In Situ Encapsulation of Cellulase in a Novel Mesoporous Metal–Organic Framework

Zheng, Suhua; Tao, Juan; Wang, Xiaoji

doi:10.1007/s10562-021-03672-y

Cited by 13 publications

(5 citation statements)

References 38 publications

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“…Only when the MOF can be obtained in aqueous media under mild pH and temperature conditions can this approach be addressed [32]. Zeolitic imidazole frameworks (ZIFs) formed by the metal ions Zn 2+ or Co 2+ can be synthesized quickly and under biocompatible conditions [2], and therefore many reports have described one-pot immobilization of different enzymes, like cellulase [33] or catalase [34], among others, on ZIF-8. Apart from ZIF-8, not many MOFs can be prepared under such mild conditions, mainly due to the very low solubility of organic linkers in water.…”

Section: The Origins and Rising Dominance Of The Enzyme-supporting Mofmentioning

confidence: 99%

Sustainable One-Pot Immobilization of Enzymes in/on Metal-Organic Framework Materials

et al. 2021

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The industrial use of enzymes generally necessitates their immobilization onto solid supports. The well-known high affinity of enzymes for metal-organic framework (MOF) materials, together with the great versatility of MOFs in terms of structure, composition, functionalization and synthetic approaches, has led the scientific community to develop very different strategies for the immobilization of enzymes in/on MOFs. This review focuses on one of these strategies, namely, the one-pot enzyme immobilization within sustainable MOFs, which is particularly enticing as the resultant biocomposite Enzyme@MOFs have the potential to be: (i) prepared in situ, that is, in just one step; (ii) may be synthesized under sustainable conditions: with water as the sole solvent at room temperature with moderate pHs, etc.; (iii) are able to retain high enzyme loading; (iv) have negligible protein leaching; and (v) give enzymatic activities approaching that given by the corresponding free enzymes. Moreover, this methodology seems to be near-universal, as success has been achieved with different MOFs, with different enzymes and for different applications. So far, the metal ions forming the MOF materials have been chosen according to their low price, low toxicity and, of course, their possibility for generating MOFs at room temperature in water, in order to close the cycle of economic, environmental and energy sustainability in the synthesis, application and disposal life cycle.

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Section: The Origins and Rising Dominance Of The Enzyme-supporting Mofmentioning

confidence: 99%

Sustainable One-Pot Immobilization of Enzymes in/on Metal-Organic Framework Materials

et al. 2021

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“…An addition of 4.6 nm mesopores in microporous Zr-MOF obtained by biomineralization with dextran as sacrificial template allows better entrapment of cellulase within the material, improving loading capacity and stability of immobilized cellulase [ 58 ]. Mesoporous Zn-based MOFs were also utilized for cellulase encapsulation by simultaneous precipitation of the MOF precursors and cellulase ( Figure 2 ) [ 59 ]. This significantly enhanced the cellulase loading and created structural defects during MOF formation (large pores), assisting in mass transfer and increasing enzymic activity.…”

Section: Methods Of Cellulase Immobilizationmentioning

confidence: 99%

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing

2022

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Nanobiocatalysts, i.e., enzymes immobilized on nanostructured supports, received considerable attention because they are potential remedies to overcome shortcomings of traditional biocatalysts, such as low efficiency of mass transfer, instability during catalytic reactions, and possible deactivation. In this short review, we will analyze major aspects of immobilization of cellulase—an enzyme for cellulosic biomass waste processing—on nanostructured supports. Such supports provide high surface areas, increased enzyme loading, and a beneficial environment to enhance cellulase performance and its stability, leading to nanobiocatalysts for obtaining biofuels and value-added chemicals. Here, we will discuss such nanostructured supports as carbon nanotubes, polymer nanoparticles (NPs), nanohydrogels, nanofibers, silica NPs, hierarchical porous materials, magnetic NPs and their nanohybrids, based on publications of the last five years. The use of magnetic NPs is especially favorable due to easy separation and the nanobiocatalyst recovery for a repeated use. This review will discuss methods for cellulase immobilization, morphology of nanostructured supports, multienzyme systems as well as factors influencing the enzyme activity to achieve the highest conversion of cellulosic biowaste into fermentable sugars. We believe this review will allow for an enhanced understanding of such nanobiocatalysts and processes, allowing for the best solutions to major problems of sustainable biorefinery.

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“…The disadvantage of this method is that it is difficult to use MOF's rich pore structure to realize the surface immobilization method. For the in situ encapsulation method, cellulase is added during the formation of MOF and then encapsulated in the framework of MOF through coprecipitation [30]. The preparation of MOF must be conducted under mild conditions.…”

Section: Conversion Of Cellulose By Mof-immobilized Enzymementioning

confidence: 99%

Recent Progress on Conversion of Lignocellulosic Biomass by MOF-Immobilized Enzyme

Tao,

Song,

2024

Polymers

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The enzyme catalysis conversion of lignocellulosic biomass into valuable chemicals and fuels showed a bright outlook for replacing fossil resources. However, the high cost and easy deactivation of free enzymes restrict the conversion process. Immobilization of enzymes in metal–organic frameworks (MOFs) is one of the most promising strategies due to MOF materials’ tunable building units, multiple pore structures, and excellent biocompatibility. Also, MOFs are ideal support materials and could enhance the stability and reusability of enzymes. In this paper, recent progress on the conversion of cellulose, hemicellulose, and lignin by MOF-immobilized enzymes is extensively reviewed. This paper focuses on the immobilized enzyme performances and enzymatic mechanism. Finally, the challenges of the conversion of lignocellulosic biomass by MOF-immobilized enzyme are discussed.

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In Situ Encapsulation of Cellulase in a Novel Mesoporous Metal–Organic Framework

Cited by 13 publications

References 38 publications

Sustainable One-Pot Immobilization of Enzymes in/on Metal-Organic Framework Materials

Sustainable One-Pot Immobilization of Enzymes in/on Metal-Organic Framework Materials

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing

Recent Progress on Conversion of Lignocellulosic Biomass by MOF-Immobilized Enzyme

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