In situ detection of exosomal RNAs for cancer diagnosis

Sun, Zhiwei; Wu, Yanqiu; Gao, Fucheng; Li, Hui; Wang, Chuanxin; Li, Du; Li, Dong; Jiang, Yanyan

doi:10.1016/j.actbio.2022.10.061

Cited by 10 publications

(4 citation statements)

References 124 publications

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“…Exosome detection by binding to fluorescent nanoparticles labeled aptamers/antibodies has the advantage of high specificity. [ 102 ] Wu et al. constructed a dual‐encoding system combined with an in situ rolling circle amplification (RCA) signal amplification strategy to realize exosome recognition ( Figure A).…”

Section: Overview Of Exosome Analysis Based On Four Types Of Microflu...mentioning

confidence: 99%

“…[101] Exosome detection by binding to fluorescent nanoparticles labeled aptamers/antibodies has the advantage of high specificity. [102] Wu et al constructed a dual-encoding system combined with an in situ rolling circle amplification (RCA) signal amplification strategy to realize exosome recognition (Figure 6A). [103] In this work, a color-and size-based double-coded microsphere library was constructed by introducing quantum dots of different colors into microspheres of different sizes in proportion (Figure 6B).…”

Section: Channel Microfluidicmentioning

confidence: 99%

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Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

Sun

Gao

et al. 2023

Adv Materials Technologies

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Exosomes are a type of small extracellular vesicles carrying proteins, nucleic acids, and lipids. Because their expression levels are highly correlated with tumor development, exosomes are regarded as non‐invasive biomarkers for cancer diagnosis. Therefore, many exosome detection techniques are developed to provide a new way for cancer diagnosis. However, conventional techniques require the isolation of exosomes from body fluids before analysis, which usually relies on complex equipment and cumbersome operations. In addition, complex procedures may introduce impurities and thus affect the accuracy of the test. As an emerging analysis technology, microfluidic overcomes these shortcomings by integrating a microchannel for sample transport isolation and an exosome analysis module, showing great prospects in cancer diagnosis. This review will provide an overview of microchannel fabrication and analysis methods with outstanding compatibility for different microfluidic types, with a particular focus on microfluidic‐based exosome analysis methods that have the potential for clinical application.

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Section: Overview Of Exosome Analysis Based On Four Types Of Microflu...mentioning

confidence: 99%

Section: Channel Microfluidicmentioning

confidence: 99%

Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

Sun

Gao

et al. 2023

Adv Materials Technologies

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“…In situ detection of exosomal miRNA is a method of transporting probes into exosomes. 11 It not only avoids time-consuming processes, but also improves diagnostic reliability. The in situ detection strategy for exosomal miRNAs is mainly based on dye-labeled fluorescence methods.…”

Section: Introductionmentioning

confidence: 99%

A label-free activatable biosensor for in situ detection of exosomal microRNAs based on DNA-AgNCs and hairpin type nucleic acid probes

Li,

Xiang

et al. 2024

Anal. Methods

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“…Additionally, recent studies have highlighted the potential of engineered EVs in various disease contexts, such as cancer, cardiovascular disease, and neurodegenerative disorders [2][3][4] . Novel techniques for isolating and characterizing EVs continue to be developed, and ongoing research is aimed at improving our understanding of EV biology and exploiting EVs as biomedical tools [5][6][7] . The special issue of "Interfacial Engineering Strategies for EV in vitro and in vivo Studies" aims to provide insights into the engineering logic and design rules of EVs by proper convergence of EV intrinsic physiochemical properties and the "add-on" functional ligands.…”

Section: Introductionmentioning

confidence: 99%

New insights of engineered extracellular vesicles as promising therapeutic systems

Cheng¹,

Liu²,

Liao³

et al. 2023

Extracell Vesicles Circ Nucleic Acids

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Extracellular vesicles (EVs) are natural biological particles that carry and deliver molecular fingerprints from parental cells to receptor cells, where they take effect. EVs are widely recognized for their role as intercellular communication mediators and high correlation with disease evolution, making them a valuable target in many aspects, especially biomarker profiling and therapeutics. In the past decade, scientists from various disciplines, including biology, physics, chemistry, materials science, electrical engineering, and mechanical engineering, have jointly devoted efforts to advance the study of EVs from fundamental molecular mechanisms to EV-based translational medicine, covering EV marker-based diagnostics and EV-based drug delivery. Diverse interfacial engineering strategies have been developed to facilitate in vitro and in vivo studies of EVs. This special issue, titled "Interfacial Engineering Strategies for EV in vitro and in vivo Studies", focuses on understanding the engineering logic and design rules of EVs in biomedical fields, highlighting their therapeutic potential in combating many diseases. This will provide new insights into the construction of promising diagnostic and therapeutic systems.

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In situ detection of exosomal RNAs for cancer diagnosis

Cited by 10 publications

References 124 publications

Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

Microfluidic Chips Fabrication and Integration for Tumor‐Derived Exosomes Detection

A label-free activatable biosensor for in situ detection of exosomal microRNAs based on DNA-AgNCs and hairpin type nucleic acid probes

New insights of engineered extracellular vesicles as promising therapeutic systems

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