In situ detection, by spin trapping, of hydroxyl radical markers produced from ionizing radiation in the tumor of a living mouse.

Halpern, Howard J.; Cheng, Yu; Barth, Eugene D.; Perić, Miroslav; Rosen, Gerald M.

doi:10.1073/pnas.92.3.796

Cited by 80 publications

(48 citation statements)

References 35 publications

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“…As mentioned in Eq. 2&3, radiation on water in cell can also be regarded as another resource of hydroxyl radical [13]. Similarly, hydroxyl radicals, once generated, can also induce DNA oxidation to give rise to various oxidative lesions which is related to cancers.…”

Section: Resultsmentioning

confidence: 99%

Amentoflavone Protects against Hydroxyl Radical-induced DNA Damage via Antioxidant Mechanism

Li¹,

Wang²,

Han³

et al. 2014

Turk J Bioch

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Section: Resultsmentioning

confidence: 99%

Amentoflavone Protects against Hydroxyl Radical-induced DNA Damage via Antioxidant Mechanism

Li¹,

Wang²,

Han³

et al. 2014

Turk J Bioch

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“…The extension of spin trapping to enable direct measurements in vivo became feasible with the development of low frequency EPR (≤1,200 MHz) spectrometers with sufficient sensitivity (20,23,38). This ability to make the measurements immediately under physiological conditions has the potential to significantly increase our understanding of physiological and/or pathological roles of free radicals in biology and medicine.…”

Section: In Vivo Spin Trappingmentioning

confidence: 99%

“…Lipidderived radicals have been detected after acute and chronic administration of ethanol using spin trapping, and the radical concentration increased further after acute administration of ethanol to alcohol-fed rats (80). The free radicals generated from ethanol have been observed directly in vivo, using high doses of irradiation (23). To date, this seems to be the only successful study directly detecting free radicals generated from ethanol in living animals.…”

Section: Spin Trapping Of Carbon- Oxygen- and Sulfur-centered Radicalsmentioning

confidence: 99%

“…Recently, techniques such as high performance liquid chromatograph (HPLC), gas chromatography/mass spectroscopy (GC/ MS), liquid chromatography/mass spectroscopy (LC/MS) have been developed for chemical identification of all the radicals formed (120). While these methods require an ex vivo sample collection and analysis, they can be very useful complementary techniques for in vivo EPR spin trapping.The extension of spin trapping to enable direct measurements in vivo became feasible with the development of low frequency EPR (≤1,200 MHz) spectrometers with sufficient sensitivity (20,23,38). This ability to make the measurements immediately under physiological conditions has the potential to significantly increase our understanding of physiological and/or …”

mentioning

confidence: 99%

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Use of Electron Paramagnetic Resonance Spectroscopy to Evaluate the Redox StateIn Vivo

Swartz

Khan

Khramtsov

2007

Antioxidants & Redox Signaling

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The aim of this article is to provide an overview of how electron paramagnetic resonance (EPR) can be used to measure redox-related parameters in vivo. The values of this approach include that the measurements are made under fully physiological conditions, and some of the measurements cannot be made by other means. Three complementary approaches are used with in vivo EPR: the rate of reduction or reactions of nitroxides, spin trapping of free radicals, and measurements of thiols. All three approaches already have produced unique and useful information. The measurement of the rate of decrease of nitroxides technically is the simplest, but difficult to interpret because the measured parameter, reduction in the intensity of the nitroxide signal, can occur by several different mechanisms. In vivo spin trapping can provide direct evidence for the occurrence of specific free radicals in vivo and reflect relative changes, but accurate absolute quantification remains challenging. The measurement of thiols in vivo also appears likely to be useful, but its development as an in vivo technique is at an early stage. It seems likely that the use of in vivo EPR to measure redox processes will become an increasingly utilized and valuable tool. OVERVIEWThe goal of this article is to indicate how the unique capabilities of EPR can be used very effectively to follow redox status/events in vivo. This will be done in the context of a general overview and two more detailed complementary mini-reviews of the current status of the use of in vivo EPR to measure free radicals and thiols. The article also seeks to delineate areas where developments are needed and the potential pathways to achieve them.The ability to measure redox status and redox processes in vivo is very attractive for several reasons. It has been clearly established that redox-active species are intrinsically involved in many physiological and pathophysiological processes. The roles of these species include being essential intermediates in key physiological reactions and, in many cases, being directly involved in the causal chain leading to pathology. The redox state is an important parameter for many key events in cell signaling (13). Also, therapeutic approaches for many types of pathophysiology involve redox reactions (84).Whereas many useful insights can be achieved by measurements in model systems and cell cultures, the complexity of redox processes and physiology makes it difficult to understand fully the redox processes that occur in vivo without making direct measurements with the intact NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript system. The dynamics of the vascular system, metabolism in different organs, and the complex web of oxidants and antioxidants, cannot be modeled readily. There are few techniques that are capable of making such measurements in vivo, but fortunately EPR can follow many of the most important aspects of these complex processes.Some of the redox-active species are free radicals, and for these, EPR is t...

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“…However, with the development of low-frequency electron paramagnetic resonance (EPR) spectrometers capable of detecting paramagnetic species in living animals in real time (12)(13)(14)(15)(16), EPR imaging is now an alternative technology that can, like MRI, address significant physiological questions. The challenge, however, has been the synthesis of paramagnetic spin probes that can provide these essential data.…”

mentioning

confidence: 99%

Very‐low‐frequency electron paramagnetic resonance (EPR) imaging of nitroxide‐loaded cells

Kao

Barth

Burks

et al. 2007

Magnetic Resonance in Med

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In situ detection, by spin trapping, of hydroxyl radical markers produced from ionizing radiation in the tumor of a living mouse.

Cited by 80 publications

References 35 publications

Amentoflavone Protects against Hydroxyl Radical-induced DNA Damage via Antioxidant Mechanism

Amentoflavone Protects against Hydroxyl Radical-induced DNA Damage via Antioxidant Mechanism

Use of Electron Paramagnetic Resonance Spectroscopy to Evaluate the Redox StateIn Vivo

Very‐low‐frequency electron paramagnetic resonance (EPR) imaging of nitroxide‐loaded cells

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