In situ electrochemical analysis of alkaline phosphatase activity in 3D cell cultures

Caviglia, Claudia; Carletto, Rodrigo Pimentel; Roni, Stefania De; Hassan, Yasmin; Hemanth, Suhith; Dufva, Martin; Keller, Stephan Sylvest

doi:10.1016/j.electacta.2020.136951

Cited by 18 publications

(13 citation statements)

References 40 publications

(57 reference statements)

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“…Under the optimized conditions mentioned above, the sensitivity of the PtμE/Au aptasensor against CEA was investigated by measuring SWV starting at the concentration of 1.0 × 10 –7 g/ml and diluting the CEA solution by a factor of 10 each time through PBS solution until a limit of detection (LOD) could be detected, and SWVs were recorded in each concentration three times ( Taylor et al, 2019 ; Hannah et al, 2020 ). A linear relationship between the ∆I and each [CEA] was observed ( Caviglia et al, 2020 ). The PtμEs/Au aptasensor exhibits a linear response toward CEA in the concentration range of 10 –11 -10 –7 g/ml (S = 5.5 nA/dec, R 2 = 0.999), and the LOD is 7.7 × 10 –12 g/ml, which is calculated according to LOD = 3σ/b, where σ is the standard deviation of “ n ”, the number of SWV in blank solution, and b represents the slope of the calibration plot ( Figure 5 ) ( Shah et al, 2019 ; Gupta et al, 2020 ).…”

Section: Resultsmentioning

confidence: 96%

Development of Carcinoembryonic Antigen Rapid Detection System Based on Platinum Microelectrode

Zhai

et al. 2022

Front. Chem.

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Rapid and highly sensitive detection of carcinoembryonic antigen (CEA) in blood could effectively improve the diagnostic sensitivity of colorectal cancer. In this work, a platinum microelectrode (PtμE) modified with gold nanoparticles was developed as a microsensor for the detection of CEA. As the recognition element, a CEA aptamer modified with sulfhydryl could be conjugated onto the surface of the PtμEs/Au. The quantitative analysis of the concentration of CEA [CEA] by the prepared PtμEs/Au aptasensor was carried out through square wave voltammetry. Under the optimized conditions, the PtμEs/Au aptasensor exhibits a linear response toward [CEA] in the range of 1.0 × 10–11—1.0 × 10–7 g/ml (S = 5.5 nA/dec, R2 = 0.999), and the detection limit is 7.7 × 10–12 g/ml. The PtμEs/Au aptasensor also has good selectivity against other types of proteins existing in blood. The availability of the developed assay toward [CEA] in blood samples was investigated, and the results agreed well with those obtained through electrochemiluminescence provided by the hospital, and the volume of the blood sample for detection is only 20 μl. Herein, the proposed detection system could be used for the quantitative analysis of CEA in blood, with the advantages of high sensitivity, short time, and low cost. Moreover, the PtμEs/Au aptasensor has a potential application in clinical diagnosis.

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Section: Resultsmentioning

confidence: 96%

Development of Carcinoembryonic Antigen Rapid Detection System Based on Platinum Microelectrode

Zhai

et al. 2022

Front. Chem.

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“…Importantly, polymers are the most common materials investigated as carbon precursors for biological application. Their unique polymer features allow the fabrication of microscale and nanoscale scaffolds with customisable pattern alignment similar to the ECM of cells [ 88 , 89 ]. The negative photoresist SU-8 polymer has been extensively used to produce carbon-based scaffolds for biological application and have shown to be a promising material in tissue engineering.…”

Section: Carbon Precursors As Scaffold Biomaterials For Scs Applicationsmentioning

confidence: 99%

Modelling of Stem Cells Microenvironment Using Carbon-Based Scaffold for Tissue Engineering Application—A Review

2021

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A scaffold is a crucial biological substitute designed to aid the treatment of damaged tissue caused by trauma and disease. Various scaffolds are developed with different materials, known as biomaterials, and have shown to be a potential tool to facilitate in vitro cell growth, proliferation, and differentiation. Among the materials studied, carbon materials are potential biomaterials that can be used to develop scaffolds for cell growth. Recently, many researchers have attempted to build a scaffold following the origin of the tissue cell by mimicking the pattern of their extracellular matrix (ECM). In addition, extensive studies were performed on the various parameters that could influence cell behaviour. Previous studies have shown that various factors should be considered in scaffold production, including the porosity, pore size, topography, mechanical properties, wettability, and electroconductivity, which are essential in facilitating cellular response on the scaffold. These interferential factors will help determine the appropriate architecture of the carbon-based scaffold, influencing stem cell (SC) response. Hence, this paper reviews the potential of carbon as a biomaterial for scaffold development. This paper also discusses several crucial factors that can influence the feasibility of the carbon-based scaffold architecture in supporting the efficacy and viability of SCs.

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“…The flat 2D working electrode (2DWE) had a geometrical shape of a circle with a diameter of 4 mm and a surface area of 12.6 mm 2 . The 3D working electrode (3DWE) consisted of additional 284 micropillars with a height of 225 μm and a diameter of 68 μm patterned on top of the 2DWE resulting in a total geometrical surface area of 26.2 mm 2 [55].…”

Section: Pyrolytic Carbon Electrode Fabricationmentioning

confidence: 99%

“…3D pyrolytic carbon electrodes have been fabricated using cleanroom based microfabrication processes, allowing for excellent tailoring ability of electrode geometry, configuration and properties for a specific application [52]. In several studies, enhanced sensitivity for electrochemical biosensing with 3D pyrolytic carbon electrodes has been demonstrated [53][54][55].…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Peroxide Detection Using Prussian Blue‐modified 3D Pyrolytic Carbon Microelectrodes

et al. 2021

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A highly sensitive amperometric Prussian bluebased hydrogen peroxide sensor was developed using 3D pyrolytic carbon microelectrodes. A 3D printed multielectrode electrochemical cell enabled simultaneous highly reproducible Prussian blue modification on multiple carbon electrodes. The effect of oxygen plasma pretreatment and deposition time on Prussian blue electro-deposition was studied. The amperometric response of 2D and 3D sensors to the addition of hydrogen peroxide in μM and sub-μM concentrations in phosphate buffer was investigated. A high sensitivity comparable to flow injection systems and a detection limit of 0.16 μM was demonstrated with 3D pyrolytic carbon microelectrodes at stirred batch condition

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In situ electrochemical analysis of alkaline phosphatase activity in 3D cell cultures

Cited by 18 publications

References 40 publications

Development of Carcinoembryonic Antigen Rapid Detection System Based on Platinum Microelectrode

Development of Carcinoembryonic Antigen Rapid Detection System Based on Platinum Microelectrode

Modelling of Stem Cells Microenvironment Using Carbon-Based Scaffold for Tissue Engineering Application—A Review

Hydrogen Peroxide Detection Using Prussian Blue‐modified 3D Pyrolytic Carbon Microelectrodes

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