In situ analysis of variations of arsenicals, microbiome and transcriptome profiles along murine intestinal tract

Liu, Xin; Wang, Jiating; Deng, Hongyu; Zhong, Xiaoni; Li, Chengji; Luo, Yu; Chen, Linkang; Zhang, Bin; Wang, Dongbin; Huang, Yongfeng; Zhang, Jingjing; Guo, Lianxian

doi:10.1016/j.jhazmat.2021.127899

Cited by 9 publications

(5 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a limitation of our study is the absence of measurement of SCFA concentrations, and the effects of Cd on SCFAs productions and SCFA-related bacteria in the gut of ruminants need further investigation. Heavy metals can induce intestinal mucosal damage [5,11] and alter the microenvironment for microbes, changing their microbial structures [36]. However, in our study, the morphology of the rumen, jejunum, and colon of cattle did not change after ingestion of Cd-accumulated maize silage.…”

Section: Discussioncontrasting

confidence: 67%

“…Microbial interactions are always present when the intestines are under healthy conditions, and exposure to heavy metals can disturb these interactions [36]. In this study, we found that Cd-accumulated maize silage enhanced bacterial networks in the rumen, jejunum, and colon, suggesting the improvement of microbial correlations, which might be the reason for the increased feed efficiency.…”

Section: Discussionmentioning

confidence: 59%

See 1 more Smart Citation

Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile

Xu,

Yang

et al. 2023

Animals

View full text Add to dashboard Cite

Cadmium (Cd) is an environmental pollutant, widely existing in soil, and can be absorbed and accumulated by plants. Hunan Province exhibits the worst cadmium contamination of farmland in China. Ruminants possess an abundant microbial population in the rumen, which enables them to tolerate various poisonous plants. To investigate whether the rumen microbiota could respond to Cd and mitigate the toxicity of Cd-accumulated maize to ruminants, 6-month-old cattle were fed with 85.82% (fresh basis) normal whole-plant maize silage diet (CON, n = 10) or Cd-accumulated whole-plant maize silage diet (CAM, n = 10) for 107 days. When compared to the CON cattle, CAM cattle showed significantly higher gain-to-feed ratio and an increased total bacterial population in the rumen, but a decreased total bacterial population in the colon. CAM cattle had higher relative abundance of Prevotella and Lachnospiraceae ND3007 group in the rumen, and Lachnospiraceae NK4A136 group and Clostridia vadinBB60 group in the colon. Notably, microbial correlations were enhanced in all segments of CAM cattle, especially Peptostreptococcaceae in the jejunum. Transcriptome analysis revealed down-regulation of several immune-related genes in the rumen of CAM cattle, and differentially expressed genes in the rumen were mostly involved in immune regulation. These findings indicated that feeding Cd-accumulated maize diet with a Cd concentration of 6.74 mg/kg dry matter (DM) could stimulate SCFA-related bacteria in the rumen, induce hormesis to promote weight gain, and improve energy utilization of cattle.

show abstract

Section: Discussioncontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 59%

Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile

Xu,

Yang

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

“…For vertebrates, As3mt is mainly expressed and play roles in the liver, and their gut microbiome can produce As3mt in the intestine − By using food arsenic (predominantly with iAs III ) exposure on mice, we found that along the longitudinal axis of the digestive tract, highly toxic iAs III gradually decreased, while methyl As gradually increased. , Arsenic can also induce the prosperity of As-metabolizing bacteria, such as Seminibacterium and Metallobacterium . , By applying the fecal microbiota transplantation, intestinal microbiome, especially Faecalibacterium, were proved to protect As3mt-KO mice from acute As exposure-induced death . However, the exact role and mechanism of intestinal flora in aiding As biotransformation in vivo remain poorly understood.…”

Section: Introductionmentioning

confidence: 91%

“…27−29 By using food arsenic (predominantly with iAs III ) exposure on mice, we found that along the longitudinal axis of the digestive tract, highly toxic iAs III gradually decreased, while methyl As gradually increased. 30,31 Arsenic can also induce the prosperity of As-metabolizing bacteria, such as Seminibacterium and Metallobacterium. 32,33 By applying the fecal microbiota transplantation, intestinal microbiome, especially Faecalibacterium, were proved to protect As3mt-KO mice from acute As exposure-induced death.…”

Section: Introductionmentioning

confidence: 99%

Effects of Intestinal Microbiota on the Biological Transformation of Arsenic in Zebrafish: Contribution and Mechanism

Zhong,

Zhang,

Huang

et al. 2024

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Both the gut microbiome and their host participate in arsenic (As) biotransformation, while their exact roles and mechanisms in vivo remain unclear and unquantified. In this study, as3mt −/− zebrafish were treated with tetracycline (TET, 100 mg/ L) and arsenite (iAs III ) exposure for 30 days and treated with probiotic Lactobacillus rhamnosus GG (LGG, 1 × 10 8 cfu/g) and iAs III exposure for 15 days, respectively. Structural equation modeling analysis revealed that the contribution rates of the intestinal microbiome to the total arsenic (tAs) and inorganic As (iAs) metabolism approached 44.0 and 18.4%, respectively. Compared with wild-type, in as3mt −/− zebrafish, microbial richness and structure were more significantly correlated with tAs and iAs, and more differential microbes and microbial metabolic pathways significantly correlated with arsenic metabolites (P < 0.05). LGG supplement influenced the microbial communities, significantly up-regulated the expressions of genes related to As biotransformation (gss and gst) in the liver, down-regulated the expressions of oxidative stress genes (sod1, sod2, and cat) in the intestine, and increased arsenobetaine concentration (P < 0.05). Therefore, gut microbiome promotes As transformation and relieves As accumulation, playing more active roles under iAs stress when the host lacks key arsenic detoxification enzymes. LGG can promote As biotransformation and relieve oxidative stress under As exposure.

show abstract

“…On the one hand, human gut bacteria biochemically metabolize arsenic-containing compounds to mitigate the toxicity of arsenic to the host. It was reported that the microbiome protected host from arsenic-induced mortality in mouse models, and microbiome stability [ 30 ] and the presence of specific bacterial such as Faecalibacterium biofidobactrium and Lactobacillus were the main reasons for this protection [ 31 ]. On the other hand, arsenic toxicity can be exacerbated by the gut microbiome.…”

Section: Introductionmentioning

confidence: 99%

Arsenic-Containing Medicine Treatment Disturbed the Human Intestinal Microbial Flora

Chen

Zhao

et al. 2023

Toxics

View full text Add to dashboard Cite

Human intestinal microbiome plays vital role in maintaining intestinal homeostasis and interacting with xenobiotics. Few investigations have been conducted to understand the effect of arsenic-containing medicine exposure on gut microbiome. Most animal experiments are onerous in terms of time and resources and not in line with the international effort to reduce animal experiments. We explored the overall microbial flora by 16S rRNA genes analysis in fecal samples from acute promyelocytic leukemia (APL) patients treated with arsenic trioxide (ATO) plus all-trans retinoic acid (ATRA). Gut microbiomes were found to be overwhelmingly dominated by Firmicutes and Bacteroidetes after taking medicines containing arsenic in APL patients. The fecal microbiota composition of APL patients after treatment showed lower diversity and uniformity shown by the alpha diversity indices of Chao, Shannon, and Simpson. Gut microbiome operational taxonomic unit (OTU) numbers were associated with arsenic in the feces. We evaluated Bifidobacterium adolescentis and Lactobacillus mucosae to be a keystone in APL patients after treatment. Bacteroides at phylum or genus taxonomic levels were consistently affected after treatment. In the most common gut bacteria Bacteroides fragilis, arsenic resistance genes were significantly induced by arsenic exposure in anaerobic pure culture experiments. Without an animal model, without taking arsenicals passively, the results evidence that arsenic exposure by drug treatment is not only associated with alterations in intestinal microbiome development at the abundance and diversity level, but also induced arsenic biotransformation genes (ABGs) at the function levels which may even extend to arsenic-related health outcomes in APL.

show abstract

In situ analysis of variations of arsenicals, microbiome and transcriptome profiles along murine intestinal tract

Cited by 9 publications

References 57 publications

Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile

Assessing the Effects of Dietary Cadmium Exposure on the Gastrointestinal Tract of Beef Cattle via Microbiota and Transcriptome Profile

Effects of Intestinal Microbiota on the Biological Transformation of Arsenic in Zebrafish: Contribution and Mechanism

Arsenic-Containing Medicine Treatment Disturbed the Human Intestinal Microbial Flora

Contact Info

Product

Resources

About