In situ assessment of mitochondrial calcium transport in tobacco pollen tubes

Flores-Herrera, Cesar; Preciado-Linares, Gisela; Gonzalez-Vizueth, Israel; Peña, Norma Corona de la; Gutiérrez‐Aguilar, Manuel

doi:10.1007/s00709-018-1316-z

Cited by 7 publications

(7 citation statements)

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“…KEGG enrichment analysis showed that the up-regulated proteins were significantly enriched in Oxidative phosphorylation signaling pathways. Oxidative phosphorylation plays an important role in maintaining pollen tube development and pollen fertility, and is the basis of regulating pollen formation and development (Flores-Herrera et al, 2019;Wei et al, 2020). In addition, MBF1 and cysteine proteinase inhibitor were most significantly up-regulated during pollen maturation.…”

Section: Discussionmentioning

confidence: 99%

Multi-omics research on common allergens during the ripening of pollen and poplar flocs of Populus deltoides

Wei

Luo

et al. 2023

Front. Plant Sci.

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BackgroundPopulus deltoides is widely cultivated in China and produces a large number of pollen and poplar flocs from March to June per year. Previous studies have found that the pollen of P. deltoides contains allergens. However, studies on the ripening mechanism of pollen/poplar flocs and their common allergens are very limited.MethodsProteomics and metabolomics were used to study the changes of proteins and metabolites in pollen and poplar flocs of P. deltoides at different developmental stages. Allergenonline database was used to identify common allergens in pollen and poplar flocs at different developmental stages. Western blot (WB) was used to detect the biological activity of common allergens between mature pollen and poplar flocs.ResultsIn total, 1400 differently expressed proteins (DEPs) and 459 different metabolites (DMs) were identified from pollen and poplar flocs at different developmental stages. KEGG enrichment analysis showed that DEPs in pollen and poplar flocs were significantly enriched in ribosome and oxidative phosphorylation signaling pathways. The DMs in pollen are mainly involved in aminoacyl-tRNA biosynthesis and arginine biosynthesis, while the DMs in poplar flocs are mainly involved in glyoxylate and dicarboxylate metabolism. Additionally, 72 common allergens were identified in pollen and poplar flocs at different developmental stages. WB showed that there were distinct binding bands between 70 and 17KD at the two groups of allergens.ConclusionsA multitude of proteins and metabolites are closely related to the ripening of pollen and poplar flocs of Populus deltoides, and they contain common allergens between mature pollen and poplar flocs.

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Section: Discussionmentioning

confidence: 99%

Multi-omics research on common allergens during the ripening of pollen and poplar flocs of Populus deltoides

Wei

Luo

et al. 2023

Front. Plant Sci.

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“…Protoplast permeability was determined under the microscope after staining for 5 minutes with 1% Evans blue followed by extensive washing with PAB. 35 …”

Section: Methodsmentioning

confidence: 99%

An Arabidopsis mutant line lacking the mitochondrial calcium transport regulator MICU shows an altered metabolite profile

Gutiérrez-Mireles,

Páez-Franco,

Rodríguez-Ruíz

et al. 2023

Plant Signaling & Behavior

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Plant metabolism is constantly changing and requires input signals for efficient regulation. The mitochondrial calcium uniporter (MCU) couples organellar and cytoplasmic calcium oscillations leading to oxidative metabolism regulation in a vast array of species. In Arabidopsis thaliana , genetic deletion of AtMICU leads to altered mitochondrial calcium handling and ultrastructure. Here we aimed to further assess the consequences upon genetic deletion of AtMICU. Our results confirm that AtMICU safeguards intracellular calcium transport associated with carbohydrate, amino acid, and phytol metabolism modifications. The implications of such alterations are discussed.

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“…Recent reports have successfully measured Mitochondrial Calcium Uniporter-dependent calcium uptake activity either using electrophysiology of reconstituted MCU or through heterologous expression of MCU in yeast [2,3]. Here we present methodological details to assess in situ mitochondrial respiration and calcium uptake in permeabilized pollen tubes [4]. This method could be applied -in principle- to pollen tubes from any plant provided adequate germination and assay buffers are used throughout the process.…”

Section: Methods Detailsmentioning

confidence: 99%

A simple method for mitochondrial respiration and calcium uptake assessment in pollen tubes

et al. 2019

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In situ assessment of mitochondrial calcium transport in tobacco pollen tubes

Cited by 7 publications

References 40 publications

Multi-omics research on common allergens during the ripening of pollen and poplar flocs of Populus deltoides

Multi-omics research on common allergens during the ripening of pollen and poplar flocs of Populus deltoides

An Arabidopsis mutant line lacking the mitochondrial calcium transport regulator MICU shows an altered metabolite profile

A simple method for mitochondrial respiration and calcium uptake assessment in pollen tubes

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