In silico prediction of human genes as potential targets for rice miRNAs

Identification of potential target genes in Homo sapiens, by miRNA of Triticum aestivum: A cross kingdom computational approach

“…Identifying mammalian gene targets in a timely manner by plant miRNA offers a reliable, effective and economical way to determine the possible effects of interactions given by exogenous molecules, regardless of their origin, on human mRNA [ [ [9] , [10] , [11] , [12] , [13] , [14] ]].…”

The Challenges and Opportunities in the Development of MicroRNA Therapeutics: A Multidisciplinary Viewpoint

“…In another study, the miR containing microvesicles isolated from Moringa oleifera (Drumstick) were bioinformatically predicted to target apoptosis-related human genes, and its exposure decreased the cancer cell viability and increased apoptosis [172]. From rice, multiple miRs (e.g., osa-miR156-5p and osa-miR164-5p) are predicted to target human genes [173]. Chin et al reported the presence of plant miR159 in human serum and showed that its levels inversely correlated with breast cancer incidence and progression [165].…”

Identification of Bovine miRNAs with the Potential to Affect Human Gene Expression

“…In recent years, numerous studies have shown that the milk of humans and cows is enriched with miRNAs (Chen et al, 2010;Weber et al, 2010), most of which are packed in extracellular vesicles that are 30-120 nm in diameter, namely exosomes, which are derived from all types of cells and released into all biological fluids, such as blood plasma, serum, urine, breast milk, colostrum, and more (Kosaka et al, 2010;Gu et al, 2012;Xiao et al, 2018;Yun et al, 2021;Zeng et al, 2020;Zeng et al,2021). Zhang et al suggested that exogenous miRNAs (ex-miRs), specifically from plants, can withstand the digestion process, enter the animal's bloodstream through the gastrointestinal tract, and regulate gene expression (Zhang L. et al, 2012, Zhang et al, 2012Dickinson et al, 2013;Laubier et al, 2015;Title et al, 2015;Auerbach et al, 2016;Rakhmetullina et al, 2020). It was shown that when piglets were fed pig or cow's milk, miRNAs could be absorbed both in vivo and in vitro, which creates the basis for understanding the participation of miRNAs in physiological functions (Lin et al, 2020).…”

“…Zhang et al suggested that exogenous miRNAs (ex-miRs), specifically from plants, can withstand the digestion process, enter the animal’s bloodstream through the gastrointestinal tract, and regulate gene expression ( Zhang L. et al, 2012 , Zhang et al, 2012 Y. ; Dickinson et al, 2013 ; Laubier et al., 2015 ; Title et al, 2015 ; Auerbach et al, 2016 ; Rakhmetullina et al, 2020 ). It was shown that when piglets were fed pig or cow’s milk, miRNAs could be absorbed both in vivo and in vitro , which creates the basis for understanding the participation of miRNAs in physiological functions ( Lin et al, 2020 ).…”

“…Research in the field of dietary miRNAs has shifted away from miRNAs in plant-borne foods ( Rakhmetullina et al, 2020 ) to miRNAs in foods of animal origin, particularly cow’s milk, after observations that a large proportion of milk miRNAs is encapsulated in extracellular vesicles such as exosomes. The miRNAs encapsulated in milk exosomes are stable under harsh conditions, such as low pH and exposure to ribonucleases ( Kosaka et al, 2010 ; Izumi et al, 2012 ).…”