In situ characterisation of a microorganism surface by Raman microspectroscopy: the shell of Ascaris eggs

Quilès, Fabienne; Balandier, Jean‐Yves; Capizzi-Banas, Sandrine

doi:10.1007/s00216-006-0638-4

Cited by 42 publications

(33 citation statements)

References 18 publications

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“…Given favorable environmental conditions, they have been reported to survive for up to 15 years [3] . They are also very sticky [7] , and attach easily to fruit, vegetables, soil and dust particles, children's toys, currency notes, flies and cockroaches [3,[8][9][10] .…”

Section: Life History and Epidemiologymentioning

confidence: 99%

Ascaris lumbricoides: A Review of Its Epidemiology and Relationship to Other Infections

Scott¹

2008

Ann Nestlé [Engl]

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This review highlights advances made since 2004 in understanding the epidemiology of infection and the interactions between Ascarislumbricoides and other concurrent infections. As water scarcity increases, untreated wastewater is increasingly used to irrigate crops, thus increasing the risk of transmission. New methods to detect and inactivate Ascaris eggs in water, soil and food are described. The association between pig ownership and Ascaris infection in humans may represent cross-transmission as hybridization among the pig and human ascarids occurs more frequently than previously believed. Geospatial analyses have successfully predicted infection levels both at a regional level (based on vegetation indices, temperature and humidity) and within communities (based on social and environmental factors). The interpretation of antibody and cytokine responses to Ascaris is becoming clearer, as researchers recognize the role of antigen type, age, the history of Ascaris and other infections. The considerable interest emerging on the interactions between Ascaris and other infections (helminths, malaria, HIV, tuberculosis) and allergy is explored. The impact of concurrent infection on the design of control strategies is discussed including the benefits arising from combination therapies and the evidence that intestinal nematodes impair the efficacy of childhood vaccines. Finally, recommended areas for future research are identified.

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Section: Life History and Epidemiologymentioning

confidence: 99%

Ascaris lumbricoides: A Review of Its Epidemiology and Relationship to Other Infections

Scott¹

2008

Ann Nestlé [Engl]

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“…The possible saturation with helminth eggs during previous experiments could also have influenced the stability of the system with respect to helminth egg removal. Since none of the eggs are spherical [1,3,36,37], it is likely that they settle with different, but unknown orientations [64]. , are shown in Figures 8 and 9.…”

Section: Resultsmentioning

confidence: 99%

“…This helminth egg is very resistant to inactivation under different environmental conditions [1,4]. This resistance is related to their four-layered shell composed of a lipid layer with a total thickness of about 4.5 μm, a mechanically rigid chitinous layer, a vitelline membrane and an external coat [3,36,37]. The shell is sensitive to lipid solvents and shows reduced surfaces and ridges.…”

Section: Introductionmentioning

confidence: 99%

“…The shell is sensitive to lipid solvents and shows reduced surfaces and ridges. This mammillated layer is bile-stained to a golden brown color, and its high hydration makes it limp in the natural environment [4,37]. Microorganisms present in anaerobic sludge may play a role in degrading nematode eggs, though limited research results are available.…”

Section: Introductionmentioning

confidence: 99%

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Helminth Egg Removal Capacity of UASB Reactors under Subtropical Conditions

Yaya-Beas

Ayala-Limaylla

Kujawa-Roeleveld

et al. 2015

Water

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This research was conducted to study the anaerobic sludge filtration capacity regarding helminth egg removal in upflow anaerobic sludge blanket (UASB) reactors. Two 25 L lab-scale UASB reactors were operated at an ambient temperature which varied between 17.1 and 28.6 °C. Ascaris suum egg was selected as the model egg considering its similarity in terms of size and morphology to Ascaris lumbricoides, a human pathogen. Ascaris suum eggs were obtained from female parasites of infected pigs. The anaerobic sludge filtration capacity was performed applying upflow velocities between 0.09 and 0.68 m·h −1. Three sludge bed heights in the range of 0.30-0.40 m, 0.50-0.60 m and 0.60-0.70 m were applied. These sludge bed heights corresponded to 19%-25%, 31%-38% and 38%-44% of the total reactor height, respectively. Under the mentioned conditions, the average helminth egg removal efficiency was reciprocally correlated to the imposed upflow velocity. The studied lab-scale reactors reported an average helminth egg removal between 34%-100%, 30%-91% and 34%-56%, when the sludge bed in the UASB reactor OPEN ACCESSWater 2015, 7 2403 was 19%-25%, 31%-38% and 38%-44% of the total reactor height, respectively. The decreased filtration capacity at increasing sludge bed heights might be likely related to biogas production and channeling formation. The average helminth egg removal efficiency in the control experiments performed without any sludge bed, by plain sedimentation, varied between 44% and 66%.

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“…Si las condiciones ambientales son favorables se ha descrito que sobreviven durante periodos de hasta 15 años [3] . Son también muy adherentes [7] y se acoplan fácilmente a frutas, verduras, partículas de tierra y polvo, juguetes de niños, billetes de banco, moscas y cucarachas [3,[8][9][10] .…”

Section: Historia De Vida Y Epidemiologíaunclassified

Ascaris lumbricoides: Una revisión de su epidemiología y su relación con otras infecciones

Scott¹

2008

Ann Nestlé [Esp]

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En esta revisión se realzan los progresos efectuados desde 2004 en el conocimiento de la epidemiología de la infección y las interacciones entre Ascaris lumbricoides y otras infecciones concomitantes. A medida que aumenta la escasez de agua se utilizan cada vez más aguas residuales no tratadas para regar cultivos, aumentando de este modo el riesgo de transmisión. Se describen métodos nuevos para detectar e inactivar los huevos de Ascaris en el agua, el suelo y los alimentos. La asociación entre la posesión de cerdos y la infección por Ascaris en el humano puede indicar una transmisión cruzada, dado que la hibridización entre las ascárides porcinos y las ascárides humanos se produce más frecuentemente de lo que antes se creía. Los análisis geoespaciales han pronosticado con éxito los niveles de infección, tanto a nivel regional (basándose en índices de vegetación y en temperatura y humedad) como a nivel intracomunitario (basándose en factores sociales y ambientales). La interpretación de las respuestas de anticuerpos y citocinas a Ascaris está empezando a aclararse a medida que los investigadores identifican el papel del tipo de antígeno, la edad, la historia de Ascaris y otras infecciones. Se explora el considerable interés despertado por las interacciones entre Ascaris y otras infecciones (helmintos, paludismo, VIH, tuberculosis) y la alergia. Se discute el impacto de la infección concomitante sobre el diseño de las estrategias de control, incluyendo los beneficios derivados de tratamientos de combinación y las pruebas a favor de que los nemátodos intestinales deterioran la eficacia de las vacunas en la infancia. Por último se identifican ámbitos recomendados para la investigación futura.

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In situ characterisation of a microorganism surface by Raman microspectroscopy: the shell of Ascaris eggs

Cited by 42 publications

References 18 publications

<i>Ascaris lumbricoides</i>: A Review of Its Epidemiology and Relationship to Other Infections

<i>Ascaris lumbricoides</i>: A Review of Its Epidemiology and Relationship to Other Infections

Helminth Egg Removal Capacity of UASB Reactors under Subtropical Conditions

<i>Ascaris lumbricoides:</i> Una revisión de su epidemiología y su relación con otras infecciones

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