In situ DNA hybridization analysis of oral papillomas, leukoplakias, and carcinomas for human papillomavirus

Young, Stephen K.; Min, Kyung Whan

doi:10.1016/0030-4220(91)90282-h

Cited by 92 publications

(39 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The fact that studies on oral papillomas showed an HPVpositivity in 13-60%, predominantly HPV6 or 211, [44][45][46] makes the absence of HPV in the tonsillar papillomas in our series remarkable. The lack of HPV in these samples might be explained by elimination of the virus by the immune system with persistence of the lesions, or by the fact that other viruses, yet to be discovered, play an etiological role in the development of the tonsillar papillomas.…”

Section: Discussionmentioning

confidence: 45%

P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

Mooren

Gültekin

Straetmans

et al. 2013

Intl Journal of Cancer

View full text Add to dashboard Cite

Human papillomavirus (HPV) is a risk factor for the development of benign and malignant mucosal head and neck lesions. P16 INK4A is often used as a surrogate marker for HPV-infection, although there is still controversy with respect its reliability. Our aim was to determine if p16 INK4A overexpression can accurately predict both high-risk and low-risk-HPV-presence in (pre)-malignant and benign head and neck lesions. P16 INK4A immunohistochemistry was performed on paraffin-embedded tissue sections of 162 oropharyngeal squamous cell carcinomas (OPSCC), 14 tonsillar and 23 laryngeal dysplasias, and 20 tonsillar and 27 laryngeal papillomas. PCR, enzyme-immunoassay and FISH analysis were used to assess HPV-presence and type. Of the 162 OPSCC and 14 tonsillar dysplasias, 51 (31%) and 10 (71%) were HPV16-positive, respectively. All tonsillar papillomas were HPV-negative and four laryngeal dysplasias and 26 laryngeal papillomas were positive for HPV6 or 211. P16 INK4A immunohistochemistry revealed a strong nuclear and cytoplasmic staining in 50 out of 51 HPV16-positive and 5 out of 111 HPVnegative OPSCC (p < 0.0001) and in all HPV16-positive tonsillar dysplasias, whereas highly variable staining patterns were detected in the papillomas and laryngeal dysplasias, irrespective of the HPV-status. In addition, the latter lesions generally showed a higher nuclear than cytoplasmic p16 INK4A immunostaining intensity. In conclusion, our data show that strong nuclear and cytoplasmic p16 INK4A overexpression is a reliable surrogate indicator for HPV16 in OPSCC and (adjacent) dysplasias. For HPV6 or 211-positive and HPV-negative benign and premalignant lesions of the tonsil and larynx, however, p16 INK4A immunostaining is highly variable and cannot be recommended to predict HPV-presence.

show abstract

Section: Discussionmentioning

confidence: 45%

P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

Mooren

Gültekin

Straetmans

et al. 2013

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…16,17 However, there are conflicting data in the literature on the identification of HPV DNA by ISH techniques. Some investigators either failed to identify HPV DNA in none of oral cavity VC, 18 or identified HPV DNA in a very limited number of cases. [19][20][21] Perhaps these discrepancies relate to the difficulties in the interpretation of ISH studies.…”

Section: Discussionmentioning

confidence: 99%

Investigation of HPV Infection and P53 Expression in Laryngeal Verrucous Carcinoma

Tunçbilek¹,

Çallı²,

Öncel³

et al. 2010

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Ver ru co us car ci no ma is an un com mon, well-dif fe ren ti a ted va ri ant of squ a mo us cell car ci no ma that is re gar ded as a low gra de, lo cally in va si ve ma lig nant tu mor with ra re po ten ti al for dis tant me tas ta sis. Alt ho ugh the pat ho ge ne sis of ver ru co us car ci no ma is unk nown, many pos sib le ca u ses ha ve been pro po sed. The le a ding the o ri es inc lu de hu man pa pil lo ma vi rus (HPV) in fec ti on and che mi cal car ci noge ne sis. The aim of this study is to analy se the sta tus of the p53 ge ne and hu man pa pil lo ma vi rus (HPV) in fec ti on in ver ru co us car ci no ma of larynx, and to in ves ti ga te the cor re la ti on of p53 ex pres si on and HPV in fec ti on in ver ru co us car ci no ma of the larynx. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : A ret ros pec ti ve study has be en per for med bet we en the ye ars of 1994 and 2004, com pri sing 21 con se cu ti ve pa ti ents with ver ru co us carci no ma of larynx. De ta i led cli ni cal pa ra me ters ha ve al so be en re vi e wed. HPV iden ti fi ca ti on was ac complis hed with poly me ra se cha in re ac ti on. p53 im mu no his toc he mistry was per for med on 21 ver ru co us car ci no ma using poly clo nal se rum CM-1. R Re e s su ul lt ts s: : The HPV in fec ti on has be en de tec ted in in all of the twenty-one ca ses of ver ru co us car ci no mas. p53 im mu no his toc he mistry was per for med on 21 HPV conta i ning ver ru co us car ci no mas and sho wed the ex pres si on of p53 in 14 ca ses. No sta tis ti cally sig ni fi cant dif fe ren ces we re fo und bet we en p53 ex pres si on and sta ge. C Co on nc c l lu u s si i o on n: : The da ta in di ca te that HPV and the in cre a sed ex pres si on of p53 can co e xist in ver ru co us car ci no mas. Our study sug gests that verrucous carcinomas of the larynx may ari se thro ugh a still unk nown mec ha nism which ca u ses p53 inac ti va ti on or HPV in fec ti on.We think that p53 ove rex pres si on and on co ge nic HPV in fec ti on may co o pe ra te in the estab lish ment of ma lig nant cells in ver ru co us car ci no ma of the larynx. The in te rac ti on bet we en the HPV in fec ti on and cer ta in cel lu lar on co ge nes such as p53 ne eds furt her studies.K Ke ey y W Wo or rd ds s: : Car ci no ma, ver ru co us; larynx; p53 pro te in, hu man; pa pil lo ma vi rus in fec ti ons Ö ÖZ ZE ET T A Am ma aç ç: : Na dir me tas taz po tan si ye li bu lu nan dü şük de re ce li lo kal in va ziv ma lign tü mör ola rak bi linen ver rü köz kar si nom, sku a möz hüc re li kar si no mun iyi di fe ran si ye bir var yan tı dır. Ver rü köz kar si nomun pa to ge ne zi bi lin me se de ba zı ola sı ne den ler öne sü rül müş tür. Bun la rın ba şın da hu man pa pil lo ma vi rus enfeksiyonu ve kim ya sal kar si no ge nez gel mek te dir. Ça lış ma mı zın ama cı la rink sin ver rü köz kar sinom la rın da p53 gen ve hu man pa pil lo ma vi rus en fek si yo nu nun ana li zi ve p53 eks pres yo nu ile hu man papil lo ...

show abstract

“…In western countries, the detection rate of HPV in OELs varied, ranging from inconsistency to 54% [49, 50, 51, 52, 53]. The differences were considered to be geographical and/or methodological [54].…”

Section: Discussionmentioning

confidence: 99%

Pathological Features of Betel Quid-Related Oral Epithelial Lesions in Taiwan with Special Emphasis on the Tumor Progression and Human Papillomavirus Association

Chang¹,

Su²,

Tsai³

et al. 2002

Oncology

View full text Add to dashboard Cite

Betel quid (BQ) chewing has been a well-documented cause of oral epithelial lesions (OEL). Evolution from early hyperplastic lesions to the late or carcinomatous stage has been recognized. The pathobiological and molecular mechanism, however, remains to be elucidated. In this study, a total of 232 samples obtained from 153 cases of BQ-related OEL were retrospectively evaluated for the expression of p53 and bcl-2 in comparison with 26 cases of BQ-unrelated lesions (n = 29). The possible role of human papillomavirus (HPV) was also investigated. These BQ-related OELs included verrucous hyperplasia (VIH, n = 57, 24.6%), epithelial dysplasia (n = 23, 9.9%), verrucous carcinoma (VC, n = 5, 2.1%) and squamous cell carcinoma (SCC, n = 106, 45.7%). Fifty-four cases (35.3%) had multiple lesions. In comparison with the BQ-unrelated OELs, the characteristics of BQ-related OELs were a younger age, male predilection and multicentricity. In contrast to the tongue in BQ-unrelated OELs, the most common site for all types of BQ-related lesions was the buccal mucosa. Immunohistochemical studies of BQ-related lesions showed p53 staining in 30% of dysplasia and 38% of SCC, but a consistent absence in VH and VC. The cases with p53-positive SCC had a higher recurrence rate than p53-negative ones. Bcl-2 expression was negligible for all types of lesions. HPV-6/11 was detectable in 10% of dysplasia and 13% of SCC, but in neither VH nor VC. HPV-16/18, however, was consistently negative for all types of lesions. Our data suggest that p53, but not bcl-2, may play a role in tumor progression of BQ-related OELs, and that VH and VC are distinct and closely related histological lesions. The consistent absence of the malignant-type HPV in all BQ-related lesions suggests that HPV plays an insignificant role in the tumorigenesis of BQ-related oral cancers, although a cooperative role may exist between the benign-type HPV and BQ chewing.

show abstract

In situ DNA hybridization analysis of oral papillomas, leukoplakias, and carcinomas for human papillomavirus

Cited by 92 publications

References 14 publications

P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

Investigation of HPV Infection and P53 Expression in Laryngeal Verrucous Carcinoma

Pathological Features of Betel Quid-Related Oral Epithelial Lesions in Taiwan with Special Emphasis on the Tumor Progression and Human Papillomavirus Association

Contact Info

Product

Resources

About

In situ DNA hybridization analysis of oral papillomas, leukoplakias, and carcinomas for human papillomavirus

Cited by 92 publications

References 14 publications

P16INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

P16INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

Investigation of HPV Infection and P53 Expression in Laryngeal Verrucous Carcinoma

Pathological Features of Betel Quid-Related Oral Epithelial Lesions in Taiwan with Special Emphasis on the Tumor Progression and Human Papillomavirus Association

Contact Info

Product

Resources

About

P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias

P16^INK4A immunostaining is a strong indicator for high‐risk‐HPV‐associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low‐risk‐HPV‐infection in head and neck papillomas and laryngeal dysplasias