In situ evaluation of breast cancer cell growth with 3D ATR-FTIR spectroscopy

Yang, Wenyue; Xiao, Xilin; Tan, Jun; Cai, Qingyun

doi:10.1016/j.vibspec.2008.04.016

Cited by 26 publications

(34 citation statements)

References 31 publications

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“…Well known rapid collection, reliable data and a powerful ability to structure elucidation of Fourier-transform infrared (FTIR) spectroscopy technology [3] added to the need for a rapid diagnosis versus the traditional biopsy has potentiated infrared imaging as a way for a new type of analysis based on optical examination. In addition, it is more objective than conventional colour methods, since it is possible "to read" the biochemical changes instead of an approach based on morphological changes.…”

Section: Introductionmentioning

confidence: 99%

A decade (2004 – 2014) of FTIR prostate cancer spectroscopy studies: An overview of recent advancements

Siqueira

Lima

2016

TrAC Trends in Analytical Chemistry

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Section: Introductionmentioning

confidence: 99%

A decade (2004 – 2014) of FTIR prostate cancer spectroscopy studies: An overview of recent advancements

Siqueira

Lima

2016

TrAC Trends in Analytical Chemistry

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“…12 It has been found to inhibit the growth of human tumor resulting in lower creatine levels in cancerous cells. 35 There is a shift that is also noticed at the 2854 cm −1 peak which is assigned to the symmetric (V s stretching vibrations of the -CH 2 groups of lipids within the cell membrane. 27 36 49 As reported by Huleihel, 50 shifts by several wavenumbers in the range of 2800-3000 cm −1 have been noticed in going from cancerous (MCF-7) to noncancerous breast cell lines as well as peak intensity increases.…”

Section: Atr-ftir Spectroscopy Methods To Differentiate Between Normalmentioning

confidence: 93%

“…Of all the peaks in range-1, the absorbances for all seem to decrease in small amounts for each peak. According to the paper, 35 wavenumber shifts were noticed for the MCF-7 cell line versus a normal cell line in the range of 1083-1639 cm −1 . These shifts were to a lower wavenumber towards the cancerous cell line at the 1084, 1240, 1456 cm −1 peaks.…”

Section: Atr-ftir Spectroscopy Methods To Differentiate Between Normalmentioning

confidence: 98%

“…34 The cells were trypsinized with 0.25% tryspsin and 0.02% EDTA and then centrifuged for 8 min at 2000 rpm. 35 Once the centrifugation was accomplished, the supernatant was drawn off and HBSS (500 L) was added to each tube and aspirated. 25 L of the resulting cell suspension was used from each vial for the Trypan-blue staining method for viability.…”

Section: Cell Culturementioning

confidence: 99%

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Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy Method to Differentiate Between Normal and Cancerous Breast Cells

Lane

See²

2012

j nanosci nanotechnol

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Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) is used to find the structural differences between cancerous breast cells (MCF-7 line) and normal breast cells (MCF-12F line). Gold nanoparticles were prepared and the hydrodynamic diameter of the gold nanoparticles found to be 38.45 nm. The Gold nanoparticles were exposed to both MCF-7 and MCF-12F cells from lower to higher concentrations. Spectroscopic studies founds nanoparticles were within the cells, and increasing the nanoparticles concentration inside the cells also resulted in sharper IR peaks as a result of localized surface Plasmon resonance. Asymmetric and symmetric stretching and bending vibrations between phosphate, COO-, CH2 groups were found to give negative shifts in wavenumbers and a decrease in peak intensities when going from noncancerous to cancerous cells. Cellular proteins produced peak assignments at the 1542 and 1644 cm(-1) wavenumbers which were attributed to the amide I and amide II bands of the polypeptide bond of proteins. Significant changes were found in the peak intensities between the cell lines in the spectrum range from 2854-2956 cm(-1). Results show that the concentration range of gold nanoparticles used in this research showed no significant changes in cell viability in either cell line. Therefore, we believe ATR-FTIR and gold nanotechnology can be at the forefront of cancer diagnosis for some time to come.

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“…In addition, MIR may be strongly absorbed by the phospholipids of cancer cells, thereby affecting cancer cell proliferation. Stretching bands of CH 2 and CH 3 in phospholipids are found at 3.4 and 3.5 mm, respectively (Yang et al, 2009). It is also possible that water molecules participate in photobiological functions during MIR irradiation, considering that the absorption spectrum of these molecules is mainly within the MIR spectral range (Cooper, 2000).…”

Section: Discussionmentioning

confidence: 99%

The effects of narrow-band middle infrared radiation in enhancing the antitumor activity of paclitaxel

Tsai

Sheu

Huang

et al. 2015

Electromagnetic Biology and Medicine

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Paclitaxel is used as an adjuvant to enhance the effectiveness of ionization radiation therapy; however, high-energy radiation often damages the healthy cells surrounding cancer cells. Low-energy, middle-infrared radiation (MIR) has been shown to prevent tissue damage, and recent studies have begun combining MIR with paclitaxel. However, the cytotoxic effects of this treatment combination remain unclear, and the mechanism underlying its effects on HeLa cells has yet to be elucidated. This study investigated the effectiveness of treating HeLa human cervical cancer cells with a combination of paclitaxel for 48 h in conjunction with narrow-band MIR from 3.0 to 5.0 μm. This combined treatment significantly inhibited the growth of HeLa cells. Specifically, results from Annexin V-FITC/PI apoptosis detection and cell mitochondrial membrane potential analyses revealed an increase in apoptotic cell death and a collapse of mitochondrial membrane potential. One possible mechanism underlying cellular apoptosis is an increase in oxidative stress. These preliminary findings provide evidence to support the combination of narrow-band MIR with paclitaxel as an alternative approach in the treatment of human cervical cancer.

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In situ evaluation of breast cancer cell growth with 3D ATR-FTIR spectroscopy

Cited by 26 publications

References 31 publications

A decade (2004 – 2014) of FTIR prostate cancer spectroscopy studies: An overview of recent advancements

A decade (2004 – 2014) of FTIR prostate cancer spectroscopy studies: An overview of recent advancements

Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy Method to Differentiate Between Normal and Cancerous Breast Cells

The effects of narrow-band middle infrared radiation in enhancing the antitumor activity of paclitaxel

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