In situ dose amplification by apoptosis-targeted drug delivery

Wang, Kai; Na, Moon Hee; Hoffman, Allan S.; Shim, Gayong; Han, Su Eun; Oh, Yu‐Kyoung; Kwon, Ick Chan; Kim, In San; Lee, Byung Heon

doi:10.1016/j.jconrel.2011.06.043

Cited by 25 publications

(24 citation statements)

References 22 publications

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“…In good agreement with these studies, Wang et al reported tumor targeting of doxorubicin-loaded liposomes harboring the histone H1-specific peptide ApoPep-1 [138]. This peptide is selectively presented at the surface of tumor cells due to spontaneous apoptosis in avascular tumors.…”

Section: Stealth Targeted Liposomesmentioning

confidence: 57%

Recent Trends in Multifunctional Liposomal Nanocarriers for Enhanced Tumor Targeting

Perche

Torchilin

2013

Journal of Drug Delivery

195

144

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Liposomes are delivery systems that have been used to formulate a vast variety of therapeutic and imaging agents for the past several decades. They have significant advantages over their free forms in terms of pharmacokinetics, sensitivity for cancer diagnosis and therapeutic efficacy. The multifactorial nature of cancer and the complex physiology of the tumor microenvironment require the development of multifunctional nanocarriers. Multifunctional liposomal nanocarriers should combine long blood circulation to improve pharmacokinetics of the loaded agent and selective distribution to the tumor lesion relative to healthy tissues, remote-controlled or tumor stimuli-sensitive extravasation from blood at the tumor's vicinity, internalization motifs to move from tumor bounds and/or tumor intercellular space to the cytoplasm of cancer cells for effective tumor cell killing. This review will focus on current strategies used for cancer detection and therapy using liposomes with special attention to combination therapies.

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Section: Stealth Targeted Liposomesmentioning

confidence: 57%

Recent Trends in Multifunctional Liposomal Nanocarriers for Enhanced Tumor Targeting

Perche

Torchilin

2013

Journal of Drug Delivery

195

144

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“…During chemotherapy, tumor cells typically undergo apoptosis, which subsequently increases the levels of cell surface apoptosis biomarkers such as histone H1 and PS. Wang et al demonstrated an interesting theranostic strategy by preparing doxorubicin-loaded liposomes that were labeled with the histone H1 targeting peptide, Apopep-1 (207). The strategy is summarized schematically in Figure 6.…”

Section: Apoptosis Biomarkersmentioning

confidence: 99%

Biomarkers and Molecular Probes for Cell Death Imaging and Targeted Therapeutics

2012

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Cell death is a critically important biological process. Disruption of homeostasis, either by excessive or deficient cell death, is a hallmark of many pathological conditions. Recent research advances have greatly increased our molecular understanding of cell death and its role in a range of diseases and therapeutic treatments. Central to these ongoing research and clinical efforts is the need for imaging technologies that can locate and identify cell death in a wide array of in vitro and in vivo biomedical samples with varied spatiotemporal requirements. This review article summarizes community efforts over the past five years to identify useful biomarkers for dead and dying cells, and to develop molecular probes that target these biomarkers for optical, radionuclear, or magnetic resonance imaging. Apoptosis biomarkers are classified as either intracellular (caspase enzymes, mitochondrial membrane potential, cytosolic proteins) or extracellular (plasma membrane phospholipids, membrane potential, surface exposed histones). Necrosis, autophagy, and senescence biomarkers are described, as well as unexplored cell death biomarkers. The article discusses possible chemotherapeutic and theranostic strategies, and concludes with a summary of current challenges and expected eventual rewards of clinical cell death imaging.

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“…Since successful anticancer therapeutics such as chemo- and radiation-therapy rely on apoptosis, a robust apoptosis monitoring and screening method has the potential to accelerate the process of drug discovery and development. Various strategies based on different imaging modalities have been reported to image apoptotic processes in cells and in vivo, which are well summarized elsewhere [7-11]. …”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of caspase cascade activation in living cells

Zhu

Choi

et al. 2012

Journal of Controlled Release

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We introduce a simple, versatile and robust one-step technique that enables real-time imaging of multiple intracellular caspase activities in living cells without the need for complicated synthetic protocols. Conventional fluorogenic probes or recently reported activatable probes have been designed to target various proteases but are limited to extracellular molecules. Only a few have been applied to image intracellular proteases in living cells because most of these probes have limited cell-permeability. Our platform does not need complicated synthetic processes; instead it involves a straightforward peptide synthesis and a simple mixing step with a commercial transfection agent. The transfection agent efficiently delivered the highly quenched fluorogenic probes, comprised of distinctive pairs of dyes and quenchers, to the initiator caspase-8 and the effector caspase-3 in MDA-MB-435 cells, allowing dual-imaging of the activities of both caspases during the apoptotic process induced by TNF-related apoptosis induced ligand (TRAIL). With the combination of multiple fluorogenic probes, this simple platform can be applied to multiplexed imaging of selected intracellular proteases to study apoptotic processes in pathologies or for cell-based high throughput screening systems for drug discovery.

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In situ dose amplification by apoptosis-targeted drug delivery

Cited by 25 publications

References 22 publications

Recent Trends in Multifunctional Liposomal Nanocarriers for Enhanced Tumor Targeting

Recent Trends in Multifunctional Liposomal Nanocarriers for Enhanced Tumor Targeting

Biomarkers and Molecular Probes for Cell Death Imaging and Targeted Therapeutics

Real-time monitoring of caspase cascade activation in living cells

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