In silico epitope prediction and immunogenic analysis for penton base epitope-focused vaccine against hydropericardium syndrome in chicken

Aziz, Faiza; Tufail, Soban; Shah, Majid Ali; Shah, Muhammad Arif; Habib, Md. Ahasan; Mirza, Osman; Iqbal, Mazhar; Rahman, Mahmuder

doi:10.1016/j.virusres.2019.197750

Cited by 17 publications

(22 citation statements)

References 61 publications

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“…Due to the lack of chicken MHC alleles, human MHC alleles were used to predict the epitopes. Earlier studies used human alleles because of the lack of chicken alleles in immunoinformatics online tools. ,, Studies have shown that chicken B–F alleles can stimulate an immune response similar to human class I homologous alleles, particularly in antigen presentation. , Due to the lack of chicken MHC-I and MHC-II alleles in the T-cell epitope prediction database, the closest similar human alleles (HLA*B 40:06, HLA*B 41:04, and HLA*B 41:03 for MHC I and DRB1:1482, DRB1:1366, DRB1:1310, and DRB1:1445 for MHC II) were used. The NetMHCcons 1.1 server predicted MHC-I binding epitopes.…”

Section: Resultsmentioning

confidence: 99%

Multi-epitope-Based Vaccine Designed by Targeting Cytoadherence Proteins of Mycoplasma gallisepticum

Mugunthan

Harish

2021

ACS Omega

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Mycoplasma gallisepticum causes chronic respiratory disease in chickens leading to large economic losses in the poultry industry, and the impacts remain to be a great challenge for a longer period. Among the other approaches, a vaccine targeting the adhesion proteins of M. gallisepticum would be a promising candidate in controlling the infection. Thus, the present study is aimed to design a multi-epitope vaccine candidate using cytoadhesion proteins of M. gallisepticum through an advanced immunoinformatics approach. As a result, the multi-epitope vaccine was constructed, which comprised potential T-cell and B-cell binding epitopes with appropriate adjuvants. The designed multi-epitope vaccine represented high antigenicity with viable physiochemical properties. The prospective three-dimensional structure of the epitope was predicted, refined, and validated. The molecular docking analysis of multi-epitope vaccine candidates with the chicken Toll-like receptor-5 predicted effective binding. Furthermore, codon optimization and in silico cloning ensured high expression. Thus, the present finding indicates that the engineered multi-epitope vaccine is structurally stable and can induce a strong immune response. Furthermore, the multi-epitope vaccine is suggested to be a suitable vaccine candidate for the M. gallisepticum infection due to its effective binding capacity and precise specificity.

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Section: Resultsmentioning

confidence: 99%

Multi-epitope-Based Vaccine Designed by Targeting Cytoadherence Proteins of Mycoplasma gallisepticum

Mugunthan

Harish

2021

ACS Omega

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“…The lack of chicken MHC alleles in the immunoinformatics database was a major drawback; thus, human MHC alleles were substituted for chicken alleles to predict the epitopes. Previous studies used human alleles to predict epitopes against poultry pathogens ( 27 , 28 ). Reports have revealed that chicken B–F alleles have the capacity to induce immune response comparable with human class I alleles predominantly during antigen presentation ( 29 , 30 ).…”

Section: Methodsmentioning

confidence: 99%

A Computational Reverse Vaccinology Approach for the Design and Development of Multi-Epitopic Vaccine Against Avian Pathogen Mycoplasma gallisepticum

Mugunthan

Chandra

2021

Front. Vet. Sci.

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Avian mycoplasma is a bacterial disease causing chronic respiratory disease (CRD) in poultry industries with high economic losses. The eradication of this disease still remains as a challenge. A multi-epitope prophylactic vaccine aiming the antigenic proteins of Mycoplasma gallisepticum can be a capable candidate to eradicate this infection. The present study is focused to design a multi-epitope vaccine candidate consisting of cytotoxic T-cell (CTL), helper T-cell (HTL), and B-cell epitopes of antigenic proteins, using immunoinformatics strategies. The multi-epitopic vaccine was designed, and its tertiary model was predcited, which was further refined and validated by computational tools. After initial validation, molecular docking was performed between multi-epitope vaccine construct and chicken TLR-2 and 5 receptors, which predicted effective binding. The in silico results specify the structural stability, precise specificity, and immunogenic response of the designed multi-epitope vaccine, and it could be an appropriate vaccine candidate for the M. gallisepticum infection.

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“…The nonamers were analysed using the Stabilized Matrix Base Method (SMM). The parameters for identifying MHC-I binding alleles selected included the amino acid length of peptide set to 9.0, the IC 50 value < 250, and the human as MHC source species 21 , 34 . The generated epitopes were examined for antigenicity, with a threshold set to 0.5 as the main parameter.…”

Section: Methodsmentioning

confidence: 99%

Designing multiepitope-based vaccine against Eimeria from immune mapped protein 1 (IMP-1) antigen using immunoinformatic approach

Madlala

Adeleke

Fatoba

et al. 2021

Sci Rep

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Drug resistance against coccidiosis has posed a significant threat to chicken welfare and productivity worldwide, putting daunting pressure on the poultry industry to reduce the use of chemoprophylactic drugs and live vaccines in poultry to treat intestinal diseases. Chicken coccidiosis, caused by an apicomplexan parasite of Eimeria spp., is a significant challenge worldwide. Due to the experience of economic loss in production and prevention of the disease, development of cost-effective vaccines or drugs that can stimulate defence against multiple Eimeria species is imperative to control coccidiosis. This study explored Eimeria immune mapped protein-1 (IMP-1) to develop a multiepitope-based vaccine against coccidiosis by identifying antigenic T-cell and B-cell epitope candidates through immunoinformatic techniques. This resulted in the design of 7 CD8+, 21 CD4+ T-cell epitopes and 6 B-cell epitopes, connected using AAY, GPGPG and KK linkers to form a vaccine construct. A Cholera Toxin B (CTB) adjuvant was attached to the N-terminal of the multiepitope construct to improve the immunogenicity of the vaccine. The designed vaccine was assessed for immunogenicity (8.59968), allergenicity and physiochemical parameters, which revealed the construct molecular weight of 73.25 kDa, theoretical pI of 8.23 and instability index of 33.40. Molecular docking simulation of vaccine with TLR-5 with binding affinity of − 151.893 kcal/mol revealed good structural interaction and stability of protein structure of vaccine construct. The designed vaccine predicts the induction of immunity and boosted host's immune system through production of antibodies and cytokines, vital in hindering surface entry of parasites into host. This is a very important step in vaccine development though further experimental study is still required to validate these results.

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In silico epitope prediction and immunogenic analysis for penton base epitope-focused vaccine against hydropericardium syndrome in chicken

Cited by 17 publications

References 61 publications

Multi-epitope-Based Vaccine Designed by Targeting Cytoadherence Proteins of Mycoplasma gallisepticum

Multi-epitope-Based Vaccine Designed by Targeting Cytoadherence Proteins of Mycoplasma gallisepticum

A Computational Reverse Vaccinology Approach for the Design and Development of Multi-Epitopic Vaccine Against Avian Pathogen Mycoplasma gallisepticum

Designing multiepitope-based vaccine against Eimeria from immune mapped protein 1 (IMP-1) antigen using immunoinformatic approach

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