In situ detection of the protein corona in complex environments

Carril, Mónica; Padró, Daniel; Pino, Pablo del; Carrillo‐Carrión, Carolina; Gallego, Marta; Parak, Wolfgang J.

doi:10.1038/s41467-017-01826-4

Cited by 123 publications

(138 citation statements)

References 24 publications

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“…It should be noted, however, that our click chemistry approach relies on the presence of an azide group on an HC protein within the reach (~1.8 nm) of the DBCO moiety of SC proteins, thus likely underestimating the amount of SC proteins, particularly for low HC coverages. Nevertheless, the derived coverage ratio is consistent with previous studies claiming that the corona formed from serum consists essentially of a monolayer 2,30 . On this basis, we hypothesize that both HC and SC proteins co-exist within a loosely defined monolayer covering the nanoparticle surface, rather than in separate layers, and that SC proteins may dissociate from the surface during centrifugation.…”

Section: Fig 1 Spaac Click Chemistry Reaction and Characterizationsupporting

confidence: 90%

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Mohammad‐Beigi

Hayashi

Zeuthen

et al. 2020

Preprint

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The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identified a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins in the SC are revealed as modulators of nanoparticle-cell association, in spite of their short residence time. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

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Section: Fig 1 Spaac Click Chemistry Reaction and Characterizationsupporting

confidence: 90%

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Mohammad‐Beigi

Hayashi

Zeuthen

et al. 2020

Preprint

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“…Xu et al have used the hierarchical cluster (HCA) analysis to analyze the relation of physiochemical parameters determined protein corona and biological effects. The hydrodynamic radii, in vitro uptake and in vivo biodistribution of NPs seems to be indirectly driven by the protein corona, which is determined by the physiochemical properties of NPs, especially, including colloidal stability and surface chemistry …”

Section: Strategies Of Exploiting Protein Corona–nanoparticle Interacmentioning

confidence: 99%

The Crown and the Scepter: Roles of the Protein Corona in Nanomedicine

2018

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Engineering nanomaterials are increasingly considered promising and powerful biomedical tools or devices for imaging, drug delivery, and cancer therapies, but few nanomaterials have been tested in clinical trials. This wide gap between bench discoveries and clinical application is mainly due to the limited understanding of the biological identity of nanomaterials. When they are exposed to the human body, nanoparticles inevitably interact with bodily fluids and thereby adsorb hundreds of biomolecules. A “biomolecular corona” forms on the surface of nanomaterials and confers a new biological identity for NPs, which determines the following biological events: cellular uptake, immune response, biodistribution, clearance, and toxicity. A deep and thorough understanding of the biological effects triggered by the protein corona in vivo will speed up their translation to the clinic. To date, nearly all studies have attempted to characterize the components of protein coronas depending on different physiochemical properties of NPs. Herein, recent advances are reviewed in order to better understand the impact of the biological effects of the nanoparticle–corona on nanomedicine applications. The recent development of the impact of protein corona formation on the pharmacokinetics of nanomedicines is also highlighted. Finally, the challenges and opportunities of nanomedicine toward future clinical applications are discussed.

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“…This methodology is not optics based, and thus can also be used in turbid environment (in the presence of cells). [ 22 ] Nienhaus et al quantitatively analyzed the adsorption of human serum albumin (HSA) on small polymer‐coated NPs by using FCS. They precisely determined the essential kinetic, equilibrium, and structural parameters of protein binding to NPs.…”

Section: Main Influence Factors Of the Pc On The Surface Of Gnpsmentioning

confidence: 99%

Biological Behavior Regulation of Gold Nanoparticles via the Protein Corona

Hong

Ding

2020

Adv Healthcare Materials

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One of the difficulties in the translation of gold nanoparticles (GNPs) into clinical practice is the formation of the protein corona (PC) that causes the discrepancy between the in vitro and in vivo performance of GNPs. The PC formed on the surface of GNPs gives them a biological identity instead of an initial synthetic one. In most instances, this biological identity increases the particle size, leads to more clearance by the reticuloendothelial system, and causes less uptake by target cells. However, the performance of GNPs can still be improved by rewriting their original surface chemistry via the PC. This review specifically focuses on discussing the main influence factors, including the biological environment and physicochemical properties of GNPs, which affect the production and status of the PC. The status of the PC such as the amount, thickness, and composition subsequently influence the biological behavior of GNPs, especially their cellular uptake, cytotoxicity, biodistribution, and tumor targeting. Further understanding and revealing the impacts of the PC on the biological behavior of GNPs can be a promising and important strategy to regulate and improve the performance of GNP‐based biosystems in the future.

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In situ detection of the protein corona in complex environments

Cited by 123 publications

References 24 publications

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

The Crown and the Scepter: Roles of the Protein Corona in Nanomedicine

Biological Behavior Regulation of Gold Nanoparticles via the Protein Corona

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