In silico molecular docking, molecular dynamics, ADMET analysis of fucoidan against receptor frizzled-8 and coreceptor LRP6 in Wnt/β-Catenin pathway and in vitro analysis of fucoidan extract from
            <i>Sargassum echinocarpum</i>
            as β-catenin inhibitor in breast cancer cell line (MCF-7)

Laeliocattleya, Rosalina Ariesta; Yunianta, Yunianta; Risjani, Yenny; Wulan, Siti Narsito

doi:10.1080/07391102.2023.2265488

Cited by 2 publications

(1 citation statement)

References 48 publications

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“…This remains to be investigated in the future. Such analyses require various techniques to analyze RS and its effects of the body, for example, molecular docking prediction, analysis using RS-specific antibodies or radioisotope-labeled RS, and experiments, such as pull-down assays and affinity chromatography, should be conducted, as performed by Laeliocattleya et al [50] in their research on fucoidan.…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

Terasawa,

Zang,

Hiramoto

et al. 2023

Cells

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Oral administration of rhamnan sulfate (RS), derived from the seaweed Monostroma nitidum, markedly suppresses inflammatory damage in the vascular endothelium and organs of lipopolysaccharide-treated mice. This study aimed to analyze whether orally administered RS inhibits the development of atherosclerosis, a chronic inflammation of the arteries. ApoE-deficient female mice were fed a normal or high-fat diet (HFD) with or without RS for 12 weeks. Immunohistochemical and mRNA analyses of atherosclerosis-related genes were performed. The effect of RS on the migration of RAW264.7 cells was also examined in vitro. RS administration suppressed the increase in blood total cholesterol and triglyceride levels. In the aorta of HFD-fed mice, RS reduced vascular smooth muscle cell proliferation, macrophage accumulation, and elevation of VCAM-1 and inhibited the reduction of Robo4. Increased mRNA levels of Vcam1, Mmp9, and Srebp1 in atherosclerotic areas of HFD-fed mice were also suppressed with RS. Moreover, RS directly inhibited the migration of RAW264.7 cells in vitro. Thus, in HFD-fed ApoE-deficient mice, oral administration of RS ameliorated abnormal lipid metabolism and reduced vascular endothelial inflammation and hyperpermeability, macrophage infiltration and accumulation, and smooth muscle cell proliferation in the arteries leading to atherosclerosis. These results suggest that RS is an effective functional food for the prevention of atherosclerosis.

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Section: Discussionmentioning

confidence: 99%

Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

Terasawa,

Zang,

Hiramoto

et al. 2023

Cells

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Fucoidan extract from brown seaweed ( Sargassum echinocarpum ): molecular weight, elemental composition, selectivity and anticancer activity against breast cancer cells

Yunianta,

Laeliocattleya,

Wulan

et al. 2024

Natural Product Research

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Molecular weight and elemental composition contribute to fucoidan bioactivity. Fucoidan extract from S. echinocarpum had three fractions varying in molecular weights (Mw), whereas crude fucoidan extract had a moderate Mw of 2,034.31 kDa. The fucoidan extract contained several elements, including C (38.19%), O (44.46%), and S (2.61%). Moreover, the fucoidan extract exhibited toxicity to breast cancer cells (MCF-7) at 297.58±2.40 ppm. The extract induced apoptosis by 49.78%, 72.05%, and 89.35% after incubation for 24, 48, and 72 hours, respectively. Fucoidan increased MDA levels in MCF-7 cells, indicating its anticancer properties through the induction of apoptosis-induced lipid peroxidation. Furthermore, the anticancer activity of fucoidan extract was not significantly different from the standard fucoidan (F. vesiculosus). In addition, the extract was selective and non-toxic to human normal cells (TIG 1-20), indicating its safety for consumption and potential as an anticancer agent derived from marine algae.

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In silico molecular docking, molecular dynamics, ADMET analysis of fucoidan against receptor frizzled-8 and coreceptor LRP6 in Wnt/β-Catenin pathway and in vitro analysis of fucoidan extract from Sargassum echinocarpum as β-catenin inhibitor in breast cancer cell line (MCF-7)

Cited by 2 publications

References 48 publications

Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

Fucoidan extract from brown seaweed ( Sargassum echinocarpum ): molecular weight, elemental composition, selectivity and anticancer activity against breast cancer cells

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