In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration

Wu, Guangsheng; Feng, Chao; Quan, Jingjing; Wang, Zhongshan; Wei, Wei; Zang, Shengqi; Kang, Shuai; Guangyan, Hui; Chen, Xiguang; Wang, Qintao

doi:10.1016/j.carbpol.2017.10.090

Cited by 55 publications

(46 citation statements)

References 35 publications

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“…The usage of collagen as a carrier system for SDF-1 has also been shown for bone regeneration in dentistry as well as in in vivo applications. Further, other materials have been demonstrated to carry SDF-1 [52,53], which speaks for the good functionality of SDF-1 after release from carrier systems. These facts show its potential as a component to induce bone regeneration and for its use in TE with cell-free bone substitutes.…”

Section: Histological Analysesmentioning

confidence: 99%

Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

Lauer

Wolf

Mehler

et al. 2020

IJMS

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Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration.

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Section: Histological Analysesmentioning

confidence: 99%

Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

Lauer

Wolf

Mehler

et al. 2020

IJMS

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“…Therefore, the scaffolds usually demonstrate poor integration with surrounding bone tissues [ 12 ]. New materials and solutions are continually developed to meet medical needs [ 13 ]. Recently, the interaction between scaffolds and native tissues, particularly the indispensable role of the natural extracellular matrix (ECM) in bone repair, has been extensively investigated.…”

Section: Introductionmentioning

confidence: 99%

“…They are able to mimic the natural ECM of the bone, thus presenting a prospective ability to encapsulate bioactive molecules or cells. Due to the network structure of the hydrogels, the entrapped proteins or cells are confined in the meshes and they hydrogels can control the release of the materials as required [ 13 ]. Moreover, hydrogels are absorbable and demonstrate excellent integration with surrounding tissues, thereby avoiding the complexity of surgical removal and reducing the possibility of an inflammatory response [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Bioactive hydrogels for bone regeneration

Bai

Gao

Syed

et al. 2018

Bioactive Materials

386

248

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Bone self-healing is limited and generally requires external intervention to augment bone repair and regeneration. While traditional methods for repairing bone defects such as autografts, allografts, and xenografts have been widely used, they all have corresponding disadvantages, thus limiting their clinical use. Despite the development of a variety of biomaterials, including metal implants, calcium phosphate cements (CPC), hydroxyapatite, etc., the desired therapeutic effect is not fully achieved. Currently, polymeric scaffolds, particularly hydrogels, are of interest and their unique configurations and tunable physicochemical properties have been extensively studied. This review will focus on the applications of various cutting-edge bioactive hydrogels systems in bone regeneration, as well as their advantages and limitations. We will examine the composition and defects of the bone, discuss the current biomaterials for bone regeneration, and classify recently developed polymeric materials for hydrogel synthesis. We will also elaborate on the properties of desirable hydrogels as well as the fabrication techniques and different delivery strategies. Finally, the existing challenges, considerations, and the future prospective of hydrogels in bone regeneration will be outlined.

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“…Hydrogels are hydrated 3D polymeric networks that mimic the architecture of native tissue microenvironment and can modulate their structures to match the size and shape of defects. The network structure of hydrogels facilitates protein and cell entrapment and regulates release of these molecules . Porous structure of hydrogels also promotes nutrient and gaseous exchange.…”

Section: Introductionmentioning

confidence: 99%

Drug‐Loaded Elastin‐Like Polypeptide–Collagen Hydrogels with High Modulus for Bone Tissue Engineering

Pal

Nguyen

Benton

et al. 2019

Macromolecular Bioscience

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Emphasizing the role of hydrogel stiffness and cellular differentiation, this study develops collagen and elastin-like polypeptide (ELP)-based bone regenerative hydrogels loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2) and doxycycline with mechanical properties suitable for osteogenesis. The drug-incorporated collagen-ELP hydrogels has significantly higher modulus of 35 ± 5 kPa compared to collagen-only hydrogels. Doxycycline shows a bi-phasic release with an initial burst release followed by a gradual release, while rhBMP-2 exhibits a nearly linear release profile for all hydrogels. The released doxycycline shows anti-microbial activity against Pseudomonas aeruginosa, Streptococcus sanguinis, and Escherichia coli. Microscopic observation of the hydrogels reveals their interconnected, macroporous, 3D open architecture with pore diameters between 160 and 400 µm. This architecture supports human adipose-derived stem cell attachment and proliferation from initial days of cell seeding, forming a thick cellular sheath by day 21. Interestingly, in collagen and collagen-ELP hydrogels, cell morphology is elongated with stretched slender lamellipodial formation, while cells assemble as spheroidal aggregates in crosslinked as well as drugloaded hydrogels. Osteogenic markers, alkaline phosphatase and osteocalcin, are expressed maximally for drug-loaded hydrogels compared to those without drugs. The drug-loaded collagen-ELP hydrogels are thus promising for combating bacterial infection and promoting guided bone regeneration. 2 of 13) www.advancedsciencenews.com www.mbs-journal.de (

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In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration

Cited by 55 publications

References 35 publications

Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

Bioactive hydrogels for bone regeneration

Drug‐Loaded Elastin‐Like Polypeptide–Collagen Hydrogels with High Modulus for Bone Tissue Engineering

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