In-silico functional analysis of hypothetical proteins from Lactiplantibacillus plantarum plasmids reveals enrichment of cell envelope proteins

Davray, Dimple; Kulkarni, Ram

doi:10.1016/j.plasmid.2023.102693

Cited by 1 publication

(1 citation statement)

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“…Successful translation of these products depends on robust yields of secreted proteins which mainly relies on the choice of signal peptides coupled to the protein. A study by and colleagues involving functional analysis of 647 hypothetical proteins of Lactiplantibacillus plantarum predicted by bioinformatic tools, revealed 112 transporter protein families and 40 protein families comprised of homologous signal peptides (Davray & Kulkarni, 2023 ). Mathiesen and colleagues also tested the secretion capability of 76 native signal peptides in L. plantarum WCFS1 using staphylococcal nuclease (nucA) as the reporter protein (Mathiesen et al, 2009 ).…”

Section: Inducible Gene Expression Systemsmentioning

confidence: 99%

Expanding the genetic programmability of Lactiplantibacillus plantarum

Blanch‐Asensio,

Dey,

Tadimarri

et al. 2023

Microbial Biotechnology

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Lactobacilli are ubiquitous in nature and symbiotically provide health benefits for countless organisms including humans, animals and plants. They are vital for the fermented food industry and are being extensively explored for healthcare applications. For all these reasons, there is considerable interest in enhancing and controlling their capabilities through the engineering of genetic modules and circuits. One of the most robust and reliable microbial chassis for these synthetic biology applications is the widely used Lactiplantibacillus plantarum species. However, the genetic toolkit needed to advance its applicability remains poorly equipped. This mini‐review highlights the genetic parts that have been discovered to achieve food‐grade recombinant protein production and speculates on lessons learned from these studies for L. plantarum engineering. Furthermore, strategies to identify, create and optimize genetic parts for real‐time regulation of gene expression and enhancement of biosafety are also suggested.

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Section: Inducible Gene Expression Systemsmentioning

confidence: 99%