In situ detection of nuclear atypia in Barrett's esophagus by using angle-resolved low-coherence interferometry

Pyhtila, John W.; Chalut, Kevin J.; Boyer, Jeffrey D.; Keener, Justin D.; D’Amico, Thomas A.; Gottfried, Marcia R.; Gress, Frank G.; Wax, Adam

doi:10.1016/j.gie.2006.10.016

Cited by 49 publications

(44 citation statements)

References 9 publications

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“…For example, Mie theory-based models typically require assumptions that determine the refractive index of the spheres and the surrounding medium as well as the size distribution of the spheres; in other words, some a priori knowledge of the phase function is required. Despite the lack of a robust tissue scattering model, optical spectroscopy of epithelial tissue has been demonstrated to have the potential for noninvasive cancer diagnostics, [9][10][11][12][13][14] an attractive alternative to the conventional biopsy and pathology approach. The promising results obtained in these studies underscore the importance of knowing the optical properties of the tissue under investigation.…”

Section: Introductionmentioning

confidence: 99%

Measurement of optical scattering properties with low-coherence enhanced backscattering spectroscopy

Turzhitsky¹,

Radosevich²,

Rogers³

et al. 2011

J. Biomed. Opt.

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Abstract. Low-coherence enhanced backscattering (LEBS) is a depth selective technique that allows noninvasive characterization of turbid media such as biological tissue. LEBS provides a spectral measurement of the tissue reflectance distribution as a function of distance between incident and reflected ray pairs through the use of partial spatial coherence broadband illumination. We present LEBS as a new depth-selective technique to measure optical properties of tissue in situ. Because LEBS enables measurements of reflectance due to initial scattering events, LEBS is sensitive to the shape of the phase function in addition to the reduced scattering coefficient (μ * s ). We introduce a simulation of LEBS that implements a two parameter phase function based on the Whittle-Matérn refractive index correlation function model. We show that the LEBS enhancement factor (E) primarily depends on μ * s , the normalized spectral dependence of E (S n ) depends on one of the two parameters of the phase function that also defines the functional type of the refractive index correlation function (m), and the LEBS peak width depends on both the anisotropy factor (g) and m. Three inverse models for calculating these optical properties are described and the calculations are validated with an experimental measurement from a tissue phantom. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Section: Introductionmentioning

confidence: 99%

Measurement of optical scattering properties with low-coherence enhanced backscattering spectroscopy

Turzhitsky¹,

Radosevich²,

Rogers³

et al. 2011

J. Biomed. Opt.

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“…[15][16][17][18] Recently, a/LCI has shown the ability to detect dysplasia in the epithelium of human esophagus. [19][20][21] This study evaluates the ability of a/LCI to detect dysplasia in situ in epithelial tissue samples from a human colon following surgical resection.…”

Section: Introductionmentioning

confidence: 99%

Detection of intestinal dysplasia using angle-resolved low coherence interferometry

et al. 2011

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Abstract. Angle-resolved low coherence interferometry (a/LCI) is an optical biopsy technique that allows for depth-resolved, label-free measurement of the average size and optical density of cell nuclei in epithelial tissue to assess the tissue health. a/LCI has previously been used clinically to identify the presence of dysplasia in Barrett's Esophagus patients undergoing routine surveillance. We present the results of a pilot, ex vivo study of tissues from 27 patients undergoing partial colonic resection surgery, conducted to evaluate the ability of a/LCI to identify dysplasia. Performance was determined by comparing the nuclear morphology measurements with pathological assessment of co-located physical biopsies. A statistically significant correlation between increased average nuclear size, reduced nuclear density, and the presence of dysplasia was noted at the basal layer of the epithelium, at a depth of 200 to 300 μm beneath the tissue surface. Using a decision line determined from a receiver operating characteristic, a/LCI was able to separate dysplastic from healthy tissues with a sensitivity of 92.9% (13/14), a specificity of 83.6% (56/67), and an overall accuracy of 85.2% (69/81). The study illustrates the extension of the a/LCI technique to the detection of intestinal dysplasia, and demonstrates the need for future in vivo studies. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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“…We now describe three a/LCI studies of BE tissues. The first two examined ex vivo resected esophageal tissues, in laboratory and clinical settings [29,35]. The third study was a pilot clinical trial in patients undergoing routine surveillance for BE which compared a/LCI measurements to co-registered traditional biopsies to assess the accuracy of the approach in detecting dysplasia [36].…”

Section: Clinical Application To Dysplasia In Barrett's Esophagusmentioning

confidence: 99%

“…[35]. Tissue samples from three patients with dysplastic BE who underwent esophago-gastrectomies were examined in the laboratory within 2 hours of surgery.…”

Section: Ex Vivo Studiesmentioning

confidence: 99%

Nuclear Morphology Measurements with Angle-Resolved Low Coherence Interferometry For Application To Cell Biology And Early Cancer Detection

Wax

Chalut

2011

Analytical Cellular Pathology

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Abstract. The study of intact, living cells using non-invasive optical spectroscopic methods offers the opportunity to assess cellular structure and organization in a way that is not possible with commonly used cell biology imaging techniques. We have developed a novel spectroscopic technique for diagnosing disease at the cellular level based on using low-coherence interferometry (LCI) to detect the angular distribution of scattered light. Angle-resolved LCI (a/LCI) combines the ability of LCI to isolate scattering from sub-surface tissue layers with the ability of light scattering spectroscopy to obtain structural information on sub-wavelength scales. In application to examining cellular structure, a/LCI enables quantitative measurements of changes in the size and texture of cell nuclei. These quantitative measurements are characteristic of different pathological states. The capabilities of a/LCI were demonstrated using a clinical system that can be applied in endoscopic surveillance of esophageal tissue, producing high sensitivity and specificity for detecting dysplastic tissues in vivo. Experiments with in vitro cell samples also show the utility of a/LCI in observing structural changes due to environmental stimuli as well as detecting apoptosis due to chemotherapeutic agents.

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In situ detection of nuclear atypia in Barrett's esophagus by using angle-resolved low-coherence interferometry

Cited by 49 publications

References 9 publications

Measurement of optical scattering properties with low-coherence enhanced backscattering spectroscopy

Measurement of optical scattering properties with low-coherence enhanced backscattering spectroscopy

Detection of intestinal dysplasia using angle-resolved low coherence interferometry

Nuclear Morphology Measurements with Angle-Resolved Low Coherence Interferometry For Application To Cell Biology And Early Cancer Detection

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