In situ, amplification-free double-stranded mutation detection at 60 copies/ml with thousand-fold wild type in urine

Kirimli, Ceyhun E.; Lin, Selena Y.; Su, Ying‐Hsiu; Shih, Wei‐Heng

doi:10.1016/j.bios.2018.07.062

Cited by 7 publications

(5 citation statements)

References 51 publications

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“…The PMN-PT piezoelectric plate sensor was also able to detect double-stranded HBVDM and K-ras point mutation with a detection efficiency of 70% or better at concentrations as low as 10 −19 m (10 −16 mol m −3 ) against singlestranded mutation detection at the same concentrations. [95] [92] Copyright 2011, Elsevier.…”

Section: Hepatitis B Virus: Group VIImentioning

confidence: 99%

A Review of Piezoelectric and Magnetostrictive Biosensor Materials for Detection of COVID‐19 and Other Viruses

et al. 2020

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fever with or without chills, chest tightness, dry cough, and shortness of breath, while developing patchy to diffuse infiltration of the lungs, as shown radiographically in Figure 1. Identifying and monitoring SARS-CoV-2 infection has become crucially important. A recent projection of the transmission dynamics of SARS-CoV-2 [2] showed that longitudinal serological studies are desperately needed to determine the extent and duration of immunity to SARS-CoV-2. Even in the event of apparent elimination, maintenance of SARS-CoV-2 surveillance is still needed because of a possible resurgence of contagion. In the past, notable viruses have emerged suddenly from obscurity or anonymity, provoking concern from the point of view of immunology regarding their sustained epidemic transmission in naive human populations. More than 70% of these infections have been zoonotic, entering either directly from wild animal reservoirs or indirectly via an intermediate domestic animal host. [3] Ebola virus, avian influenza, human immunodeficiency virus (HIV), and SARS are all examples of zoonoses that have emerged from wild animals, presenting an increasingly serious threat to human health and economies worldwide. Figure 2 shows the trend in the number of people infected with SARS-CoV-2. [4] The spread of the severe acute respiratory syndrome coronavirus has changed the lives of people around the world with a huge impact on economies and societies. The development of wearable sensors that can continuously monitor the environment for viruses may become an important research area. Here, the state of the art of research on biosensor materials for virus detection is reviewed. A general description of the principles for virus detection is included, along with a critique of the experimental work dedicated to various virus sensors, and a summary of their detection limitations. The piezoelectric sensors used for the detection of human papilloma, vaccinia, dengue, Ebola, influenza A, human immunodeficiency, and hepatitis B viruses are examined in the first section; then the second part deals with magnetostrictive sensors for the detection of bacterial spores, proteins, and classical swine fever. In addition, progress related to early detection of COVID-19 (coronavirus disease 2019) is discussed in the final section, where remaining challenges in the field are also identified. It is believed that this review will guide material researchers in their future work of developing smart biosensors, which can further improve detection sensitivity in monitoring currently known and future virus threats.

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Section: Hepatitis B Virus: Group VIImentioning

confidence: 99%

A Review of Piezoelectric and Magnetostrictive Biosensor Materials for Detection of COVID‐19 and Other Viruses

et al. 2020

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“…Moreover, advances in biochips and nanotechnology have led to the development of enzymatic amplification-free protocols for ctDNA 7,19 . Such examples include an electrochemical biochip combined with surface nanostructuring and a clutch/clamp assay 20 ; a plasmonic biosensor employing nanoparticles and a PNA-functionalized gold surface 21 ; and a piezoelectric plate sensor combined with fluorescent reporter microspheres 22 . Overall, the above works present elegant examples of the application of biosensors in PCR-free clinical diagnostics, often achieving impressive sensitivities similar to that of ddPCR 20 .…”

Section: Introductionmentioning

confidence: 99%

Acoustic array biochip combined with allele-specific PCR for multiple cancer mutation analysis in tissue and liquid biopsy

Naoumi

Michaelidou

Papadakis

et al. 2021

Preprint

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Regular screening of cancerous point mutations is of importance to cancer management and treatment selection. Although excellent techniques like next-generation sequencing and droplet digital PCR are available, these are still lacking in speed, simplicity and cost-effectiveness. Here a new approach is presented where allele-specific PCR (AS-PCR) is combined with a novel High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) array biosensor for the amplification and detection, respectively, of cancer point mutations. For the proof-of-concept, the method was applied to the screening of the BRAF V600E and KRAS G12D mutations in spiked-in and clinical samples. Regarding the BRAF target, an analytical sensitivity of 0.01%, i.e., detection of 1 mutant copy of genomic DNA in an excess of 104 wild type molecules, was demonstrated; moreover, quantitative results during KRAS detection were obtained when an optimized assay was employed with a sensitivity of 0.05%. The assays were validated using tissue and plasma samples obtained from melanoma, colorectal and lung cancer patients. Results are in full agreement with Sanger sequencing and droplet digital PCR, demonstrating efficient detection of BRAF and KRAS mutations in samples having an allele frequency below 1%. The high sensitivity and technology-readiness level of the methodology, together with the ability for multiple sample analysis (24 array biochip), cost-effectiveness and compatibility with routine work-flow, hold promise for the implementation of this AS-PCR/acoustic methodology in clinical oncology as a tool for tissue and liquid biopsy.

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Section: Introductionmentioning

confidence: 99%

“…8 Origami DNA is capable of incorporating noble metal nanoparticles, leading to a plasmonic hotspot for fluorescent enhancement. 9 Other amplification-free methods include SERS analysis, 10 piezoelectric plate sensors, 11 and Raman spectroscopy-based methods. 12 Optical and electrochemical detection methods are promising approaches to achieve a sensitive and repeatable assay that is also robust and quick to use.…”

Section: ■ Introductionmentioning

confidence: 99%

Peptide–Fluorophore Hydrogel as a Signal Boosting Approach in Rapid Detection of Cancer DNA

et al. 2019

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Cancer is a major health risk in the modern society that requires rapid, reliable, and inexpensive diagnostics. Because of the low abundance of cancer DNA in biofluids, current detection methods require DNA amplification. The amplification can be challenging; it provides only relative quantification and extends time and cost of an assay. Herein, we report a new oligonucleotide hybridization platform for amplification-free detection of human cancer DNA. Using a large PEG-capture probe allows rapid separation of the bound (mutant) versus unbound (wild type) DNA. Next, a supramolecular hydrogel forming peptide attached to a detection oligonucleotide probe serves as a signal amplification tool. Having screened multiple short peptides and fluorophores, we identified the system P1 + cyanine 3.5 that allows for sensitive quantitative detection of mutation L858R in EGFR oncogene. The peptide–fluorophore-based assay provides absolute target DNA quantification at the detection limit of 20 ng cancer DNA versus >500 ng for Cy3.5-labeled oligonucleotide in only 1 hour.

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In situ, amplification-free double-stranded mutation detection at 60 copies/ml with thousand-fold wild type in urine

Cited by 7 publications

References 51 publications

A Review of Piezoelectric and Magnetostrictive Biosensor Materials for Detection of COVID‐19 and Other Viruses

A Review of Piezoelectric and Magnetostrictive Biosensor Materials for Detection of COVID‐19 and Other Viruses

Acoustic array biochip combined with allele-specific PCR for multiple cancer mutation analysis in tissue and liquid biopsy

Peptide–Fluorophore Hydrogel as a Signal Boosting Approach in Rapid Detection of Cancer DNA

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