Abstract:Sclerostin failed to restore the expression of the anabolic genes, we investigated the effect of Sclerostin on the activation of Wnt non canonical pathways mediated by Ca2+/CaMKII, JNK and PKC proteins. Methods: Primary murine chondrocytes were cultured with or without Wnt3a conditioned media and in the presence or absence of recombinant Sclerostin. Activation of Ca2+/CaMKII, JNK and PKC pathways was analyzed by Western blotting. The role of the activation of JNK and PKC pathways in the chondrocyte phenotype w… Show more
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