In-situ carboxylic acid separation from mixed-acid fermentation of cellulosic substrates in batch culture

Roy, Samarpita; Olokede, Opeyemi; Wu, Haoran; Holtzapple, Mark T.

doi:10.1016/j.biombioe.2021.106165

Cited by 11 publications

(15 citation statements)

References 28 publications

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“…This rapid digestion can be explained by the low lignin content, the lack of inhibition because of the low product concentration in the digester, and the presence of soluble sugars in the biomass, as previously mentioned. 44 In contrast, many lignocellulosic biomass sources previously used in the carboxylate platform have a high lignin content that must be remediated through expensive pretreatment. Furthermore, lignocellulose contains sugar polymers (e.g., cellulose, hemicellulose) that must be hydrolyzed before acid production (acetogenesis and acidogenesis) can begin.…”

Section: Discussionmentioning

confidence: 99%

“…an Aceq yield of 0.54 g acetate equivalent/g NAVS fed . 44 Moreover, prickly pear attained greater yields without co-treatment or pretreatment.…”

Section: Maad Performance Parametersmentioning

confidence: 98%

“…and dried chicken manure to achieve 0.65 g NAVS digested /g NAVS fed . 44 However, prickly pear attained higher conversion without any in-situ product removal or chemical pretreatment.…”

Section: Maad Performance Parametersmentioning

confidence: 99%

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Valorizing prickly pear cladodes via methane‐arrested anaerobic digestion for carboxylic acid production

Olokede

Holtzapple

2022

Biotechnology Progress

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To address climate change, liquid biofuels are an essential alternative to fossil fuels, especially for transportation. The carboxylate platform uses methane‐arrested anaerobic digestion (MAAD) to ferment biomass to carboxylic acids, which can be chemically converted to liquid fuels via the carboxylate platform. Most biomass sources require expensive pretreatments to remove lignin; however, prickly pear (Opuntia ficus‐indica) cladodes have low lignin content and therefore do not require pretreatment. Furthermore, this sugar‐rich feedstock is readily digested to high concentrations of carboxylic acids. At various substrate concentrations, batch MAAD of prickly pear cladodes yielded primarily acetic, butyric, and caproic acids. From these batch data, continuum particle distribution modeling (CPDM) simulated four‐stage countercurrent digestion. At a non‐acid volatile solid (NAVS) concentration of 100 g/Lliq, CPDM predicts a high total acid concentration of 93 g/L and conversion of 0.93 g NAVSdigested/NAVSfed at a volatile solid loading rate of 6 g/(Lliq·d) and liquid retention time of 35 days. Without chemical pretreatment, co‐digestion, or in situ product removal, prickly pear produced high yields, biomass conversion, product concentration, and selectivity compared to previously studied lignocellulosic feedstocks.

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Section: Discussionmentioning

confidence: 99%

“…an Aceq yield of 0.54 g acetate equivalent/g NAVS fed . 44 Moreover, prickly pear attained greater yields without co-treatment or pretreatment.…”

Section: Maad Performance Parametersmentioning

confidence: 98%

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Valorizing prickly pear cladodes via methane‐arrested anaerobic digestion for carboxylic acid production

Olokede

Holtzapple

2022

Biotechnology Progress

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“…Series chromatograph with a thermal conductivity detector (TCD) and (30 mm Â 0.320 mm). 37 Moisture and ash contents were determined as previously described. 20 Moisture content (MC) is defined as the amount of moisture vaporized from wet samples after baking at 105 C for 24 h.…”

Section: Methodsmentioning

confidence: 99%

“…Supernatant samples were analyzed using an Agilent 6890 Series chromatograph with an automatic liquid sampler (Agilent 7683 series), a flame ionization detector (FID), and a DB‐FFAP capillary column (30 mm × 0.320 mm) 37 . Moisture and ash contents were determined as previously described 20 .…”

Section: Methodsmentioning

confidence: 99%

Assessment of corn stover pretreated with shock and alkali using methane‐arrested anaerobic digestion

Olokede

Hsu

Huang

et al. 2022

Biotechnology Progress

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Corn stover, an underutilized agricultural residue, is a promising lignocellulosic feedstock for producing biofuels. To fully utilize it, pretreatment is needed. Typically, pretreatments are rapidly assessed using extracellular enzymes that release sugars from cellulose and hemicellulose. In contrast, this study uses methane‐arrested anaerobic digestion (MAAD) to assess pretreatments. Although time consuming, MAAD is a more accurate assessment technique when lignocellulose is employed in the carboxylate platform, a promising approach that utilizes nearly all biomass components. Using recommended pretreatment conditions identified from a previous study, three corn stover pretreatments were compared using MAAD: (1) shock‐only, (2) NaOH‐only, and (3) shock + NaOH. Air‐dried sewage sludge was used as nutrient source. At 100 g/L initial substrate concentration, compared to untreated corn stover, shock‐only decreased conversion (amount of biomass digested) by 14%, NaOH‐only increased conversion by 82%, and shock + NaOH increased conversion by 104%. Using batch MAAD data, the continuum particle distribution model simulated four‐stage countercurrent fermentation. At an industrial non‐acid volatile solids (NAVS) concentration of 300 g/Lliq, for both NaOH‐only and shock + NaOH, the model predicts total carboxylic acid concentration of about 58 g/L and conversion of about 0.85 g NAVSdigested/g NAVSfed at liquid retention time of 35 days and volatile solid loading rate of 4 g/(Lliq⋅day). At this long solid residence time, shock is not necessary; however, with short solid residence times, shock acts synergistically to aid NaOH pretreatment. Shock treatment offers a way to reduce pretreatment costs without sacrificing pretreatment efficacy.

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The Impact of Preservation Techniques on Methane-Arrested Anaerobic Digestion of Nutrient-Rich Feedstocks

Olokede

Liu

Holtzapple

2022

Appl Biochem Biotechnol

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In-situ carboxylic acid separation from mixed-acid fermentation of cellulosic substrates in batch culture

Cited by 11 publications

References 28 publications

Valorizing prickly pear cladodes via methane‐arrested anaerobic digestion for carboxylic acid production

Valorizing prickly pear cladodes via methane‐arrested anaerobic digestion for carboxylic acid production

Assessment of corn stover pretreated with shock and alkali using methane‐arrested anaerobic digestion

The Impact of Preservation Techniques on Methane-Arrested Anaerobic Digestion of Nutrient-Rich Feedstocks

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