In-silico study of SARS-CoV-2 and SARS with special reference to intra-protein interactions, A plausible explanation for stability, divergency and severity of SARS-CoV-2

Abstract

The current nightmare for the whole world is COVID-19. The occurrence of concentrated pneumonia cases in Wuhan city, Hubei province of China was first reported on December 30, 2019. SARS-CoV first discloses in 2002, but not outspread worldwide. After 18 years, in 2020, it reemerges and outspread worldwide as SARS-CoV-2 (COVID 19), as the most treacherous virus creating disease in the world. Is it possible to create a favorable evolution within this (18 years) short time? If possible, then what are those properties or factors that are changed in SARS-CoV-2 to make it undefeated? What are the fundamental differences between SARS-CoV-2 and SARS? This study will find all those queries. Here, we took 4 types of protein sequences from SARS-CoV-2 and SARS are retrieved from the database to check their physicochemical and structural properties. Results showed that charged residues are playing a pivotal role in SARS-CoV-2 evolution. Those charged residues also contribute to helix stabilization of SARS-CoV-2. Formation of cyclic salt bridge and other intra-protein interactions also play crucial role in SAS-CoV-2. This comparative study will help to understand the evolution from SARS to SARS-CoV-2 and also helps in protein engineering.

Preliminary identification of potential phytochemical components as antiviral agents against Dengue Virus 2: An <i>in silico</i> study

“…The aliphatic index was 78.72, and the grand average of hydropathicity (GRAVY) was -0.392. The aliphatic index value indicates the stability of the protein even in high temperatures (Mitra et al, 2020). This data suggested that the envelope protein is very stable in body temperature.…”

Cold adaptation strategy of psychrophilic bacteria: an in-silico analysis of isocitrate dehydrogenase

“…It is reported that two residues are involved in the formation of aromatic-aromatic [52][53][54]. But here, we have divided this aromatic-aromatic interactions into two divisions; isolated aromatic-aromatic interactions and network aromatic-aromatic interactions.…”

Indications and Recommendations for Tracheostomy in Trauma Population With COVID-19 Infection: A Review of the Literature

“…20 Additional insights for dealing with COVID-19 have been drawn from the 2002 SARS-CoV-1 epidemic. [20][21][22] Given the nature of this novel coronavirus, which is spread by respiratory droplet, and the aerosolgenerating nature of tracheostomy placement, especially strict precautions are recommended for COVID-19 patients requiring ventilation. 23,24 Both the American Association for the Surgery of Trauma (AAST) and the Eastern Association for the Surgery of Trauma (EAST) have posted or endorsed guidelines specific to tracheostomy in the setting of COVID-19 on their respective webpages.…”

“…Ideally, placement of a cuffed, non-fenestrated tracheostomy tube should be performed in a negative pressure airborne infection isolation room (AIIR); otherwise, the procedure should be done in a setting isolated for at least 3 hours to minimize transmission of aerosols. 21 Non-essential personnel and additional equipment should be accessible but not in the room where the procedure is being performed.…”