In situ detection and characterization of biofilm in waters by electrochemical methods

Herbert-Guillou, D.; Tribollet, Bernard; Festy, D.; Kiéné, L.

doi:10.1016/s0013-4686(99)00310-2

Cited by 33 publications

(17 citation statements)

References 5 publications

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“…Modeling studies have predicted that the conductivity of the biofilm would have to be 10 À4 mS cm À1 to avoid electron transfer limitation (Marcus et al, 2007). Although biofilms are generally considered to act as insulators rather than conductors (Herbert-Guillou et al, 1999;Muñ ozBerbel et al, 2006;Dheilly et al, 2008), the finding that cells at substantial distance from the anode are metabolically active is consisent with the concept that anode biofilms may be conductive. Clearly, the more cells that can release electrons from metabolic activity, the greater the potential current output.…”

Section: Discussionmentioning

confidence: 99%

Microtoming coupled to microarray analysis to evaluate the spatial metabolic status of Geobacter sulfurreducens biofilms

et al. 2009

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Further insight into the metabolic status of cells within anode biofilms is essential for understanding the functioning of microbial fuel cells and developing strategies to optimize their power output. Cells throughout anode biofilms of Geobacter sulfurreducens reduced the metabolic stains: 5-cyano-2,3-ditolyl tetrazolium chloride and Redox Green, suggesting metabolic activity throughout the biofilm. To compare the metabolic status of cells growing close to the anode versus cells in the outer portion of the anode biofilm, anode biofilms were encased in resin and sectioned into inner (0-20 lm from anode surface) and outer (30-60 lm) fractions. Transcriptional analysis revealed that, at a twofold threshold, 146 genes had significant (Po0.05) differences in transcript abundance between the inner and outer biofilm sections. Only 1 gene, GSU0093, a hypothetical ATP-binding cassette transporter, had significantly higher transcript abundances in the outer biofilm. Genes with lower transcript abundance in the outer biofilm included genes for ribosomal proteins and NADH dehydrogenase, suggesting lower metabolic rates. However, differences in transcript abundance were relatively low (othreefold) and the expression of genes for the tricarboxylic acid cycle enzymes was not significantly lower. Lower expression of genes involved in stress responses in the outer biofilm may reflect the development of low pH near the surface of the anode. The results of this study suggest that cells throughout the biofilm are metabolically active and can potentially contribute to current production. The microtoming/microarray strategy described here may be useful for evaluating gene expression with depth in a diversity of microbial biofilms.

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Section: Discussionmentioning

confidence: 99%

Microtoming coupled to microarray analysis to evaluate the spatial metabolic status of Geobacter sulfurreducens biofilms

et al. 2009

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“…Biofilms of Escherichia coli and Pseudomonas aeruginosa grown on the two-electrode device described here were not conductive (17), and other microbial biofilms were also found to have poor conductivity (1,2,20). Other current-producing microorganisms such as Shewanella oneidensis (8) and Thermincola strain JR (31) do not form thick biofilms when producing current, suggesting that they are incapable of forming highly conductive biofilms.…”

mentioning

confidence: 83%

“…Most biofilms that have been studied are insulating (1,2,17,20). The possibility of electrically conductive biofilms was first suggested based on the findings that (i) Geobacter sulfurreducens produced thick (40 to 50 m) biofilms when growing on anode surfaces; (ii) biofilm cells not in contact with the anode contributed to current production as much as cells in direct contact; and (iii) the production of thick current-producing biofilms was dependent on the presence of conductive pili (25).…”

mentioning

confidence: 99%

Electrical Conductivity in a Mixed-Species Biofilm

Malvankar

Lau

Nevin

et al. 2012

Appl Environ Microbiol

101

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“…Modeling studies have also predicted that high current production by a thick anode associated biofilm is only feasible if the bacterial biofilm is conductive [118,119]. The production of a conductive biofilm is highly unusual as most biofilms act as insulators [120][121][122]. A major breakthrough in the field would be the measurement and determination of the conductivity, and the components responsible of conductivity, of a bacterial biofilm in a MFC.…”

Section: Electrical Interactions Between Microbes and Electrodesmentioning

confidence: 99%

Microbial Fuel Cells, A Current Review

2010

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Microbial fuel cells (MFCs) are devices that can use bacterial metabolism to produce an electrical current from a wide range organic substrates. Due to the promise of sustainable energy production from organic wastes, research has intensified in this field in the last few years. While holding great promise only a few marine sediment MFCs have been used practically, providing current for low power devices. To further improve MFC technology an understanding of the limitations and microbiology of these systems is required. Some researchers are uncovering that the greatest value of MFC technology may not be the production of electricity but the ability of electrode associated microbes to degrade wastes and toxic chemicals. We conclude that for further development of MFC applications, a greater focus on understanding the microbial processes in MFC systems is required.

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In situ detection and characterization of biofilm in waters by electrochemical methods

Cited by 33 publications

References 5 publications

Microtoming coupled to microarray analysis to evaluate the spatial metabolic status of Geobacter sulfurreducens biofilms

Microtoming coupled to microarray analysis to evaluate the spatial metabolic status of Geobacter sulfurreducens biofilms

Electrical Conductivity in a Mixed-Species Biofilm

Microbial Fuel Cells, A Current Review

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