In situ electrical modulation and monitoring of nanoporous gold morphology

Dorofeeva, Tatiana S.; Şeker, Erkin

doi:10.1039/c6nr07237b

Cited by 13 publications

(9 citation statements)

References 30 publications

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“…To understand the effect of pore morphology on electrochemical sensing phenomena at the nanolevel, a group of np-Au samples (unannealed np-Au) was thermally annealed, as routinely performed. 44 The median pore radii of unannealed and annealed np-Au electrodes were 24 and 56 nm, respectively, determined by analysis of SEM images (Figure 1a,b). Interconnected noncolumnar pores and the resulting "funnel-like" constrictions are evident in the crosssection images of the two morphologies (Figure 1c,d).…”

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confidence: 99%

Anomalous Trends in Nucleic Acid-Based Electrochemical Biosensors with Nanoporous Gold Electrodes

2019

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Molecular diagnostics have significantly advanced the early detection of diseases, where electrochemical sensing of biomarkers has shown considerable promise. For a nucleic acid-based electrochemical sensor with signal-off behavior, the performance is evaluated by percent signal suppression (% ss), which indicates the change in current after hybridization. The % ss is generally due to more redox molecules (e.g., methylene blue) associating with the probe DNA bases in the single-strand form than the double-strand form upon hybridization with the target nucleic acid. Nanostructured electrodes generally enhance electrochemical sensor performance via several mechanisms, including increased number of capture probes per electrode volume and unique nanoscale transport phenomena. Here, we employ nanoporous gold (np-Au) as a model electrode material to study the influence of probe immobilization solution concentration on sensor performance and the underlying mechanisms. Unlike planar gold (pl-Au) electrodes, where % ss reaches a steady state with increasing concentration of the grafting solution, the % ss displays peak performance at certain grafting solution concentrations followed by rapid deterioration and reversal of the % ss polarity, suggesting an unexpected case of increased charge transfer upon hybridization. Fluorometric assessments of electrochemically desorbed nucleic acids for different electrode morphologies reveal that a significant amount of DNA molecules (unhybridized and hybridized) remain within the nanopores posthybridization. Analysis of electrochemical signals (e.g., square wave voltammogram shape) suggests that the large unbound nucleic acid concentration may be altering the modes of methylene blue interaction with the nucleic acids and charge transfer to the electrode surfaces.

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Anomalous Trends in Nucleic Acid-Based Electrochemical Biosensors with Nanoporous Gold Electrodes

2019

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“…In order to achieve a better antifouling performance, the pore size need to be decreased to the nanoscale (see Figure 5b) [69]. Seker's group has employed nanoporous Au electrodes to construct low fouling DNA sensors for electrochemical detection of target DNAs in biofluids with a high sensitivity and low limit of detection (LOD) [70][71][72][73][74]. Alex M. Downs et al have showed that the porous Au microelectrode has the ability of detecting aptamers in the living body.…”

Section: Porous Coatingsmentioning

confidence: 99%

An Overview of Antifouling Strategies for Electrochemical Analysis

Zhou

Zhu

et al. 2021

Electroanalysis

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Fouling is a key issue which limits the practical applications of electrochemical sensors (ECSens) in both in vitro and in vivo analysis. The analytical sensitivity, limit of detection (LOD), selectivity, reproducibility and life-time of ECSens are all greatly affected by fouling. Although numerous methods and materials have been developed to minimize the fouling effect in past several decades, not all of them are suitable for constructing antifouling ECSens. In this paper we present an overview of surface antifouling strategies for ECSens. We begin with a short summary of sources and impacts of fouling under both in vitro and in vivo environments. Then the current surface antifouling strategies for ECSens are discussed, including self-assembled monolayers (SAMs), antifouling polymers, porous coating, in-situ electrochemical and photocatalytic cleaning. The antifouling mechanisms, advantages and challenges of each strategy are briefly summarized. The article ends up with a short conclusion and perspectives. We hope this article is helpful for constructing and designing ECSens with both outstanding antifouling performance and electrochemical activity for electrochemical analysis.

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“…Various kinds of nanoporous surface have been fabricated in previous studies [33][34][35][36][37]. The high density and uniformity of Au nanoporous substrates make them suitable as SERS substrates.…”

Section: Fabrication Of the Au Nanoporous Sers Substratementioning

confidence: 99%

Mutated Human P-Selectin Glycoprotein Ligand-1 and Viral Protein-1 of Enterovirus 71 Interactions on Au Nanoplasmonic Substrate for Specific Recognition by Surface-Enhanced Raman Spectroscopy

et al. 2020

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Protein tyrosine sulfation is a common post-translational modification that stimulates intercellular or extracellular protein-protein interactions and is responsible for various important biological processes, including coagulation, inflammation, and virus infections. Recently, human P-selectin glycoprotein ligand-1 (PSGL-1) has been shown to serve as a functional receptor for enterovirus 71 (EV71). It has been proposed that the capsid viral protein VP1 of EV71 is directly involved in this specific interaction with sulfated or mutated PSGL-1. Surface-enhanced Raman spectroscopy (SERS) is used to distinguish PSGL-1 and VP1 interactions on an Au nanoporous substrate and identify specific VP1 interaction positions of tyrosine residue sites (46, 48, and 51). The three tyrosine sites in PSGL-1 were replaced by phenylalanine (F), as determined using SERS. A strong phenylalanine SERS signal was obtained in three regions of the mutated protein on the nanoporous substrate. The mutated protein positions at (51F) and (48F, 51F) produced a strong SERS peak at 1599–1666 cm−1, which could be related to a binding with the mutated protein and anti-sulfotyrosine interactions on the nanoporous substrate. A strong SERS effect of the mutated protein and VP1 interactions appeared at (48F), (51F), and (46F, 48F). In these positions, there was less interaction with VP1, as indicated by a strong phenylalanine signal from the mutated protein.

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In situ electrical modulation and monitoring of nanoporous gold morphology

Cited by 13 publications

References 30 publications

Anomalous Trends in Nucleic Acid-Based Electrochemical Biosensors with Nanoporous Gold Electrodes

Anomalous Trends in Nucleic Acid-Based Electrochemical Biosensors with Nanoporous Gold Electrodes

An Overview of Antifouling Strategies for Electrochemical Analysis

Mutated Human P-Selectin Glycoprotein Ligand-1 and Viral Protein-1 of Enterovirus 71 Interactions on Au Nanoplasmonic Substrate for Specific Recognition by Surface-Enhanced Raman Spectroscopy

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