“…Here, the expression of the TG6 protein was highest in the cortex, especially in the piriform cortex, the lateral septum, the olfactory tubercle and the shell of the nucleus accumbens (Figure 2A; right panel). TG6 expression was specifically localized to neurons, but not to astrocytes or microglia, as we previously confirmed using the NeuN-, Iba1-and GFAP-specific antibodies [45]. Furthermore, the reaction products of TGs, the N-ε-(γ-glutamyl)-lysine isopeptide bonds (GGEL) (Dab-Ni, black staining), could also be found in neurons next to astrocytes (Figure 2B), which appeared in close proximity to TG6 (DAB, brown staining) positive neurons (Figure 2B; arrowheads).…”