In silico Neuropeptidome of Female Macrobrachium rosenbergii Based on Transcriptome and Peptide Mining of Eyestalk, Central Nervous System and Ovary

Abstract

Macrobrachium rosenbergii is the most economically important of the cultured freshwater crustacean species, yet there is currently a deficiency in genomic and transcriptomic information for research requirements. In this study, we present an in silico analysis of neuropeptide genes within the female M. rosenbergii eyestalk, central nervous system, and ovary. We could confidently predict 37 preproneuropeptide transcripts, including those that encode bursicons, crustacean cardioactive peptide, crustacean hyperglycemic hormones, eclosion hormone, pigment-dispersing hormones, diuretic hormones, neuropeptide F, neuroparsins, SIFamide, and sulfakinin. These transcripts are most prominent within the eyestalk and central nervous system. Transcript tissue distribution as determined by reverse transcription-polymerase chain reaction revealed the presence of selected neuropeptide genes of interest mainly in the nervous tissues while others were additionally present in the non-nervous tissues. Liquid chromatography-mass spectrometry analysis of eyestalk peptides confirmed the presence of the crustacean hyperglycemic hormone precursor. This data set provides a strong foundation for further studies into the functional roles of neuropeptides in M. rosenbergii, and will be especially helpful for developing methods to improve crustacean aquaculture.

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

“…Filtering was conducted by removing transcripts of (a) RPKM <1 in all tissues, suggesting them to be either poorly assembled and therefore showing consistent low coverage or evidence of broad basal expression and (b) transcripts of length <500 nucleotides (nt) which probably account for partial sequences, guided by the fact that even the shortest transcripts which encode neuropeptides exceed this length [Ventura et al, 2014b;Manfrin et al, 2015;Suwansa-Ard et al, 2015]. All subsequent DEA were conducted using this filtered library.…”

“…These neuropeptides include the putative regualtors gonad inhibiting hormone (GIH) aka VIH (vitellogeneis inhibiting hormone in females), molt-inhibiting hormone (MIH), and crustacean hyperglycemic hormone (CHH) [Rodríguez et al, 2007;Nagaraju, 2011;Webster et al, 2012;Ventura et al, 2014b;Manfrin et al, 2015;Suwansa-Ard et al, 2015]. Recently, the influence of these neurohormones has been explicitly demonstrated.…”

Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

“…By binding to receptors in target tissues, neuropeptides elicit a number of downstream cascades including changes in secondary messengers, phosphorylation and eventually altered transcription activity which leads to a biological response4. In silico neuropeptidome research aimed at understanding different physiological aspects of crustacean biology has been extensively studied in several crustacean species, including Scylla paramamosain 5, Macrobrachium rosenbergii 6, Procambarus clarkii 7, Homarus americanus and Sagmariasus verreauxi 89.…”

Neuropeptide modulation of pattern-generating systems in crustaceans: comparative studies and approaches

“…Moreover, errors in sequences can be artifacts of the transcriptome assembly process, and post-translational modifications are merely predictions. Some studies counter this dilemma by combining transcriptomics and mass spectrometry approaches to confirm some of the mined sequences [19,20*,21]. Studies such as these, although interesting in their own right, are largely lacking significant physiological relevance.…”