In Silico Test and in Vitro Test of Asiatycoside Nanoemulsion Formula as Antibakteri Propionibacterium Acnes

Abstract

Asiaticoside is a triterpenoid glycoside compound obtained from the pegagan plant (Centella asiatica L. Urban). This asiaticoside can be used to heal wounds and reduce reddish acne scars. Asiaticoside has poor absorption and excellent solubility. Nanoemulsion preparations will improve poor absorption of asiaticoside.Nanoemulsion can improve the properties of active compounds with poor absorption with very small particle sizes ranging from 1-100 nm, aiming to increase the permeability of active substances and improve absorption. This research aims to determine the in-silico test of the asiaticoside nanoemulsion formula against androgen receptor protein molecules in the pathogenesis of Propionibacterium acnes (PDB ID: 1VJY) and to determine the in vitro test of the asiaticoside nanoemulsion formula against the Propionibacterium acnes using the disc diffusion method. Based on the results of the in-silico test, it can be predicted that the asiaticoside compound has the potential as an antibacterial with a protein binding mechanism (1VJY), and the asiaticoside nanoemulsion formula has antibacterial activity against Propionibacterium acnes.