In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell–Derived Factor)-1α Peptide Eluting Vascular Grafts

Shafiq, Muhammad; Zhang, Qiuying; Zhi, Dengke; Wang, Kai; Kim, Dong Hwee; Kim, Soo Hyun

doi:10.1161/atvbaha.118.310934

Cited by 36 publications

(29 citation statements)

References 65 publications

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“…Various strategies have been employed to capture rare circulating endothelial progenitor cells, EPCs, from the blood to induce endothelialization of implanted materials 28,31,[38][39][40] . Some groups employed antibodies against EPC specific proteins such as VEGFR2, CD34, and CD133, but application in small animal models was met with varying success 40,41 .…”

Section: Discussionmentioning

confidence: 99%

Endothelialization of arterial vascular grafts by circulating monocytes

et al. 2020

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Recently our group demonstrated that acellular tissue engineered vessels (A-TEVs) comprised of small intestinal submucosa (SIS) immobilized with heparin and vascular endothelial growth factor (VEGF) could be implanted into the arterial system of a pre-clinical ovine animal model, where they endothelialized within one month and remained patent. Here we report that immobilized VEGF captures blood circulating monocytes (MC) with high specificity under a range of shear stresses. Adherent MC differentiate into a mixed endothelial (EC) and macrophage (Mφ) phenotype and further develop into mature EC that align in the direction of flow and produce nitric oxide under high shear stress. In-vivo, newly recruited cells on the vascular lumen express MC markers and at later times they co-express MC and EC-specific proteins and maintain graft patency. This novel finding indicates that the highly prevalent circulating MC contribute directly to the endothelialization of acellular vascular grafts under the right chemical and biomechanical cues.

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Section: Discussionmentioning

confidence: 99%

Endothelialization of arterial vascular grafts by circulating monocytes

et al. 2020

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“…The generation of ROS and mitochondrial dysfunction under the diabetic environment were weakened by SP and it caused the epithelial wound healing (36). In the case of blood vessel regeneration, SP induced the recruitment of stem cells and the regeneration of smooth muscle cells, collagen layer and blood vessels in vascular graft explanted models (37). The angiogenesis and re-epithelialization in wound area was enhanced by SP with increase of ECM deposition (38).…”

Section: Discussionmentioning

confidence: 99%

Substance P Improves Renal Ischemia Reperfusion Injury Through Modulating Immune Response

et al. 2020

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Substance P (SP), an injury-inducible messenger that mobilizes bone marrow stem cells and modulates the immune response, has been suggested as a novel target for therapeutic agents. We evaluated the role of SP as an immune cell modulator during the progression of renal ischemic/reperfusion injury (IRI). Unilateral IRI induced the transient expression of endogenous SP and the infiltration of CCR7 + M1 macrophages in injured kidneys. However, SP altered the intrarenal macrophage polarization from CCR7 + M1 macrophages to CD206 + M2 macrophages in injured kidneys. SP also modulated bone marrow-derived neutrophils and mesenchymal stromal cells after IRI. SP treatment for 4 weeks starting one week after unilateral IRI significantly preserved kidney size and length and normal tubular structures and alleviated necrotic tubules, inflammation, apoptosis, and tubulointerstitial fibrosis. The beneficial effects of SP were accompanied by attenuation of intrarenal recruitment of CD4, CD8, and CD20 cells and abnormal angiogenesis. The immunomodulatory effect of SP suggested that SP could be a promising therapeutic target for preventing the progression of acute kidney injury to chronic kidney disease.

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“…We used co-electrospinning from two separate spinnerets to fabricate cardiac patches. Electrospinning can afford a three-dimensional architecture, which may recapitulate the native tissue’s ECM and provide physical signals for tissue repair [ 29 , 30 ]. Herein, we revealed that the use of patches containing SP and IGF-1C peptide promote heart regeneration in vivo .…”

Section: Introductionmentioning

confidence: 99%

In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches

Shafiq

Zhang

Zhu

et al. 2018

Regenerative Biomaterials

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Cardiovascular diseases cause huge socio-economic burden worldwide. Although a mammalian myocardium has its own limited healing capability, scaffold materials capable of releasing stem cell recruiting/engrafting factors may facilitate the regeneration of the infarcted myocardium. The aim of this research was to develop cardiac patches capable of simultaneously eluting substance P (SP) and insulin-like growth factor-1C (IGF-1C) peptide. Polycaprolactone/collagen type 1-based patches with or without SP and IGF-1C peptide were fabricated by co-electrospinning, which exhibited nanofibrous morphology. SP and IGF-1C/SP patches recruited significantly higher numbers of bone marrow-mesenchymal stem cells than that of the negative control and patch-only groups in vitro. The developed patches were transplanted in an infarcted myocardium for up to 14 days. Mice underwent left anterior descending artery ligation and received one of the following treatments: (i) sham, (ii) saline, (iii) patch-only, (iv) IGF-1C patch, (v) SP patch and (vi) IGF-1C/SP patch. SP and IGF-1C/SP patch-treated groups exhibited better heart function and attenuated adverse cardiac remodeling than that of the saline, patch-only and individual peptide containing cardiac patches. SP patch and IGF-1C/SP patch-treated groups also showed higher numbers of CD31-positive vessels and isolectin B4-positive capillaries than that of other groups. IGF-1C/SP-treated group also showed thicker left ventricular wall in comparison to the saline and patch-only groups. Moreover, IGF-1C/SP patches recruited significantly higher numbers of CD29-positive cells and showed less numbers of Tunel-positive cells compared with the other groups. These data suggest that SP and IGF-1C peptides may act synergistically for in situ tissue repair.

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In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell–Derived Factor)-1α Peptide Eluting Vascular Grafts

Abstract: Supplemental Digital Content is available in the text.

Cited by 36 publications

References 65 publications

Endothelialization of arterial vascular grafts by circulating monocytes

Endothelialization of arterial vascular grafts by circulating monocytes

Substance P Improves Renal Ischemia Reperfusion Injury Through Modulating Immune Response

In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches

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