In Situ Assessment of the Brain Microcirculation in Mechanically-Ventilated Rabbits Using Sidestream Dark-Field (SDF) Imaging

Šitina, Michal; Turek, Z.; Parizkova, R; Černý, Viktor

doi:10.33549/physiolres.931937

Cited by 9 publications

(6 citation statements)

References 26 publications

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“…LDF appears to be the most reliable method for monitoring the microcirculation in the large bowel or rectum. Other methods include sidestream dark field imaging, which is based on orthogonal polarization spectral imaging, and intestinal microdialysis [5][6][7][8][9]. Larger studies, as well as comparisons of individual methods in larger samples, have yet to be conducted.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Cumulative Fluid Balance Impact on the Stability of Gastrointestinal Tract Anastomosis

et al. 2021

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Anastomotic leakage is a serious postoperative complication following a low anterior resection of rectum. Current research in colorectal surgery focuses on searching for techniques which could minimize the risk of leakage. The main objective of this study was to evaluate the impact of cumulative fluid balance on microcirculatory changes at the anastomotic site.This experimental study used 15 domestic pigs; all of the animals underwent rectal resection. Then the animals were divided into three groups and received IV crystalloids at various rates, i.e. 5 ml/kg/h; 10 ml/kg/h; and 20 ml/kg/h. Large bowel micro-perfusion was measured using laser Doppler flowmetry during and following the surgical procedure.The experiments were successfully performed in all 15 cases. No animal died during the procedure or during the follow-up. We found no differences in micro-perfusion of the bowel between the experimental groups during the surgical procedure and during the follow-up period after the surgery. A significant decrease in micro-perfusion was observed after the transection of the inferior mesenteric artery in all the groups; again, no differences between the groups were observed.We did not confirm the original hypothesis that excessive load of IV crystalloids during the surgery would have a negative effect on bowel micro-perfusion and thus a negative effect on the healing of the anastomosis. However, laser Doppler flowmetry was found to have high accuracy in measuring tissue microcirculation and has the potential to be used in clinical practice.

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Section: Discussionmentioning

confidence: 99%

Analysis of Cumulative Fluid Balance Impact on the Stability of Gastrointestinal Tract Anastomosis

et al. 2021

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“…A power analysis using an α error of 0.05 and β error of 0.2 was performed based on previously published brain microcirculatory data for rabbits [ 23 ] using MedCalc 7.6.0. (MedCalc Software, Ostend, Belgium).…”

Section: Methodsmentioning

confidence: 99%

“…SDF technology has been used to study changes in the microcirculation under various clinical conditions in both animal [ 21 ] and human studies [ 22 ]. SDF has also been used to investigate cortical brain microcirculation in animals with different pathological conditions, mainly in models of sepsis [ 23 – 26 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of hypertonic saline and mannitol on cortical cerebral microcirculation in a rabbit craniotomy model

et al. 2015

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BackgroundHyperosmolar solutions have been used in neurosurgery to modify brain bulk and prevent neurological deterioration. The aim of this animal study was to compare the short-term effects of equivolemic, equiosmolar solutions of mannitol and hypertonic saline (HTS) on cerebral cortical microcirculation in a rabbit craniotomy model.MethodsRabbits (weight, 2.0–3.0 kg) were anesthetized, ventilated mechanically, and subjected to a craniotomy. The animals were allocated randomly to receive a 3.75 ml/kg intravenous infusion of either 3.2 % HTS (group HTS, n = 8) or 20 % mannitol (group MTL, n = 8). Microcirculation in the cerebral cortex was evaluated using sidestream dark-field (SDF) imaging before and 20 min after the end of the 15-min HTS infusion. Global hemodynamic data were recorded, and blood samples for laboratory analysis were obtained at the time of SDF image recording.ResultsNo differences in the microcirculatory parameters were observed between the groups before the use of osmotherapy. After osmotherapy, lower proportions of perfused small vessel density (P = 0.0474), perfused vessel density (P = 0.0457), and microvascular flow index (P = 0.0207) were observed in the MTL group compared with those in the HTS group.ConclusionsOur findings suggest that an equivolemic, equiosmolar HTS solution better preserves perfusion of cortical brain microcirculation compared to MTL in a rabbit craniotomy model.

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“…sublingual area, brain, gut mucosa, gingiva, skin) and animals (e.g. liver, brain, intestinal mucosa, kidney, iris; Pennings et al ., ; Boerma et al ., ; Asfar et al ., ; Fischer et al ., ; Wauters et al ., ; Sitina et al ., ; Cerny et al ., ). The purpose of our study was to examine the reliability and reproducibility of RMIC assessment in rats by using SDF imaging technology.…”

Section: Introductionmentioning

confidence: 99%

In situ assessment of the renal microcirculation in mechanically ventilated rats using sidestream dark‐field imaging

Astapenko

Jor

Lehmann

et al. 2014

Journal of Microscopy

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For microcirculation research there is a need for baseline data and feasibility protocols describing microcirculation of various organs. The aim of our study was to examine the reliability and reproducibility of sidestream dark-field (SDF) imaging within the renal cortical microcirculation in rats. Renal microcirculation was observed using SDF probe placed on the exposed renal surface via the upper midline laparotomy. Video sequences recorded intermittently in short apneic pauses were analyzed off-line by using AVA 3.0 software (MicroVision Medical, Amsterdam, the Netherlands). Results are expressed as mean (SD) or median (25-75% percentiles). We obtained 60 clear sequences from all recorded analyzable videos from all the animals. The total small vessel and all vessel density (in mm.mm(-2) ) were (28.79 ± 0.40) and (28.95 ± 0.40), respectively. The perfused small and all vessel density were (28.79 ± 0.40) and (28.95 ± 0.40), respectively. The DeBacker Score was (19.14 ± 0.43), the proportion of perfused vessels was 100% (100-100%) and the microvascular flow index was 3.49 (3-3.75). We conclude SDF imaging provides a reliable method to examine the renal microvascular bed in vivo and thus can be used for the study of the renal cortical vascular network in various experimental diseases models and clinical settings.

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In Situ Assessment of the Brain Microcirculation in Mechanically-Ventilated Rabbits Using Sidestream Dark-Field (SDF) Imaging

Cited by 9 publications

References 26 publications

Analysis of Cumulative Fluid Balance Impact on the Stability of Gastrointestinal Tract Anastomosis

Analysis of Cumulative Fluid Balance Impact on the Stability of Gastrointestinal Tract Anastomosis

Effects of hypertonic saline and mannitol on cortical cerebral microcirculation in a rabbit craniotomy model

In situ assessment of the renal microcirculation in mechanically ventilated rats using sidestream dark‐field imaging

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