In Silico Study of Anticancer Activity of Red Betel Leaves Bioactive Compounds against Colon Cancer Marker Proteins

Abstract

Colon cancer or colorectal cancer is one of the major health problems in the world. Previous research has proven that red betel leaves extract has anticancer activity against breast cancer cells. The study aimed to look at the potential of compounds contained in red betel ethyl acetate fraction as anticancer substances for colon cancer by conducting in silico tests through the docking of three colon cancer biomarker receptors against eight red betel leaves compounds. The parameters of binding affinity energy and inhibition constant used in the molecular docking analysis showed that there are several compounds that have the potential as inhibitors against colon cancer marker proteins to inhibit the development of colorectal cancer and have high bioavalibility as oral drugs. Based on in silico test it is known that the compound N-1,N-9-Bis [E-(2-nitophenyl) methylene] non anedihydrazide has the highest inhibition of human leukotriene A4 hydrolase receptor with binding affinity energy of 11.3160 Kcal/mol. The next compound is 4-({4,6-Bis[3R,5S)-3,5-diamino-1-piperydinyl]-1,3,5-traizin-2-yl}amino) benzenosulfon amide which has the highest inhibition in Chek1 with binding affinity energy of 9.7110 Kcal/mol, and the compound 4-(4-methoxy-phenylamino)-2,3-dihydro-1H-4a, 9-diaza-cyclopenta (b) fluorine-10-carbonitrile has the highest inhibition in the Bcl 2 navitoclax analog receptor with binding affinity energy of 8.4470 Kcal/mol.

Total Phenolic Content and Proliferation Activity of Spirulina Extract in Lymphocyte Cell

“…The OD value is directly proportional to the viability value, the higher the OD value the higher the viability value. Viability is the percentage value of cell survival obtained from the OD value of the sample absorbance reading [16].…”

Clusterization of Red Betel Leaves (Piper Crocatum) from Various Regions in Indonesia Based on Secondary Metabolite Fingerprint Analysis and Cytotoxicity Values

“…The active compounds contained in the red betel plant. This plant to have much potential to treat various diseases, including its potential as an antioxidant (Safithri et al, 2022), anti-inflammatory (Gong et al, 2021), antihyperglycemic (Hasibuan et al, 2016), anticancer by inhibiting the proliferation of cancer cells (Zulharini et al, 2018), anticancer colon in silico studies (Umar et al, 2023) antiviral (Diniatik, 2011), antiobesity (Apritya et al, 2020), antibacterial (Mangesa & Irsan, 2020) and also as an antidiabetic (Maha et al, 2020). Saraswati and Palupi (2019) show that red betel leaves collected from Kupang, East Nusa Tenggara contain 35 compounds with % quality > 90%, and are dominated by 5 compound components with the largest % area, namely β-Mirsen (13.80%), Linalool (3.29%), α-Thujen (1.52%), γ-Terpinen (1.36%), cis-β-Terpineol (1.15%).…”