In Situ Control of Cell Adhesion Using Photoresponsive Culture Surface

Edahiro, Jun-ichi; Sumaru, Kimio; Tada, Yuichi; Ohi, Katsuhide; Takagi, Toshiyuki; Kameda, Mitsuyoshi; Shinbo, Toshio; Kanamori, Toshiyuki; Yoshimi, Yasuo

doi:10.1021/bm0493382

Cited by 200 publications

(126 citation statements)

References 18 publications

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“…This approach has been extended for patterning of surface with two different biotinylated proteins using pH sensitive photoresist [48]. Photochemical patterning can also be applied to detachment of cells using a combination of a spirobenzopyran with thermosensitive N-isopropylacrylamide by a combination of UV exposure and low-temperature washing [49].…”

Section: Open Accessmentioning

confidence: 99%

Photochemical Patterning of Ionically Cross-Linked Hydrogels

2013

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Iron(III) cross-linked alginate hydrogel incorporating sodium lactate undergoes photoinduced degradation, thus serving as a biocompatible positive photoresist suitable for photochemical patterning. Alternatively, surface etching of iron(III) cross-linked hydrogel contacting lactic acid solution can be used for controlling the thickness of the photochemical pattering. Due to biocompatibility, both of these approaches appear potentially useful for advanced manipulation with cell cultures including growing cells on the surface or entrapping them within the hydrogel.

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Section: Open Accessmentioning

confidence: 99%

Photochemical Patterning of Ionically Cross-Linked Hydrogels

2013

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“…Light responsive materials are typically triggered by two methods: 1) due to conformational changes of certain dye molecules found either in the polymer backbones or as pendent groups in the polymer chemical structure (Higuchi et al, 2004;Edahiro et al, 2005;Jiang et al, 2006;Nayak et al, 2006;Garcia et al, 2007;Zhu et al, 2007); 2) due to local heat generation of dyes linked to a thermally responsive polymer or hydrogel (Nayak and Lyon, 2004;Slocik et al, 2007). However, dyes are usually chemically reactive, susceptible to bleaching, and their responses are generally very slow (Kungwatchakun and Irie, 1988;Hugel et al, 2002;Shimoboji et al, 2002;Brinker, 2004).…”

Section: Constructing Non-mechanical Valves Using Locally-triggered Rmentioning

confidence: 99%

Environmentally responsive polymeric "intelligent" materials: the ideal components of non-mechanical valves that control flow in microfluidic systems

Morones‐Ramírez¹

2010

Braz. J. Chem. Eng.

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-Miniaturization and commercialization of integrated microfluidic systems has had great success with the development of a wide variety of techniques in microfabrication, since they allowed their construction at a low cost and by following simple step-series procedures. However, one of the major challenges in the design of microfluidic systems is to achieve control of flow and delivery of different chemical reagents. This feature is especially important when using microfluidic systems in the development of cell culture systems, the construction of labs on a chip and the fabrication and design of chemical microreactors. Spatiotemporal control of the microenvironment in microfluidic devices has been only partially achieved by incorporating actuator parts (mechanical and non-mechanical) within these devices; nevertheless, recently there has been enormous progress due to advances in the materials sciences, and the development of novel polymeric "intelligent" materials. These materials have proved to be excellent candidates in the construction of non-mechanical actuators in the form of environmentally responsive valves. These valves can more efficiently control flows because these "intelligent" materials are capable of undergoing conformational changes and phase transitions in response to different local or external environmental stimuli; allowing them to turn the valves from "on" to "off". In addition, these valves have very simple designs, and are easy and cheap to incorporate into microfluidic systems. Therefore, although there are many reviews that focus on the development and design of non-mechanical actuators, the following review proceeds to describe the exciting characteristics, potential uses and synthesis methods of the building blocks of the most recent and innovative non-mechanical valves, environmentally responsive polymeric "intelligent" materials. In addition, the last section of this review will focus on the synthesis of composite materials that are capable of responding to more than one type of stimulus, since these materials are believed to be the future components that will boost the development of microfluidic systems with spatiotemporal controlled microenvironments.

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“…65 Sumaru's group developed spiropyran-grafted PNIPAAm, which showed reversible switching of cell adhesiveness in response to both heat and light. 66 Compared to heat and voltage, light can be applied to substrates with higher spatial and temporal resolutions. Local regions of the substrates can easily be photoactivated by narrowing the irradiating regions.…”

Section: ·3 Substrates Responsive To Lightmentioning

confidence: 99%

Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences

et al. 2008

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Cell micropatterning is a method for controlling the placement of living cells on a substrate surface. [1][2][3][4][5][6][7][8] It is important for a wide range of applications, such as tissue engineering, cellbased drug screening, and fundamental cell biology studies. Most cell micropatterning methods fall into three categories based on strategy: (1) seeding cells on a chemically patterned surface of different cell adhesiveness, (2) seeding cells on a topographically patterned surface, or (3) directed delivery of cells onto discrete regions of a substrate. Although the latter two categories are also important, this review mainly focuses on the first category. This is because it is the most common strategy and it provides tools to study fundamental aspects of cell adhesion at the single protein/receptor molecule level. 9There are excellent reviews that also cover the latter two categories. 3,8,10 We first introduce some of the important applications of cell micropatterning in general. We then describe methods for micropatterning the cell adhesiveness, referring to materials that promote or inhibit cell adhesion. We then move on to much more sophisticated substrates, so-called "dynamic substrates", whose cell adhesiveness can be changed by an external stimulus, such as heat, voltage and light. As an example of dynamic substrates, we describe a caged culture substrate developed by our group. This type of functional substrate opens up new possibilities in bioanalytical and biomedical sciences. Cell micropatterning is an important technique for a wide range of applications, such as tissue engineering, cell-based drug screening, and fundamental cell biology studies. This paper overviews cell patterning techniques based on chemically modified substrates with different degrees of cell adhesiveness. In particular, the focus is on dynamic substrates that change their cell adhesiveness in response to external stimuli, such as heat, voltage, and light. Such substrates allow researchers to achieve an in situ alteration of patterns of cell adhesiveness, which is useful for coculturing multiple cell types and analyzing dynamic cellular activities. As an example of dynamic substrates, we introduce a dynamic substrate based on a caged compound, where we accomplished a light-driven alteration of cell adhesiveness and the analysis of a single cell's motility.

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In Situ Control of Cell Adhesion Using Photoresponsive Culture Surface

Cited by 200 publications

References 18 publications

Photochemical Patterning of Ionically Cross-Linked Hydrogels

Photochemical Patterning of Ionically Cross-Linked Hydrogels

Environmentally responsive polymeric "intelligent" materials: the ideal components of non-mechanical valves that control flow in microfluidic systems

Recent Advances in Cell Micropatterning Techniques for Bioanalytical and Biomedical Sciences

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