component in caries susceptibility, and studies in humans have suggested that variation in enamel formation genes may contribute to caries. For the present study, we used DNA samples collected from 1,831 individuals from various population data sets. Single nucleotide polymorphism markers were genotyped in selected genes (ameloblastin, amelogenin, enamelin, tuftelin, and tuftelin interacting protein 11) that influence enamel formation. Allele and genotype frequencies were compared between groups with distinct caries experience. Associations with caries experience can be detected but they are not necessarily replicated in all population groups and the most expressive results was for a marker in AMELX (p = 0.0007). To help interpret these results, we evaluated if enamel microhardness changes under simulated cariogenic challenges are associated with genetic variations in these same genes. After creating an artificial caries lesion, associations could be seen between genetic variation in TUFT1 (p = 0.006) and TUIP11 (p = 0.0006) with enamel microhardness. Our results suggest that the influence of genetic variation of enamel formation genes may influence the dynamic interactions between the enamel surface and the oral cavity.

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Section: Methodsmentioning

confidence: 99%

Enamel Formation Genes Influence Enamel Microhardness Before and After Cariogenic Challenge

Shimizu

Deeley

et al. 2012

PLoS ONE

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“…Resin‐based materials with ion‐releasing capabilities have been investigated since the early years of resin composites use . Recently, luting and restorative composites, as well as liners and sealants containing calcium phosphates became available for clinical use . Manufacturers claim that these materials can provide protection against caries by offering an extra source of calcium and phosphate ions to create a supersaturated environment that would favor remineralization.…”

Section: Introductionmentioning

confidence: 99%

Calcium and phosphate release from resin‐based materials containing different calcium orthophosphate nanoparticles

et al. 2015

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The study compared ion release from resin-based materials containing calcium orthophosphates. Amorphous calcium phosphate (ACP), dicalcium phosphate anhydrous (DCPA), dicalcium phosphate dihydrate (DCPD), and tricalcium phosphate (β-TCP) nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), and surface area (nitrogen adsorption isotherms, BET method). Nanoparticles were added to a dimethacrylate-based resin and materials were tested for degree of conversion (DC) and calcium/phosphate release up to 28 days under pH 5.5 and 7.0. Data were analyzed by ANOVA/Tukey test (alpha: 0.05).The crystallinity of DCPA, DCPD, and β-TCP were confirmed, as well as the ACP amorphous nature. DCPD and β-TCP presented larger agglomerates than DCPA and ACP. The surface area of ACP was 5-11 times higher than those of the other nanoparticles. Materials showed similar DC. The material containing ACP released significantly more ions than the others, which released similar amounts of calcium and, in most cases, phosphate. Ion release was not affected by pH. Calcium release decreased between 7 and 21 days, while phosphate levels remained constant after 14 days. In conclusion, ACP higher ion release can be ascribed to its high surface area. DCPA, DCPD, and β-TCP had similar performances as ion-releasing fillers.

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“…For instance, a number of studies 4,5) have investigated the microleakage and penetrability of pit and fissure sealants containing ACP in permanent teeth and emphasized the importance of this type of sealant. Another study indicated that; PFSs containing ACP may promote remineralization of artificially induced carious lesion on smooth enamel surfaces 6) . Acid etching of the enamel is one of the most common and important stages in resin-based PFS application in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Effect of acid etching and different Er:YAG laser procedures on microleakage of three different fissure sealants in primary teeth after aging

et al. 2013

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The purpose of this study was to evaluate the effects of different surface conditioning procedures on microleakage and unfilled area proportions of three fissure sealants(Aegis, Helioseal and Helioseal F) in primary molar teeth. One hundred and fifty teeth were randomly divided into five main groups according to surface conditioning procedures. Main groups were; acid-etching(Group A); laser 3.75 W(Group B) and 5 W(Group D); acid-etching combined with laser 3.75 W(Group C) and 5 W(Group E). Samples were aged by thermocycling and water bath. Afterwards, they were immersed in basic fuchsin solution for 24 h and bucco-lingual sections were obtained from each tooth. Digital images were taken using a stereo-microscope and the microleakage and unfilled area proportions were assessed by using a software system. Consequently, Aegis FS containing amorphous calcium phosphate was found to exhibit the lowest microleakage in all surface conditioning procedures. Unfilled areas were not detected in Groups C and E of all FSs.

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In situ evaluation of the remineralizing capacity of pit and fissure sealants containing amorphous calcium phosphate and/or fluoride

Abstract: The pit and fissure sealants containing ACP were able to promote remineralization of artificially induced carious lesions on smooth enamel surfaces.

Cited by 48 publications

References 24 publications

Enamel Formation Genes Influence Enamel Microhardness Before and After Cariogenic Challenge

Enamel Formation Genes Influence Enamel Microhardness Before and After Cariogenic Challenge

Calcium and phosphate release from resin‐based materials containing different calcium orthophosphate nanoparticles

Effect of acid etching and different Er:YAG laser procedures on microleakage of three different fissure sealants in primary teeth after aging

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